Vincent Tano
A5068961956- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Genetic Neurodegenerative Diseases
- Genomics and Phylogenetic Studies
- MicroRNA in disease regulation
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- Infant Nutrition and Health
- Genetics and Neurodevelopmental Disorders
- Liver Diseases and Immunity
- Ferroptosis and cancer prognosis
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
Nanyang Technological University
2020-2024
National University Cancer Institute, Singapore
2024
National University of Singapore
2024
Monash University
2020
The University of Melbourne
2018-2019
Hepatocellular carcinoma (HCC) tumour microenvironment (TME) is highly complex with diverse cellular components organising into various functional units, neighbourhoods (CNs). And we wanted to define CN of HCC while preserving the TME architecture, based on which, potential targets for novel immunotherapy could be identified.A multiplexed imaging mass cytometry (IMC) panel was designed simultaneously quantify 36 biomarkers tissues from 134 patients and 7 healthy donors generate 562 histology...
Abstract Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in training cohort (n =...
Colorectal cancer (CRC) is a common malignancy involving multiple cellular components. The CRC tumor microenvironment (TME) has been characterized well at single-cell resolution. However, spatial interaction map of the TME still elusive. Here, we integrate multiomics analyses and establish to improve prognosis, prediction, therapeutic development for CRC. We construct immune module (CCIM) that comprises FOLR2+ macrophages, exhausted CD8+ T cells, tolerant CD4+ regulatory cells. Multiplex...
In Huntington's disease (HD), a CAG repeat expansion mutation in the Huntingtin (HTT) gene drives gain-of-function toxicity that disrupts mRNA processing. Although dysregulation of splicing has been shown human HD post-mortem brain tissue, analyses are likely confounded by cell type composition changes late-stage HD, limiting ability to identify related early pathogenesis.To investigate we performed alternative coupled with proteogenomics approach length-associated an established isogenic...
Mitchell-Riley syndrome (MRS) is caused by recessive mutations in the regulatory factor X6 gene (RFX6) and characterised pancreatic hypoplasia neonatal diabetes. To determine why individuals with MRS specifically lack endocrine cells, we micro-CT imaged a 12-week-old foetus homozygous for nonsense mutation RFX6 c.1129C>T, which revealed loss of pancreas body tail. From this foetus, derived iPSCs show that differentiation these cells vitro proceeds normally until generation endoderm,...
N 6 -methyladenosine (m A) RNA methylation is a prevalent modification that significantly impacts metabolism and cancer development. Maintaining the global m A levels in cells relies on accessibility to methyltransferases availability of methyl donor S-adenosylmethionine (SAM). Here, we reveal death associated protein 3 (DAP3) plays crucial role preserving through two distinct mechanisms. First, although DAP3 not component writer complex, it directly binds target regions, thereby...
A strong focus of the post-genomic era is mining non-coding regulatory genome in order to unravel function elements that coordinate gene expression (Nat 489:57–74, 2012; Nat 507:462–70, 2014; 507:455–61, 518:317–30, 2015). Whole-genome approaches based on next-generation sequencing (NGS) have provided insight into genomic location throughout different cell types, organs and organisms. These technologies are now widespread commonly used laboratories from various fields research. This...
Abstract Expansion of the CAG trinucleotide repeat tract in exon 1 Huntingtin ( HTT ) gene above a threshold ∼36 repeats causes Huntington’s disease (HD) through expression polyglutamine-expanded form protein. This mutation triggers wide-ranging cellular and biochemical pathologies leading to cognitive, motor, psychiatric symptoms HD patients. As accurate splicing is required produce full-length protein ∼348 kDa, targeting with small molecule drugs compelling approach lower levels treat HD,...
ABSTRACT In Huntington’s disease (HD), a CAG repeat expansion mutation in the HTT gene drives gain-of-function toxicity that disrupts mRNA processing. Although widespread dysregulation of splicing striatum has been shown human HD post-mortem brain tissue, analyses are likely confounded by cell type composition changes due to neuronal loss and astrogliosis late stage HD. This limits ability identify related early pathogenesis. To study alternative HD, we performed RNA-sequencing analysis an...
<div>Abstract<p>Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in...
<div>Abstract<p>Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in...
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis