A5102762782- Immune cells in cancer
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Inflammation biomarkers and pathways
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related gene regulation
- Gene expression and cancer classification
- Redox biology and oxidative stress
- Psoriasis: Treatment and Pathogenesis
- CRISPR and Genetic Engineering
- Dermatology and Skin Diseases
- Hemoglobinopathies and Related Disorders
- Bioinformatics and Genomic Networks
- Bone health and treatments
- Ferroptosis and cancer prognosis
- Photodynamic Therapy Research Studies
- Genetic Associations and Epidemiology
- Histone Deacetylase Inhibitors Research
- Skin Protection and Aging
- Bone and Joint Diseases
- Cell Image Analysis Techniques
- Marine Biology and Environmental Chemistry
First Affiliated Hospital of Fujian Medical University
2019-2025
Fujian Medical University
2019-2025
Cornell University
2017-2024
Weill Cornell Medicine
2022-2024
Jiangxi University of Traditional Chinese Medicine
2023-2024
New York Genome Center
2017-2023
Tri-Institutional PhD Program in Chemical Biology
2017-2022
Memorial Sloan Kettering Cancer Center
2019
New York University
2017-2019
Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine
2017
Atherosclerosis is a leading cause of death worldwide in industrialized countries. Disease progression and regression are associated with different activation states macrophages derived from inflammatory monocytes entering the plaques. The features monocyte-to-macrophage transition full spectrum macrophage during either plaque or regression, however, incompletely established. Here, we use combination single-cell RNA sequencing genetic fate mapping to profile, for first time our knowledge,...
The hemizygous R47H variant of triggering receptor expressed on myeloid cells 2 (TREM2), a microglia-specific gene in the brain, increases risk for late-onset Alzheimer’s disease (AD). Using transcriptomic analysis single nuclei from brain tissues patients with AD carrying mutation or common (CV)–TREM2, we found that R47H-associated microglial subpopulations had enhanced inflammatory signatures reminiscent previously identified disease-associated microglia (DAM) and hyperactivation AKT, one...
Unlike sequencing-based methods, which require cell lysis, optical pooled genetic screens enable investigation of spatial phenotypes, including morphology, protein subcellular localization, cell–cell interactions and tissue organization, in response to targeted CRISPR perturbations. Here we report a multimodal screening method, call CRISPRmap. CRISPRmap combines situ guide-identifying barcode readout with multiplexed immunofluorescence RNA detection. Barcodes are detected read out through...
CARM1 is a cancer-relevant protein arginine methyltransferase that regulates many aspects of transcription. Its pharmacological inhibition promising anti-cancer strategy. Here SKI-73 (6a in this work) presented as chemical probe with pro-drug properties. (6a) can rapidly penetrate cell membranes and then be processed into active inhibitors, which are retained intracellularly 10-fold enrichment for several days. These compounds were characterized their potency, selectivity, modes action,...
Abstract Immunotherapies are a transformative force in clinical oncology, delivering prolonged disease-free survival refractory cancers. However, the setting of breast malignancies, responses to immune checkpoint inhibitors (ICI) both low and ephemeral, particularly hormone receptor-positive (HR+) subtype. Effective predicated on abundant neoantigens, activity effector cells within tumor microenvironment (TME), establishment immunological memory. These prerequisites pose significant efficacy...
Generalized pustular psoriasis (GPP) constitutes a rare, severe inflammatory disorder that differs from vulgaris (PV). The IL-36 pathway has been identified as key element in GPP pathogenesis. To explore protein expression between PV and GPP, providing insights into potential mechanisms. We performed proteomic analysis of tissue specimens patients with to identify differentially expressed proteins. Comparative the data was proteins significant differences were further using...
Abstract Spatial transcriptomics and proteomics have independently transformed our understanding of complex biological processes; however, integration these modalities is currently limited. To overcome this challenge, we developed S patial P r O tein T ranscriptome equencing (SPOTS) for high-throughput transcriptome protein profiling within the spatial context. Applying SPOTS to spleen breast cancer samples revealed that spatially-resolved multi-omic provides a comprehensive perspective on...
Mutations of VHL (a negative regulator hypoxia-inducible factors) have position-dependent distinct cancer phenotypes. Only two known inherited homozygous mutations exist and they cause polycythemia: Chuvash R200W Croatian H191D. We report a second polycythemic H191D homozygote distantly related to the first propositus. Three generations both families were genotyped for analysis shared ancestry. Biochemical molecular tests performed better define their phenotypes, with an emphasis on...
Vertebrate myoblast fusion allows for multinucleated muscle fibers to compound the size and strength of mononucleated cells, but evolution this important process is unknown. We investigated evolutionary origins function membrane-coalescing agents Myomaker Myomixer in various groups chordates. Here, we report that likely arose through gene duplication last common ancestor tunicates vertebrates, while appears have evolved de novo early vertebrates. Functional tests revealed a complex history...
Tumor-infiltrating macrophages support critical steps in tumor progression, and their accumulation the microenvironment (TME) is associated with adverse outcomes therapeutic resistance across human cancers. In TME, adopt diverse phenotypic alterations, giving rise to heterogeneous immune activation states induction of cell cycle. While transcriptional profiles these are well-annotated cancers, underlying signals that regulate macrophage heterogeneity remain incompletely understood. Here, we...
Background Bone changes are common in sickle cell disease, but the pathogenesis is not fully understood. Tartrate-resistant acid phosphatase (TRACP) type 5b produced by bone-resorbing osteoclasts. In other forms of hemolytic anemia, increased iron stores associated with osteoporosis. We hypothesized that transfusional overload would be osteoclast activity patients disease.Design and Methods examined tartrate-resistant concentrations disease normal controls similar age sex distribution at...
Melanoma is known as the most aggressive and lethal type of cutaneous cancer due to its rapid development drug resistance chemotherapy drugs.In our study, we conducted a variety studies, including quantitative PCR, Western blot, autophagy apoptosis assays investigate involvement miR-26a HMGB1 in modulation dabrafenib sensitivity human melanoma cell lines.Our studies revealed that expressions were altered two lines after treatment. Additionally, caused this autophagic process was regulated by...
Abstract In vertebrates, multipotent progenitors located in the pharyngeal mesoderm form cardiomyocytes and branchiomeric head muscles, but dynamic gene expression programs mechanisms underlying cardiopharyngeal multipotency heart vs. muscle fate choices remain elusive. Here, we used single cell genomics simple chordate model Ciona, to reconstruct developmental trajectories forming first second lineages, precursors, characterize molecular underpinnings of choices. We show that FGF-MAPK...
In Chuvash polycythemia, homozygous von Hippel-Lindau (VHL) 598C>T leads to increased hypoxia inducible factor-1α and 2α, thromboses lower systemic blood pressures. Circulating homocysteine, glutathione, γ-glutamyltransferase cysteinylglycine concentrations were higher in 34 VHL598C>T homozygotes than 37 normal controls cysteine was lower. Multivariate analysis showed elevated homocysteine independently associated with mean pressures glutathione a similar degree. Among homozygotes, low...
Abstract The size of an animal is determined by the its musculoskeletal system. Myoblast fusion innovative mechanism that allows for multinucleated muscle fibers to compound and strength individual mononucleated cells. However, evolutionary history control underlying this important process currently unknown. phylum Chordata hosts closely related groups span distinct myoblast states: no in cephalochordates, restricted multinucleation tunicates, extensive, obligatory vertebrates. To elucidate...
Abstract Tumor-infiltrating macrophages support critical steps in tumor progression, and their accumulation the microenvironment (TME) is associated with adverse outcomes therapeutic resistance across human cancers. In TME, adopt diverse phenotypic alterations, giving rise to heterogeneous immune activation states induction of cell cycle. While transcriptional profiles these are well-annotated cancers, underlying signals that regulate macrophage heterogeneity remain incompletely understood....
Recent studies have shown that novel biologics may provide significant clinical benefits for patients with generalized pustular psoriasis (GPP). Ustekinumab and secukinumab been approved in Japan GPP treatment adult patients. However, the differences efficacy safety of these two drugs are not known.
Abstract CARM1 is a cancer-relevant protein arginine methyltransferase that regulates many aspects of transcription. Its pharmacological inhibition promising anti-cancer strategy. Here SKI-73 presented as chemical probe with pro-drug properties. can rapidly penetrate cell membranes and then be processed into active inhibitors, which are retained intracellularly 10-fold enrichment for days. These compounds were characterized their potency, selectivity, modes action, on-target engagement....
ABSTRACT The hemizygous R47H variant of TREM2 , a microglia-specific gene in the brain, increases risk for late-onset Alzheimer’s disease (AD). In this study, we identified subpopulation microglia with disease-enhancing proinflammatory signatures associated mutation human AD brains and tauopathy mouse brains. Using transcriptomic analysis at single-nuclei level from patients or common (CV )-TREM2 matched sex, pathology APOE status, found that was cell type- sex-specific transcriptional...