A5029285347- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Esophageal and GI Pathology
- Anesthesia and Neurotoxicity Research
- Intensive Care Unit Cognitive Disorders
- MicroRNA in disease regulation
- Congenital Diaphragmatic Hernia Studies
- Anesthesia and Sedative Agents
- Advanced Photocatalysis Techniques
- Congenital heart defects research
- Nanomaterials for catalytic reactions
- Esophageal Cancer Research and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Ferroptosis and cancer prognosis
- Diet and metabolism studies
- Hippo pathway signaling and YAP/TAZ
- Metabolism, Diabetes, and Cancer
Children's Hospital of Zhejiang University
2018-2025
Zhejiang University
2024
Zhejiang International Studies University
2024
Sichuan University
2024
Nanjing Maternity and Child Health Care Hospital
2024
Nanjing Medical University
2024
China-US (Henan) Hormel Cancer Institute
2023
Mudanjiang Normal University
2023
East China University of Science and Technology
2023
Columbia University Irving Medical Center
2017-2021
Activating mutations in the EGF receptor (EGFR) are associated with clinical responsiveness to EGFR tyrosine kinase inhibitors (TKI), such as erlotinib and gefitinib. However, resistance eventually arises, often due a second mutation, most commonly T790M. Through genome-wide siRNA screen human lung cancer cell line analyses of murine mutant EGFR-driven adenocarcinomas, we found that was reduced expression neurofibromin, RAS GTPase-activating protein encoded by NF1 gene. Erlotinib failed...
OBJECTIVE To explore the relationship between gasdermin D (GSDMD) and gastric cancer (GC) cell proliferation, to determine whether downregulated expression of GSDMD contributed tumorigenesis proliferation GC cells. METHODS expressions in tissues matched adjacent non‐cancerous were assessed by quantitative real‐time polymerase chain reaction, Western blot immunohistochemistry. The effect on vitro was colony formation assay viability assays. In vivo , xenografted tumors nude mice evaluated....
Tissue homeostasis requires balanced self-renewal and differentiation of stem/progenitor cells, especially in tissues that are constantly replenished like the esophagus. Disruption this balance is associated with pathological conditions, including eosinophilic esophagitis (EoE), which basal progenitor cells become hyperplastic upon proinflammatory stimulation. However, how respond to inflammatory environment at molecular level remains undetermined. We previously reported bone morphogenetic...
Abstract Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in training cohort (n =...
Abstract Esophageal squamous precancerous lesions (ESPL) are the precursors of esophageal cell carcinoma (ESCC) including low-grade and high-grade intraepithelial neoplasia. Due to absence molecular indicators, which ESPL will eventually develop into ESCC thus should be treated is not well defined. Indicators, for predicting risks at stages, an urgent need. We perform spatial whole-transcriptome atlas analysis, can eliminate other tissue interference by sequencing specific regions. In this...
CARM1 is a cancer-relevant protein arginine methyltransferase that regulates many aspects of transcription. Its pharmacological inhibition promising anti-cancer strategy. Here SKI-73 (6a in this work) presented as chemical probe with pro-drug properties. (6a) can rapidly penetrate cell membranes and then be processed into active inhibitors, which are retained intracellularly 10-fold enrichment for several days. These compounds were characterized their potency, selectivity, modes action,...
Viral infection often causes severe damage to the lungs, leading appearance of ectopic basal cells (EBCs) and tuft in lung parenchyma. Thus far, roles these epithelial alveolar regeneration remain controversial. Here, we confirm that are originated from EBCs mouse models COVID-19 lungs. The differentiation is promoted by Wnt inhibition while suppressed Notch inhibition. Although progenitor functions have been suggested other organs, pulmonary don’t proliferate or give rise cell lineages....
Mitogen-activated protein kinases (MAPKs) are inactivated by dual-specificity phosphatases (DUSPs), the activities of which tightly regulated during cell differentiation.Using knockdown screening and single-cell transcriptional analysis, we demonstrate that DUSP4 is phosphatase specifically inactivates p38 kinase to promote megakaryocyte (Mk) differentiation.Mechanistically, PRMT1-mediated methylation triggers its ubiquitinylation an E3 ligase HUWE1.Interestingly, mechanistic axis...
Establishment of the functional pulmonary vasculature requires intimate interaction between epithelium and mesenchyme. Previous genetic studies have led to inconsistent conclusions about contribution epithelial Wnts development. This discrepancy is possibly due redundancy among different Wnts. Here, we use Shh-Cre conditionally delete Gpr177 (the mouse ortholog Drosophila Wntless, Wls), a chaperon protein important for sorting secretion Wnt proteins. Deletion reduces signaling activity in...
Recent identification and isolation of suture stem cells capable long-term self-renewal, clonal expanding, differentiating demonstrate their essential role in calvarial bone development, homeostasis, injury repair. These bona fide express a high level Axin2 are able to mediate regeneration repair cell autonomous fashion. The importance is further demonstrated by its genetic inactivation mice causing skeletal deformities resembling craniosynostosis humans. fate determination subsequent...
Basal progenitor cells are crucial for the establishment and maintenance of tracheal epithelium. However, it remains unclear how these specified during foregut development. Here, we found that ablation Wnt chaperone protein Gpr177 (also known as Wntless) in mouse epithelium causes a significant reduction number basal accompanied by cartilage loss Shh-Cre;Gpr177loxp/loxp mutants. Consistent with association between cell development, Nkx2.1+p63+ co-present nodules Shh-Cre;Ctnnb1DM/loxp...
Despite the epidemiological association between intrahepatic cholangiocarcinoma (ICC) and hepatitis B virus (HBV) infection, little is known about relevant oncogenic effects. A cohort of 32 HBV-infected ICC 89 non-HBV-ICC patients were characterized using whole-exome sequencing, proteomic analysis, single-cell RNA sequencing. Proteomic analysis revealed decreased cell-cell junction levels in HBV-ICC patients. The level had an inverse relationship with epithelial-mesenchymal transition (EMT)...
Balanced progenitor activities are crucial for the development and maintenance of high turn-over organs such as esophagus. However, molecular mechanisms regulating these in esophagus remain to be elucidated. Here, we demonstrated that Yap is required proliferation esophageal cells (EPCs) developing murine We found deficiency reduces EPC stratification whereas persistent activation increases cell causes aberrant further role YAP signaling conserved human by utilizing 3D pluripotent stem...
Abstract Objectives The effects of general anaesthetics on fetal brain development remain elusive. Radial glial progenitors (RGPs) generate the majority neurons in developing brains. Here, we evaluated acute alterations RGPs after maternal sevoflurane exposure. Methods Pregnant mice were exposed to 2.5% for 6 hours gestational day 14.5. Interkinetic nuclear migration (INM) ventricular zone (VZ) was by thymidine analogues labelling. Cell fate RGP progeny determined immunostaining using...
The role of peptidoglycan-associated lipoprotein (Pal) in A. baumannii pathogenesis remains unclear. Here, we illustrated its by constructing a pal deficient mutant and complementary strain.Transcriptome analysis the WT revealed total 596 differentially expressed genes. Gene Ontology that deficiency caused downregulation genes related to material transport metabolic processes. showed slower growth was sensitive detergent serum killing compared strain, whereas, complemented rescued phenotype....