A5102940619- Synthesis and Catalytic Reactions
- Fish Ecology and Management Studies
- Catalytic C–H Functionalization Methods
- Genetic and phenotypic traits in livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Organic Chemistry Cycloaddition Reactions
- Oxidative Organic Chemistry Reactions
- Asymmetric Hydrogenation and Catalysis
- Genetic diversity and population structure
- Monoclonal and Polyclonal Antibodies Research
- Environmental DNA in Biodiversity Studies
- Immunotherapy and Immune Responses
- Cancer Treatment and Pharmacology
- Animal Genetics and Reproduction
- Phytochemistry and Bioactivity Studies
- Plant-based Medicinal Research
- T-cell and B-cell Immunology
- Wildlife Ecology and Conservation
- Polyoxometalates: Synthesis and Applications
- Chemical Synthesis and Analysis
- Microbial infections and disease research
- Radioactive element chemistry and processing
- Influenza Virus Research Studies
- Asymmetric Synthesis and Catalysis
Itasca Consultants (United States)
2017-2021
Columbia University
2017-2018
University of Wisconsin–Madison
2009-2014
Division of Chemistry
2010
NOAA National Marine Fisheries Service Northwest Fisheries Science Center
2005-2010
Washington Department of Fish and Wildlife
2010
NOAA National Marine Fisheries Service
2005-2009
University of California, Davis
2002-2008
Washington State University Vancouver
2008
National Oceanic and Atmospheric Administration
2005
The regioselective and enantioselective oxyamination of alkenes with N-sulfonyl oxaziridines is catalyzed by a novel iron(II) bis(oxazoline) complex. This process affords oxazolidine products that can be easily manipulated to yield highly enantioenriched free amino alcohols. regioselectivity this complementary obtained from the analogous copper(II)-catalyzed reaction. Thus, both regioisomers 1,2-aminoalcohols using oxaziridine-mediated reactions, overall sense regiochemistry controlled...
We have discovered that N-sulfonyl oxaziridines react with a broad range of olefins in the presence iron salts to afford 1,3-oxazolidines. This process provides access 1,2-aminoalcohols opposite sense regioselectivity produced from copper-catalyzed oxyamination previously reported by our laboratories. Thus, either regioisomeric form can easily be obtained reaction olefins, and controlled appropriate choice inexpensive, nontoxic, first-row transition-metal catalyst.
There is significant debate regarding whether B cells and their antibodies contribute to effective anti-cancer immune responses. Here we show that patients with metastatic but non-progressing melanoma, lung adenocarcinoma, or renal cell carcinoma exhibited increased levels of blood plasmablasts. We used a cell-barcoding technology sequence plasmablast antibody repertoires, revealing clonal families affinity matured exhibit progressive class switching persistence over time. Anti-CTLA4 other...
Abstract Previous studies of population genetic structure fall‐run chinook salmon ( Oncorhynchus tshawytscha ) in California’s Central Valley have either not focused on or been unable to resolve intertributary differences within the San Joaquin River basin. The authors describe isolation, polymerase chain reaction conditions, and characterize cross‐species amplification 17 microsatellite loci six species salmonids. Fourteen these are polymorphic from drainage. These results indicate...
Understanding the relative fitness of naturally spawning hatchery fish compared with wild has become an important issue in management and conservation salmonids. We used a DNA-based parentage analysis to measure reproductive success hatchery- natural-origin spring Chinook salmon ( Oncorhynchus tshawytscha ) natural environment. Size age had large influence on male fitness, larger older males producing more offspring than smaller or younger individuals. significant effect female but was...
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with split-inactivated influenza virus vaccine during 2016–2017 season. A combination Immune Repertoire Capture (IRC TM ) technology and IgG was performed on ~7,800 plasmablast (PB) cells preferential heavy-light chain pairings investigated. some participants, a expanded clonotype...
Abstract We assessed the population genetic structure and temporal stability of diversity from 1999 to 2001 in collections fall‐run Chinook salmon Oncorhynchus tshawytscha California's Central Valley. Tests for genotypic differentiation at seven microsatellite loci revealed few significant pairwise comparisons between samples five hatchery populations eight naturally spawning throughout Valley that were separated by 50–350 km. All genetically homogeneous failed cluster with their nearest...
We have developed a highly selective kinetic resolution of N-sulfonyl oxaziridines. This reaction utilizes an inexpensive and easily synthesized iron bis(oxazoline) catalyst to promote the efficient rearrangement oxaziridines corresponding imides; no sacrificial reagents are required effect this resolution. process is readily translated gram scale, which provides practical method for preparation structurally diverse, enantiopure use as in organic synthesis.
We investigated differences in the statistical power to assign parentage between an artificially propagated and wild salmon population. The fish were derived from population are used supplement its abundance. Levels of genetic variation similar groups at 11 microsatellite loci, exclusion probabilities >0.999999 for both groups. ability unambiguously identify a pair parents each sampled progeny was much lower than expected, however. Simulations demonstrated that proportion cases which most...
Fall-run chinook salmon Oncorhynchus tshawytscha collected during 1999 carcass surveys of seven locations within the Sacramento River basin and six San Joaquin basin, California, were screened using Y-chromosome-specific marker OtY1 to evaluate inconsistencies between sexual genotype phenotype. Of 287 phenotypic females screened, 46 (16% overall) tested positive for Y-chromosome marker. Stream populations had higher frequencies sex-reversed males (American River, 20%; Battle Creek, 35%;...
Fluorescence in situ hybridization (FISH) was used to identify the X and Y chromosomes of offspring produced by normal "apparent" XY-female fall-run Chinook salmon (Oncorhynchus tshawytscha) from California. FISH experiments were performed using probes 2 sex-linked loci, growth hormone pseudogene (GH-Psi), OtY1, as well a probe microsatellite (Omy7INRA). Comparison staining patterns between apparent XY-females revealed that examined transmitted "Y-like" chromosome with an attenuated OtY1...
Single crystal structures of lanthanide carbonate and hydroxy-carbonate compounds have been previously reported in the literature, with majority these being extended one- to three-dimensional compounds. Very few isolated that contain molecular moieties, none for either erbium or dysprosium. crystals tetra-carbonate complexes, [C(NH2)3]5[Er(CO3)4]·11H2O (I) [C(NH2)3]4[Dy(CO3)4(H2O)](H3O)·13H2O (II), were from concentrated guanidinium solutions characterized by single X-ray diffraction...
Abstract The apparent increase in altered sexual differentiation Pacific salmon Oncorhynchus spp. is a growing concern. Previous studies suggest that incongruence between genetic and phenotypic sex Chinook O. tshawytscha may be result of through exposure to endocrine‐disrupting chemicals (EDCs). Artificial crosses genotypically normal salmon, or males sex‐reversed (XY females), were performed test the validity OtY1 growth hormone pseudogene ( GH Ψ) markers as indicators fall‐run salmon....
A direct, mild, and general method for the enantioselective allylsilylation of aldehydes with allyl chlorides is reported. The reactions are effectively catalyzed by 5 mol % n-Bu4NBr, this rate acceleration allows use complex donors in fragment-coupling electron-deficient donors. results (1) significant progress toward a "universal" asymmetric aldehyde allylation reaction that can reliably highly stereoselectively couple any chloride_aldehyde combination (2) discovery novel mode nucleophilic...
Abstract Spongistatin 1 is among the most potent anti-proliferative agents ever discovered rendering it an attractive candidate for development as a payload antibody–drug conjugates and other targeted delivery approaches. Unfortunately, unavailable from natural sources its size complex stereostructure render chemical synthesis highly time- resource-intensive. As result, design of more acid-stable linker functional group-equipped analogs that retain low picomolar potency parent product...
Abstract Many salmonids have a male heterogametic (XX/XY) sex determination system, and they are supposed to conserved master sex-determining gene (sdY) that interacts at the protein level with Foxl2 leading blockage of synergistic induction Nr5a1 cyp19a1a promoter. However, this hypothesis role sdY in is challenged by few exceptions, one them being presence naturally occurring “apparent” XY Chinook salmon, Oncorhynchus tshawytscha, females. Here, we show some salmon females (sdY-N183), 1...
Abstract Mitochondrial DNA (mtDNA) haplotype diversity between putative XY females and genetically normal of fall‐ spring‐run Chinook salmon Oncorhynchus tshawytscha in California's Central Valley were compared to ascertain whether or not a subset mtDNA haplotypes are unique females. Two Y‐chromosome markers, OtY1 growth hormone pseudogene ( GH ‐Ψ), used screen spring collected on Butte, Deer, Mill creeks 2005 for the presence Fall‐run fish from 2002 2004 had previously been screened...
ABSTRACT Many salmonids have a male heterogametic (XX/XY) sex determination system, and they are supposed to conserved master determining gene ( sdY ), that interacts at the protein level with Foxl2 leading blockage of synergistic induction Nr5a1 cyp19a1a promoter. However, this hypothesis role in is still challenged by few exceptions, one them being presence some naturally occurring “apparent” XY Chinook salmon females. Here we show females sdY-N183 which has missense mutation substitution...
Abstract Review: 221 refs.
Abstract N‐Sulfonyl oxaziridines react with terminal alkenes in the presence of a novel iron(II)/chiral bis(oxazoline) catalyst to give oxazolidines excellent regio‐ and diastereoselectivity (cis:trans <10:1), up 95% enantioselectivity.