A5079515428- Cardiac Fibrosis and Remodeling
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Glioma Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Ovarian cancer diagnosis and treatment
- Service-Oriented Architecture and Web Services
- Immune cells in cancer
- CAR-T cell therapy research
- Brain Metastases and Treatment
- Cancer-related Molecular Pathways
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Generative Adversarial Networks and Image Synthesis
- Trauma and Emergency Care Studies
- Lymphatic System and Diseases
- Smart Agriculture and AI
- Gene Regulatory Network Analysis
- 3D Shape Modeling and Analysis
- Bacillus and Francisella bacterial research
- Microtubule and mitosis dynamics
University of Virginia Health System
2022-2025
University of Pennsylvania
2025
Tianjin University
2024
Southwest University
2024
University of Virginia
2003-2023
City College of Dongguan University of Technology
2019
Abstract Cancer cell dissemination to sentinel lymph nodes is associated with poor patient outcomes, particularly in breast cancer. The process by which cancer cells egress from the primary tumor upon interfacing lymphatic vasculature complex and driven dynamic interactions between stromal cells, including cancer-associated fibroblasts (CAF). matricellular protein periostin can distinguish CAF subtypes increased desmoplasia disease recurrence patients. However, as secreted,...
Abstract Although CD8+ T cells are critical for controlling tumors, how they recruited and home to primary metastatic lesions is incompletely understood. We characterized the homing receptor (HR) ligands on tumor vasculature determine what drives their expression role in T-cell entry. The anatomic location of B16-OVA tumors affected E-selectin, MadCAM-1, VCAM-1, whereas HR CXCL9 ICAM-1 were expressed regardless location. VCAM-1 was induced by IFNγ-secreting adaptive immune cells. CXCL9/10...
Brain metastasis is thought to be related the high mortality and poor prognosis of lung cancer. Despite significant advances in treatment primary cancer, unique microenvironment brain renders current therapeutic strategies largely ineffective against metastasis. The lack effective drugs for primarily due incomplete understanding mechanisms underlying its initiation progression. Currently, our remains limited, absence appropriate models that can realistically simulate entire process tumor...
Brain metastases are a leading cause of cancer-related mortality. However, progress in their treatment has been limited over the past decade, due to an incomplete understanding underlying biological mechanisms. Employing accurate
Basal-like breast cancer (BLBC) is highly aggressive, and often characterized by BRCA1 p53 deficiency. Although conventional mouse models enabled the investigation of BLBC at malignant stages, its initiation pre-malignant progression remain understudied. Here, we leveraged a genetic system known as mosaic analysis with double markers (MADM) to study generating rare GFP+Brca1, p53-deficient mammary cells alongside RFP+ wild-type sibling cells. After confirming close resemblance tumors arising...
The catastrophes of September 11, 2001 put Arlington County's emergency response system to the test. They revealed capabilities and limitations otherwise overlooked during previous standard assessments. There were three key issues in 9/11 that are recurring jurisdictions across nation: 1) reliance on voice-oriented communications; 2) limited situational awareness; 3) lack interoperability. A concept operations integrating current commercially available technology a designed for coordinator...
Abstract Surgical resection of glioblastoma multiforme (GBM) often results in tumor recurrence and mild neurologic deficits. Here, a 3D asynchronous drug delivery system is innovatively developed to address the dual challenges GBM postoperative neurological deficit. Based on transcriptome analysis cells microenvironment (TME) between primary recurrent mouse tissues, novel dual‐targeting approach combine mTOR pathway inhibition with microglia/macrophage repolarization. Then, situ injectable...
Different cellular compartments within a tissue present distinct cancer-initiating capacities. Current approaches to dissect such heterogeneity require cell-type-specific genetic tools based on well-understood lineage hierarchy, which are lacking for many tissues. Here, we circumvented this hurdle and revealed the dichotomous capacity of fallopian tube Pax8+ cells in initiating ovarian cancer, utilizing mouse system that stochastically generates rare GFP-labeled mutant cells. Through clonal...
We present a study of the technical feasibility applying peer-to-peer (P2P) technology to domain emergency response. The conceptual such an endeavor is described in "Integrated Peer-to-Peer Applications for Advanced Emergency Response Systems Part I: Concept Operations" (Bahora, et al.), which argues that current response technologies do not adequately support dynamic nature To provide support, networks have been identified as integrating architecture. explore integration architecture,...
In this paper, we present direct numerical simulation (DNS) of the transient dynamics single-molecule DNA by modeling it as a highly flexible cable. Fully nonlinear dynamic equations are derived from principles conservation momentum and angular momentum, solved via means. Compared to previous models, cable-dynamics model enables physical an individual biopolymer string rather than random-walk style statistical modeling. To validate DNS approach, canonical stretching problem is simulated. The...
Abstract Basal-like breast cancer is an aggressive subtype, often characterized by a deficiency in BRCA1 function and concomitant loss of p53 . While conventional mouse models enable the investigation its malignant stages, one that reveals initiation pre-malignant progression lacking. Here, we leveraged genetic system known as M osaic A nalysis with D ouble arkers (MADM) to generate rare GFP-labeled Brca1 , -deficient cells alongside RFP+ wildtype sibling mammary gland. The mosaicism...
Mosaic analysis with double markers (MADM) mouse models closely mimic the clonal origin of human cancers by generating sporadic, GFP-labeled cancer-initiating cells. Traditional pipelines are labor intensive, hindering throughput and disrupting 3D architecture. Here, we present a protocol that integrates tissue clearing light-sheet imaging to analyze pre-malignant clones in whole-mount MADM-labeled tissues. We describe steps for mosaic-labeled cancer models, harvesting, fixation, clearing....
<div>Abstract<p>Cancer cell dissemination to sentinel lymph nodes associates with poor patient outcomes, particularly in breast cancer. The process by which cancer cells egress from the primary tumor upon interfacing lymphatic vasculature is complex and driven dynamic interactions between stromal cells, including associated fibroblasts (CAFs). matricellular protein periostin can distinguish CAF subtypes increased desmoplasia disease recurrence patients. However, since secreted,...
<p>Data supplement</p>
<p>Data supplement</p>
With strong expressive capabilities in Large Language Models(LLMs), generative models effectively capture sentiment structures and deep semantics, however, challenges remain fine-grained classification across multi-lingual complex contexts. To address this, we propose the Sentiment Cross-Lingual Recognition Logic Framework (SentiXRL), which incorporates two modules,an emotion retrieval enhancement module to improve accuracy contexts through historical dialogue logical reasoning,and a...
We develop a methodology for analyzing risk to the meat supply, with specific focus on slaughterhouses. The safety of nation's supply from terrorist attack has become major concern USDA after September 11/sup th/ attacks Pentagon and World Trade Center. performs assessment through hierarchical holographic modelling (HHM). goal HHM is capture all observables as well identify vulnerabilities target. A 2-player game using blue team red aided in completing more thorough diagram. This process...
<p>S1: LLC-OVA or MC-38-OVA tumors were harvested on day 14 (SC) 11 (IP). flash frozen and stained for MadCAM-1 expression. Representative summary data interrogating 8-10 random fields from single sections of 3 each the percent CD31+ pixels that MadCAM-1+. Percentages calculated using ImageJ to define quantitating those positive MadCAM-1. digested flow cytometry. Summary (n=2 per group) CD31+CD45+ cells expressed E-Selectin. Statistical analysis performed unpaired student's T-test. S2:...
<div>Abstract<p>Although CD8<sup>+</sup> T cells are critical for controlling tumors, how they recruited and home to primary metastatic lesions is incompletely understood. We characterized the homing receptor (HR) ligands on tumor vasculature determine what drives their expression role in T-cell entry. The anatomic location of B16-OVA tumors affected E-selectin, MadCAM-1, VCAM-1, whereas HR CXCL9 ICAM-1 were expressed regardless location. VCAM-1 was induced by...