A5021762340- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neonatal Respiratory Health Research
- Pancreatitis Pathology and Treatment
- Lung Cancer Treatments and Mutations
- Ferroptosis and cancer prognosis
- Influenza Virus Research Studies
- Cancer, Lipids, and Metabolism
- Inhalation and Respiratory Drug Delivery
- Optical Imaging and Spectroscopy Techniques
- Antifungal resistance and susceptibility
- Amoebic Infections and Treatments
- Respiratory viral infections research
- Peptidase Inhibition and Analysis
- Cystic Fibrosis Research Advances
- Telomeres, Telomerase, and Senescence
- Vascular Procedures and Complications
- Management of metastatic bone disease
- Skin Diseases and Diabetes
- Respiratory Support and Mechanisms
Université Paris Cité
2015-2024
Institut Pasteur
2014-2024
Centre Hospitalier de la Côte Basque
2021
Centre National de la Recherche Scientifique
2014-2019
Physiopathologie et Epidémiologie des Maladies Respiratoires
2015-2018
Inserm
2015-2018
Délégation Paris 7
2015-2018
Sorbonne Paris Cité
2018
Sorbonne Université
2018
Société Nationale des Chemins de Fer Français (France)
2014
Understanding how SARS-CoV-2 spreads within the respiratory tract is important to define parameters controlling severity of COVID-19. Here we examine functional and structural consequences infection in a reconstructed human bronchial epithelium model. replication causes transient decrease epithelial barrier function disruption tight junctions, though viral particle crossing remains limited. Rather, leads rapid loss ciliary layer, characterized at ultrastructural level by axoneme...
Helicobacter pylori (Hp) is a major risk factor for gastric cancer (GC). Hp promotes DNA damage and proteasomal degradation of p53, the guardian genome stability. reduces expression transcription USF1 shown to stabilise p53 in response genotoxic stress. We investigated whether Hp-mediated deregulation impacts p53-response consequently genetic instability. also explored vivo role carcinogenesis.Human epithelial cell lines were infected with Hp7.13, exposed or not DNA-damaging agent...
Abstract Hepatic fibrosis is a major consequence of chronic liver disease such as non-alcoholic steatohepatitis which undergoing dramatic evolution given the obesity progression worldwide, and has no treatment to date. stellate cells (HSCs) play key role in process, because damage, they transdifferentiate from “quiescent” an “activated” phenotype responsible for most collagen deposition tissue. Here, using diet-induced murine model (choline-deficient amino acid-defined, high fat diet), we...
Abstract The zoonotic bacterium Leptospira interrogans is the aetiological agent of leptospirosis, a re-emerging infectious disease that growing public health concern. Most human cases leptospirosis result from environmental infection. Biofilm formation and its contribution to persistence virulent leptospires in environment or host have scarcely been addressed. Here, we examined spatial time-domain changes biofilm production by L. . Our observations showed highly dynamic process leads...
Dysregulation of stem cell properties is a hallmark many pathologies, but the dynamic behaviour cells in their microenvironment during disease progression remains poorly understood. Using mdx mouse model Duchenne Muscular Dystrophy, we developed innovative live-imaging muscle (MuSCs) vivo, and ex vivo on isolated myofibres. We show that MuSCs have impaired migration precocious differentiation through unbalanced symmetric divisions, driven by p38 PI3K signalling pathways, contrast to p38-only...
Epithelial ovarian cancer cells metastasize by implanting onto the peritoneal mesothelial surface of abdominal cavity. Adhesive molecules that lead to this implantation remain unclear. The aim our study was focus on role vitronectin (Vn) and its receptors, alpha(v) integrins urokinase plasminogen activator receptor (uPAR), in interactions adenocarcinoma (IGROV1 SKOV3 cell lines) with (MeT-5A line primary cultures). For all lines, immunofluorescence staining disclosed presence Vn over whole...
The airway epithelium is the first barrier interacting with Aspergillus fumigatus conidia after their inhalation, suggesting that this structure functions as point of entry fungus to initiate pulmonary aspergillosis. To study epithelial by A fumigatus, primary human reconstituted pseudostratified cultured in air-liquid interface well bronchial cell monolayers were infected conidia. Under these experimental conditions, we found hyphae traversed through a mechanism involving recruitment actin,...
ABSTRACT Understanding how SARS-CoV-2 spreads within the respiratory tract is important to define parameters controlling severity of COVID-19. We examined functional and structural consequences infection in a reconstituted human bronchial epithelium model. replication caused transient decrease epithelial barrier function disruption tight junctions, though viral particle crossing remained limited. Rather, led rapid loss ciliary layer, characterized at ultrastructural level by axoneme...
Gastric cancer represents a major health burden worldwide and is often diagnosed at an advanced stage. Biomarkers for screening prevention of gastric are missing. Changes in peripheral blood mitochondrial DNA (mtDNA) have emerged as potential preventive/diagnosis biomarker risk. We aimed to determine whether leukocytes mtDNA levels associated with stages the carcinogenesis cascade.We measured by quantitative real-time PCR assay 28 patients non-atrophic gastritis (NAG), 74 cancer, 48 matched...
Ovarian carcinomas, the most fatal gynaecological malignancies, are associated with poor prognosis predominantly because of a high recurrence rate. cancer cells spread widely throughout abdominal cavity leading to peritoneal metastasis. The influence mesothelial microenvironment on biological mechanisms cell colonization mesothelium is poorly understood. This study aims investigate whether secretions affect migration ovarian and focuses role adhesive molecule Vn (vitronectin) its integrin...
Targeting mitochondria is a powerful strategy for pathogens to subvert cell physiology and establish infection. Helicobacter pylori bacterial pathogen associated with gastric cancer development that known target directly exclusively through its pro-apoptotic vacuolating cytotoxin VacA. By in vitro infection of epithelial cells wild-type VacA-deficient H. strains, treatment purified VacA proteins mouse model, we show deregulates by two novel mechanisms, both rather host survival. First, early...
Ovarian cancer progression is frequently associated with the development of malignant ascites. Multicellular aggregates carcinoma cells (spheroids) found within ascites are thought to be able promote peritoneal carcinomatosis. We have previously demonstrated involvement vitronectin/alphav integrin adhesive system in dissemination ovarian and continue investigate influence these molecules by studying their role(s) spheroid behavior. The aim this study was generate multicellular focus on role...
Entamoeba histolytica is the causative agent of amebiasis, an infectious disease targeting intestine and liver in humans. Two types intestinal infection are caused by this parasite: silent infection, which occurs majority cases, invasive disease, affects 10% infected persons. To understand pathogenic process, several vitro models, such as cell cultures, human tissue explants or xenografts mice, have been employed. Nevertheless, our knowledge on early steps amebic molecules involved during...
Helicobacter pylori infection causes chronic gastritis and is the major risk factor of gastric cancer. H. induces a inflammation-producing reactive oxygen species (ROS) which source chromosome instabilities contributes to development malignancy. also promotes DNA hypermethylation, known dysregulate essential genes that maintain genetic stability. The maintenance telomere length by telomerase for integrity. Telomerase reverse transcriptase (TERT) catalytic component activity an important...
The life cycle of influenza A viruses (IAV), and notably intracellular trafficking the viral genome, depends on multiple interactions with cellular cytoskeleton endomembrane system. limitation conventional models used for mechanistic study subcellular imaging IAV infection is that they are cultured in two dimensions (2D) under non-polarizing conditions, therefore do not recapitulate organization polarized respiratory epithelial cells naturally targeted by IAVs. To overcome this limitation,...
<div>Abstract<p><b>Background:</b> Gastric cancer represents a major health burden worldwide and is often diagnosed at an advanced stage. Biomarkers for screening prevention of gastric are missing. Changes in peripheral blood mitochondrial DNA (mtDNA) have emerged as potential preventive/diagnosis biomarker risk. We aimed to determine whether leukocytes mtDNA levels associated with stages the carcinogenesis cascade.</p><p><b>Methods:</b>...