A5048809255- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Neuroblastoma Research and Treatments
- Adenosine and Purinergic Signaling
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Heterotopic Ossification and Related Conditions
- Cancer Cells and Metastasis
- High Altitude and Hypoxia
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related Molecular Pathways
- Quinazolinone synthesis and applications
- Cancer Mechanisms and Therapy
- Bone fractures and treatments
- Pneumocystis jirovecii pneumonia detection and treatment
The University of Queensland
2014-2022
Translational Research Institute
2012-2022
QIMR Berghofer Medical Research Institute
2020-2022
Queensland University of Technology
2017-2022
Mater Research
2011-2021
Prostate Cancer Research
2019
Griffith University
2006
Abstract Despite the fact that many hypoxia-inducible genes are important in hematopoiesis, spatial distribution of oxygen bone marrow (BM) has not previously been explored vivo. Using hypoxia bioprobe pimonidazole, we showed by confocal laser scanning microscopy endosteum at bone-BM interface is hypoxic, with constitutive expression transcription factor-1α (HIF-1α) protein steady-state mice. Interestingly, peak hematopoietic stem and progenitor cell (HSPC) mobilization induced either...
Traumatic spinal cord injury (SCI) triggers an acute-phase response that leads to systemic inflammation and rapid mobilization of bone marrow (BM) neutrophils into the blood. These mobilized then accumulate in visceral organs injured where they cause inflammatory tissue damage. The receptor for complement activation product 3a, C3aR1, has been implicated negatively regulating BM neutrophil injury. However, mechanism via which C3aR1 controls mobilization, also its influence over SCI outcomes,...
Natural killer (NK) cell protection from tumor metastases is a critical feature of the host immune response to cancer, but various immunosuppression mechanisms limit NK effector function. The ectoenzyme, CD39, expressed on tumor-infiltrating myeloid cells, granulocytes, and lymphocytes, including converts extracellular ATP (eATP) into AMP and, thus, potentially suppresses eATP-mediated proinflammatory responses. A CD39-targeting monoclonal antibody (mAb) that inhibits mouse ectoenzyme CD39...
Tumor antigen-specific CD8+ T cells play a critical role in antitumor immunity. Clinical trials reinvigorating the immune system via checkpoint blockade (ICB) have shown remarkable clinical promise. Numerous studies identified an association between NKG7 expression and patient outcome across different malignancies. However, aside from these correlative observations, very little is known about its Herein, we utilized single-cell RNA sequencing (scRNA-seq) datasets, NKG7-deficient mice,...
Many patients with hematological neoplasms fail to mobilize sufficient numbers of hematopoietic stem cells (HSCs) in response granulocyte colony-stimulating factor (G-CSF) precluding subsequent autologous HSC transplantation. Plerixafor, a specific antagonist the chemokine receptor CXCR4, can rescue some but not all who failed G-CSF alone. These refractory poor mobilizers cannot currently benefit from To discover alternative targetable pathways enhance mobilization, we studied role...
Osteoblasts are necessary to B lymphopoiesis and mobilizing doses of G-CSF or cyclophosphamide inhibit osteoblasts, whereas AMD3100/Plerixafor does not. However, the effect these agents on has not been reported. Mice (wild-type, knocked-out for TNF-α TRAIL, over-expressing Bcl-2) were mobilized with G-CSF, cyclophosphamide, AMD3100. Bone marrow, blood, spleen lymph node content in cells was measured. stopped medullar concomitant loss B-cell colony-forming units, pre-pro-B, pro-B, pre-B...
A strictly aerobic, rod-shaped bacterium (0.6-0.8 x2-3 microm), designated strain Kh10-101T, was isolated from a saltpan (22 degrees 15' N, 69 1' E) in the vicinity of Port Okha, India. The creamish pigmented colonies Kh10-101T were round, flat and translucent with irregular margins smooth surface. possessed up to three subpolar flagella, motile by corkscrew motion. grew optimally at 37 C (temperature growth range 25-40 C) complex glucose-containing medium 5 % NaCl (NaCl 0-10 %) pH 9 (pH...
The cells of origin neurogenic heterotopic ossifications (NHOs), which develop frequently in the periarticular muscles following spinal cord injuries (SCIs) and traumatic brain injuries, remain unclear because skeletal muscle harbors two progenitor cell populations: satellite (SCs), are myogenic, fibroadipogenic progenitors (FAPs), mesenchymal. Lineage-tracing experiments using Cre recombinase/LoxP system were performed mouse strains with fluorescent protein ZsGreen specifically expressed...
In normoxia, hypoxia-inducible transcription factors (HIFs) are rapidly degraded within the cytoplasm as a consequence of their prolyl hydroxylation by oxygen-dependent hydroxylase domain (PHD) enzymes. We have previously shown that hematopoietic stem and progenitor cells (HSPCs) require HIF-1 for effective mobilization in response to granulocyte colony-stimulating factor (G-CSF) CXCR4 antagonist AMD3100/plerixafor. Conversely, HIF PHD inhibitors stabilize protein vivo enhance HSPC G-CSF or...
Anemia of inflammation (AI) is the second most prevalent anemia after iron deficiency and results in persistent low blood erythrocytes hemoglobin, fatigue, weakness early death. AI common people with chronic inflammation, infections or sepsis. Although several studies have reported effect on stress erythropoiesis homeostasis, mechanisms by which suppresses bone marrow, where differentiation maturation erythroid cells from hematopoietic stem occurs, not been extensively studied. Here we show...
Staining for CD27 and CD201 (endothelial protein C receptor) has been recently suggested as an alternative to stem cell antigen–1 (Sca1) identify hematopoietic cells in inbred mouse strains with low or nil expression of SCA1. However, whether staining is compatible fms-like tyrosine kinase 3 (FLT3) the "SLAM" code defined by CD48 CD150 long-term reconstituting not established. We compared C57BL/6 strain, which expresses a high level SCA1 on non-obese diabetic severe combined immune deficient...