A5064116117- Mesenchymal stem cell research
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Heterotopic Ossification and Related Conditions
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Wound Healing and Treatments
- MicroRNA in disease regulation
- Muscle metabolism and nutrition
- Research on Leishmaniasis Studies
- Cervical and Thoracic Myelopathy
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Neurogenetic and Muscular Disorders Research
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- Signaling Pathways in Disease
- Thermoregulation and physiological responses
- Climate Change and Health Impacts
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Plasma Applications and Diagnostics
- RNA and protein synthesis mechanisms
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Advanced biosensing and bioanalysis techniques
Inserm
2016-2024
Université Paris-Saclay
2017-2024
Institut National de la Transfusion Sanguine
2020-2024
Biomedical Research Institute
2024
Hôpital Paul-Brousse
2017-2023
Service de Santé des Armées
2005-2023
Université Paris Cité
2020-2022
Institut de Médecine Tropicale du Service de Santé des Armées
2019-2020
Laboratoire d'études sur les monothéismes
2020
Institute of Rural Management Anand
2020
Myostatin, a member of the TGF-β family, has been identified as master regulator embryonic myogenesis and early postnatal skeletal muscle growth. However, cumulative evidence also suggests that alterations in mass are associated with dysregulation myostatin expression may contribute to loss adulthood. Two major branches Akt pathway relevant for regulation mass, Akt/mammalian target rapamycin (mTOR) pathway, which controls protein synthesis, Akt/forkhead box O (FOXO) degradation. Here, we...
Here, we studied muscle-specific and muscle-related miRNAs in plasma of exercising humans. Our aim was to determine whether they are affected by eccentric and/or concentric exercise modes could be biomarkers muscle injuries or possible signaling molecules. On two separate days, nine healthy subjects randomly performed 30-min walking exercises, one downhill (high component) uphill component). Perceived exertion heart rate were higher during the exercise, while subjective pain ankle plantar...
Neurogenic heterotopic ossification (NHO) is the formation of ectopic bone generally in muscles surrounding joints following spinal cord or brain injury. We investigated mechanisms NHO 64 patients and a mouse model injury–induced NHO. show that marrow from human NHOs contains hematopoietic stem cell (HSC) niches, which mesenchymal stromal cells (MSCs) endothelial provide an environment supporting HSC maintenance, proliferation, differentiation. The transcriptomic signature MSCs shows...
Abstract Treatment with cold atmospheric plasma (CAP) has been reported to promote wound healing in animals. However, how this process is mediated remains unclear. In study we examined the mechanisms which underlie improved effects of CAP and roles associated reactive oxygen nitrogen species (RONS), are generated by plasma. By using vitro models mimicked various steps angiogenesis, demonstrated that triggered production nitric oxide (NO), enhanced cell migration assembly endothelial cells...
Myostatin is a master regulator of myogenesis and early postnatal skeletal muscle growth. However, myostatin has been also involved in several forms wasting adulthood, suggesting functional role for the regulation mass adult. In present study, localized ectopic expression was achieved by gene electrotransfer vector into tibialis anterior adult Sprague Dawley male rats. The corresponding empty electrotransfected contralateral muscle. Ectopic mRNA abundantly muscles with vector, whereas it...
In this study, we quantified the transcription of interleukin‐6 (IL‐6) gene in individual fibres and associated changes calcineurin activity assessed at cellular level during prolonged muscle contraction. Individual myofibres were isolated from plantaris soleus muscles rats end an exhaustive running exercise test ( n = 10), categorized according to their myosin heavy chain isoform content, compared those resting 10). Using real‐time PCR analysis fibres, a marked rise IL‐6 transcript levels...
The present study was designed to test the hypothesis that increasing physical activity by running exercise could favor recovery of muscle mass after extensive injury and determine main molecular mechanisms involved. Left soleus muscles female Wistar rats were degenerated notexin injection before animals assigned either a sedentary group or an exercised group. Both regenerating contralateral intact from active removed 5, 7, 14, 21, 28 42 days (n = 8 rats/group). Increasing contractile...
Prolonged intense exercise is challenging for the liver to maintain plasma glucose levels. Hormonal changes cannot fully account exercise-induced hepatic production (HGP). Contracting skeletal muscles release interleukin-6 (IL-6), a cytokine able increase endogenous during exercise. However, whether this attributable direct effect of IL-6 on remains unknown. Here, we studied glycogen, gluconeogenic genes, and signaling in response one bout exhaustive running rats. To determine can modulate...
Mesenchymal stromal cell (MSC)-based therapy has received great interest in regenerative medicine. Priming the cells during culture phase can improve their efficacy and/or survival after injection. The literature suggests that MSC extracellular vesicles (EV) recapitulate a substantial part of beneficial effects they originate from, and micro-RNAs (miRNAs) are important players EV biological action. Here, our aim was to determine if two classical priming methods MSC, interferon-gamma (IFNγ)...
Abstract Skin grafting is a surgical method of cutaneous reconstruction, which provides volumetric replacement in wounds unable to heal by primary intention. Clinically, full‐thickness skin grafts (FTSGs) are placed aesthetically sensitive and mechanically demanding areas such as the hands, face, neck. Complete or partial graft failure complication associated with this procedure. Strategies aimed at improving rate integration will reduce incidence failure. Cold atmospheric plasma (CAP) an...
ABSTRACT Neurogenic heterotopic ossifications (NHOs) form in periarticular muscles after severe spinal cord (SCI) and traumatic brain injuries. The pathogenesis of NHO is poorly understood with no effective preventive treatment. only curative treatment remains surgical resection pathological NHOs. In a mouse model SCI-induced that involves transection the combined muscle injury, differential gene expression analysis revealed genes involved inflammation such as interleukin-1β (IL-1β) were...
Abstract Background Cardiovascular diseases are the main cause of morbidity and mortality worldwide. Restoring blood supply to ischemic tissues is an essential goal for successful treatment these diseases. Growth factor or gene therapy efficacy remains controversial, but stem cell transplantation emerging as interesting approach stimulate angiogenesis. Among different populations, cord blood-endothelial progenitor cells (CB-EPCs) more particularly cell-derived endothelial colony forming...
The cells of origin neurogenic heterotopic ossifications (NHOs), which develop frequently in the periarticular muscles following spinal cord injuries (SCIs) and traumatic brain injuries, remain unclear because skeletal muscle harbors two progenitor cell populations: satellite (SCs), are myogenic, fibroadipogenic progenitors (FAPs), mesenchymal. Lineage-tracing experiments using Cre recombinase/LoxP system were performed mouse strains with fluorescent protein ZsGreen specifically expressed...
Despite several advances in the field of regenerative medicine, clinical management extensive skin wounds or burns remains a major therapeutic issue. During past few years, Mesenchymal Stromal Cells (MSCs) have emerged as novel tool to promote tissue repair through their anti-inflammatory, pro-trophic and pro-remodeling effects. They exert biological activity mainly via secretion soluble bioactive molecules such cytokines, growth factors, proteins microRNAs which can be encapsulated within...
Abstract Extracellular matrices (ECM) rich in type I collagen exhibit characteristic anisotropic ultrastructures. Nevertheless, working vitro with this biomacromolecule remains challenging. When processed, denaturation of the molecule is easily induced avoiding proper fibril self‐assembly and further hierarchical order. Here, an innovative approach enables production highly concentrated injectable microparticles, based on molecules self‐assembly, thanks to use spray‐drying process. The...
Staphylococcus aureus is the most frequent cause of skin and soft tissue infections. Treatment failures are increasingly common due to antibiotic resistance emergence resistant strains.
Abstract The present work aimed at determining whether interleukin‐6 (IL‐6) produced by skeletal muscle during exercise is related, least partly, to calcineurin activity. Rats were treated with two specific inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they then subjected exhaustive treadmill running. Modulatory Calcineurin‐Interacting Protein‐1 (MCIP‐1) mRNA levels, a reliable indicator of activity, IL‐6 levels measured real‐time RT‐PCR in soleus muscles, protein...