A5037347081- Fetal and Pediatric Neurological Disorders
- Autopsy Techniques and Outcomes
- Prenatal Screening and Diagnostics
- Congenital Diaphragmatic Hernia Studies
- Reproductive Health and Technologies
- Pregnancy and preeclampsia studies
- Advanced Neuroimaging Techniques and Applications
- Grief, Bereavement, and Mental Health
- Congenital Anomalies and Fetal Surgery
- Urological Disorders and Treatments
- Forensic Anthropology and Bioarchaeology Studies
- Anatomy and Medical Technology
- Patient Safety and Medication Errors
- Histone Deacetylase Inhibitors Research
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- MRI in cancer diagnosis
- Cell Image Analysis Techniques
- Medicine and Dermatology Studies History
- Abdominal Trauma and Injuries
- Pancreatic function and diabetes
- Brain Metastases and Treatment
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
Amsterdam University Medical Centers
2019-2024
University Medical Center
2024
University of Amsterdam
2013-2024
Amsterdam UMC Location University of Amsterdam
2013-2018
Abstract Diffusible iodine-based contrast-enhanced computed tomography (diceCT) is progressively used in clinical and morphological research to study developmental anatomy. Lugol’s solution (Lugol) has gained interest as an effective contrast agent; however, usage limited due extensive soft-tissue shrinkage. The mechanism of Lugol-induced shrinkage how prevent it largely unknown, hampering applications Lugol or forensic cases where tissue can lead erroneous diagnostic conclusions. Shrinkage...
Over the last few years, fetal postmortem microfocus computed tomography (micro-CT) imaging has increased in popularity for both diagnostic and research purposes. Micro-CT could be a substitute autopsy, particularly very early gestation fetuses whom autopsy can technically challenging is often unaccepted by parents. This article provides an overview of latest micro-CT with focus on accuracy, endovascular staining approaches, placental studies reversibility staining. It also discusses new...
The decision to terminate a pregnancy due fetal anomalies can have significant emotional impact, especially in second-trimester terminations. Previous studies on the psychological consequences of termination had limitations, and little is known about outcomes for partners impact donation. Therefore, we aimed investigate effects identify factors associated with both women men, including donation remains science.
Mitchell-Riley syndrome (MRS) is caused by recessive mutations in the regulatory factor X6 gene (RFX6) and characterised pancreatic hypoplasia neonatal diabetes. To determine why individuals with MRS specifically lack endocrine cells, we micro-CT imaged a 12-week-old foetus homozygous for nonsense mutation RFX6 c.1129C>T, which revealed loss of pancreas body tail. From this foetus, derived iPSCs show that differentiation these cells vitro proceeds normally until generation endoderm,...
Objectives Down syndrome (DS) is associated with airway abnormalities including a narrowed trachea. It uncertain whether this trachea in DS consequence of deviant fetal development or an acquired disorder following endotracheal intubation after birth. This study aimed to compare the tracheal morphology and non‐DS fetuses using microfocus computed tomography (micro‐CT). Methods Twenty samples were obtained from Dutch Fetal Biobank divided into groups based on gestational age. Micro‐CT images...
Adult spleens show extensive morphological variation, with a reported prevalence of 40-98% clefts (also called notches or fissures) on the splenic surface and 10-30% accessory at autopsy. It is hypothesised that both anatomical variants result from complete partial failure multiple primordia to fuse main body. According this hypothesis, fusion spleen completed after birth variations are often explained as stagnation development foetal stage. We tested hypothesis by studying early in embryos,...
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Although fetal autopsy is generally recommended to confirm or refute the antemortem diagnosis, parental acceptance of procedure has fallen over time, mainly due its invasiveness. Contrast-enhanced microfocus CT (micro-CT) and high-field magnetic resonance imaging (HF-MRI, ≥ 3 Tesla) have both been suggested as non-invasive alternatives conventional for fetuses < 20 weeks gestation. The aim this study was compare these two modalities in postmortem whole-body imaging.In study, process quality...
Objective: The F-box protein Fbxw8 is a cofactor of Cullin 7 (Cul7), which regulates transfer to the proteasome and cell growth. Cul7 or deficiency associated with intrauterine growth restriction (IUGR) due abnormal placental development leading poor oxygen supply fetus. We studied role hypoxia for expression in trophoblastic cells. Methods: Immunomagnetic bead-separated extravillous trophoblast (EVT) villous (VT) lines were incubated 1 8% O2. was determined IUGR versus matched control...
Microfocus Computed Tomography (Micro-CT) is a novel method for non-destructive 3D imaging of samples, reaching microscale resolutions. While initially prominent in material sciences small micro-CT now gains significance biological and medical studies. Here we present our utilization large ex-vivo human samples anatomical forensic research three recent experiments discuss the fundamentals imaging. For pelvic research, whole pelvises were imaged to explore nerve anatomy around prostate using...
Microfocus Computed Tomography (Micro-CT) is a novel method for non-destructive 3D reaching microscale resolutions. We present our utilization of micro-CT imaging large ex-vivo human samples (up to 30 cm) anatomical and forensic research in three experiments. discuss the fundamentals imaging, challenges arising with solutions such as partial destaining improve image quality. Micro-CT exciting technique that can reveal realm this field.
Human fetal tissue is essential for biomedical research, providing unparalleled insights into human development and disease.
This study aimed to assess the feasibility of postmortem ultra-high-field magnetic resonance imaging (UHF-MRI) fetal musculoskeletal anatomy and explore contribution variation in iodine formaldehyde (paraformaldehyde, PFA) treatment tissue.Seven upper extremities from human fetuses with gestational ages 19 24 weeks were included this experimental study, approved by Medical Research Ethics Committee. The specimens treated various storage (0.2-4% staining (Lugol's solution) protocols wrist...
Conclusions: Face to face confirmation of ultrasound scans by expert sonologists yields a higher accuracy though nonstatistically significant difference compared teleultrasound.Teleultrasound is an acceptable alternative when there emergent need for and can limit exposures during pandemic.
To assess the visualisation of postmortem (PM) fetal anatomy in stored specimens with two-dimensional (2D) and three-dimensional (3D) ultrasound (US) ascertain if 3DUS rendering can be used to obtain reference images for use clinical practice. 20 formalin-fixed fetuses (10-24 weeks gestation; research museum collections) were scanned a degassed water bath mimic utero US. Following 2DUS (excluding heart), 3D volumes acquired interrogated Crystal Vue Realistic Vue™ software (Samsung WS80A...
Although images of the brain can be obtained in early pregnancy, interpretation is hampered by an appropriate reference standard. We aim to validate three-dimensional (3D) ultrasonographic appearances ventricular system (VS) from 8-11 weeks using embryological expertise and 3D models (3D Atlas Human Embryology) as a gold Weekly ultrasound (US) examinations were performed on 14 patients gestation. Following routine two-dimensional (2D) US, volumes acquired interrogated with Crystal Vue...