A5060866431- Muscle Physiology and Disorders
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- Single-cell and spatial transcriptomics
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Planarian Biology and Electrostimulation
- RNA Research and Splicing
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- High Altitude and Hypoxia
- Ophthalmology and Eye Disorders
- Organ and Tissue Transplantation Research
- Genetic Syndromes and Imprinting
- Extracellular vesicles in disease
- Vestibular and auditory disorders
Centre National de la Recherche Scientifique
2013-2024
Institut Pasteur
2013-2024
Université Paris Cité
2023
Institut Curie
2018-2020
Dynamique du noyau
2018-2020
Laboratoire de Biologie du Développement
2016
Institut de Génomique Fonctionnelle
2015
Université de Montpellier
2015
Inserm
2015
Biologie du Développement et Cellules Souches
2013
Abstract Age-related tissue alterations have been associated with a decline in stem cell number and function. Although increased cell-to-cell variability transcription or epigenetic marks has proposed to be major hallmark of ageing, little is known about the molecular diversity cells during ageing. Here we present single multi-omics study mouse muscle cells, combining single-cell transcriptome DNA methylome profiling. Aged show global increase uncoordinated transcriptional heterogeneity...
Genomic imprinting is an epigenetic mechanism that restrains the expression of ∼100 eutherian genes in a parent-of-origin-specific manner. The reason for this selective targeting with seemingly disparate molecular functions unclear. In present work, we show imprinted are coexpressed network regulated at transition from proliferation to quiescence and differentiation during fibroblast cell cycle withdrawal, adipogenesis vitro, muscle regeneration vivo. Imprinted gene regulation not linked...
Stem cells can be maintained through symmetric cell divisions (SCDs) and asymmetric (ACDs). How when these occur in vivo vertebrates is poorly understood. Here, we developed a clonogenic tracing method that demonstrates the distribution of transcription factors along with old new DNA mouse muscle stem during skeletal regeneration. Combining single-cell tracking artificial niches ex vivo, show how switch from ACDs to SCDs, suggesting they are not engaged an obligate mode division. Further,...
ABSTRACT Skeletal muscle stem cells (MuSCs) are recognised as functionally heterogeneous. Cranial MuSCs reported to have greater proliferative and regenerative capacity when compared with those in the limb. A comprehensive understanding of mechanisms underlying this functional heterogeneity is lacking. Here, we used clonal analysis, live imaging single cell transcriptomic analysis identify crucial features that distinguish extraocular (EOM) from limb populations. MyogeninntdTom reporter...
Dysregulation of stem cell properties is a hallmark many pathologies, but the dynamic behaviour cells in their microenvironment during disease progression remains poorly understood. Using mdx mouse model Duchenne Muscular Dystrophy, we developed innovative live-imaging muscle (MuSCs) vivo, and ex vivo on isolated myofibres. We show that MuSCs have impaired migration precocious differentiation through unbalanced symmetric divisions, driven by p38 PI3K signalling pathways, contrast to p38-only...
Adult skeletal muscles are maintained during homeostasis and regenerated upon injury by muscle stem cells (MuSCs). A heterogeneity in self-renewal, differentiation regeneration properties has been reported for MuSCs based on their anatomical location. Although derived from extraocular (EOM) have a higher regenerative capacity than those limb muscles, the molecular determinants that govern these differences remain undefined. Here we show EOM distinct DNA methylation signatures associated with...
Abstract The muscle stem cell (MuSC) population is recognized as functionally heterogeneous. Cranial cells, which originate from head mesoderm, can have greater proliferative capacity in culture and higher regenerative potential transplantation assays when compared to those the limb. existence of such functional differences phenotypic outputs remain unresolved a comprehensive understanding underlying mechanisms lacking. We addressed this issue using combination clonal analysis, live imaging,...
Age-related tissue alterations have been associated with a decline in stem cell number and function. Although increased cell-to-cell variability transcription or epigenetic marks has proposed to be major hallmark of ageing, little is known about the molecular diversity cells during ageing. Here, by combined single-cell transcriptome DNA methylome profiling mouse muscle cells, we show striking global increase uncoordinated transcriptional heterogeneity together context-dependent methylation...
ABSTRACT Adult skeletal muscles are maintained during homeostasis and regenerated upon injury by muscle stem cells (MuSCs). A heterogeneity in self-renewal, differentiation regeneration properties has been reported for MuSCs based on their anatomical location. Although derived from extraocular (EOM) have a higher regenerative capacity than those limb muscles, the molecular determinants that govern these differences remain undefined. Here we show EOM distinct DNA methylation signatures...
Abstract Stem cells are maintained through symmetric or asymmetric cell divisions. While various mechanisms initiate fates during mitosis, possible epigenetic control of this process has emerged recently. The asymmetrical distribution a canonical histone H3 variant mitosis in fly germline suggested role for partitioning old and new nucleosomes fates. Here, we provide resources single assays show the segregation transcription factors along with DNA mouse muscle stem ex vivo . However, these...