A5080139485- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Platelet Disorders and Treatments
- Proteoglycans and glycosaminoglycans research
- Acute Myeloid Leukemia Research
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer Cells and Metastasis
- Bone and Dental Protein Studies
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Virus-based gene therapy research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Neonatal Respiratory Health Research
- Blood disorders and treatments
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
Monash University
2015-2024
Health Sciences and Nutrition
2012-2024
Australian Regenerative Medicine Institute
2015-2024
Commonwealth Scientific and Industrial Research Organisation
2012-2023
CSIRO Manufacturing
2011-2021
Clayton Foundation
2017
Physical Sciences (United States)
2016
The Ohio State University
2016
The University of Melbourne
2006-2014
Materials Science & Engineering
2010-2014
Originally identified as a marker specifying murine hematopoietic stem cells, the Sca-1 antigen has since been shown to be differentially expressed by candidate cells in tissues including vascular endothelium, skeletal muscle, mammary gland, and prostate of adult mice. In lung, previously selectable for isolation nonhematopoietic (CD45(-)), nonendothelial (CD31(-)) bronchioalveolar (BASC) located at duct junction that coexpress surfactant protein C Clara cell specific protein. Our systematic...
Abstract Despite the fact that many hypoxia-inducible genes are important in hematopoiesis, spatial distribution of oxygen bone marrow (BM) has not previously been explored vivo. Using hypoxia bioprobe pimonidazole, we showed by confocal laser scanning microscopy endosteum at bone-BM interface is hypoxic, with constitutive expression transcription factor-1α (HIF-1α) protein steady-state mice. Interestingly, peak hematopoietic stem and progenitor cell (HSPC) mobilization induced either...
Abstract Pharmaceutical and agrochemical discovery programs are under considerable pressure to meet increasing global demand thus require constant innovation. Classical hydrocarbon scaffolds have long assisted in bringing new molecules the market place, but an obvious omission is that of Platonic solid cubane. Eaton, however, suggested this molecule has potential act as a benzene bioisostere. Herein, we report validation Eaton's hypothesis with cubane derivatives five used clinically or...
Hematopoiesis is a multistage process involving the differentiation of stem and progenitor cells into distinct mature cell lineages. Here we present Haemopedia, an atlas murine gene-expression data containing 54 hematopoietic types, covering all lineages in hematopoiesis. We include rare populations such as eosinophils, mast cells, basophils, megakaryocytes, broad collection cells. show that lineage branching maturation during hematopoiesis can be reconstructed using expression patterns...
Abstract With age, hematopoietic stem cells (HSC) undergo changes in function, including reduced regenerative potential and loss of quiescence, which is accompanied by a significant expansion the cell pool that can lead to haematological disorders. Elevated metabolic activity has been implicated driving HSC ageing phenotype. Here we show nicotinamide riboside (NR), form vitamin B3, restores youthful capacity modifying mitochondrial function multiple ways expression nuclear encoded pathway...
A mechanistic connection between aging and development is largely unexplored. Through profiling age-related chromatin transcriptional changes across 22 murine cell types, analyzed alongside previous mouse human organismal maturation datasets, we uncovered a transcription factor binding site (TFBS) signature common to both processes. Early-life candidate cis-regulatory elements (cCREs), progressively losing accessibility during aging, are enriched for cell-type identity TFBSs. Conversely,...
The mechanism of hemopoietic stem cell homing to the bone marrow involves molecular interactions that mediate recognition and interaction these cells with microenvironment, including extracellular matrix. On selective binding, this environment, in combination soluble cytokines, regulates proliferation differentiation. Using immunofluorescence labeling, we analyzed location prominent matrix proteins fibronectin, collagen Types I, III, IV, laminin sections murine femoral marrow. Collagen...
Allogeneic and autologous marrow transplants are routinely used to correct a wide variety of diseases. In addition, potentially provide opportune means delivering genes in transfected, engrafting stem cells. However, relatively little is known about the mechanisms engraftment transplant recipients, especially nonablated setting with regard cells not hemopoietic origin. particular, this includes stromal progenitors osteoblastic lineage. We have demonstrated for first time that whole bone...