Lingling Huang
A5087894636- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Advanced Proteomics Techniques and Applications
- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- vaccines and immunoinformatics approaches
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Mass Spectrometry Techniques and Applications
- Fuel Cells and Related Materials
- Thyroid Cancer Diagnosis and Treatment
- Thyroid Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Electrocatalysts for Energy Conversion
- Catalytic Processes in Materials Science
Westlake University
2022-2023
Second Artillery General Hospital of Chinese People's Liberation Army
2023
Abstract Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in training cohort (n =...
Data-independent acquisition (DIA) mass spectrometry-based proteomics generates reproducible proteome data. The complex processing of the DIA data has led to development multiple analysis tools. In this study, we assessed performance five tools (OpenSWATH, EncyclopeDIA, Skyline, DIA-NN, and Spectronaut) using six datasets obtained from TripleTOF, Orbitrap, TimsTOF Pro instruments. By comparing identification quantification metrics examining shared unique cross-tool identifications, evaluated...
Abstract Although the development of COVID-19 vaccines has been a remarkable success, heterogeneous individual antibody generation and decline over time are unknown still hard to predict. In this study, blood samples were collected from 163 participants who next received two doses an inactivated vaccine (CoronaVac®) at 28-day interval. Using TMT-based proteomics, we identified 1,715 serum 7,342 peripheral mononuclear cells (PBMCs) proteins. We proposed sets potential biomarkers (seven serum,...
The diagnosis of follicular-patterned thyroid tumors such as follicular adenoma (FA), carcinoma (FTC), and variant papillary (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three by proteomic analysis. A pressure cycling technology (PCT)-data-independent acquisition (DIA) mass spectrometry workflow was employed investigate protein alterations in 52 formalin-fixed paraffin-embedded (FFPE) specimens: 18 FA, 15 FTC, 19 FvPTC specimens....
Prostate cancer (PCa) is the second most prevalent malignancy and fifth cause of cancer-related deaths in men. A crucial challenge identifying population at risk rapid progression from hormone-sensitive prostate (HSPC) to lethal castration-resistant (CRPC). We collected 78 HSPC biopsies measured their proteomes using pressure cycling technology a pulsed data-independent acquisition pipeline. quantified 7355 proteins these biopsies. total 251 showed differential expression between patients...
Abstract Although the development of COVID-19 vaccines has been a remarkable success, heterogeneous individual antibody generation and decline over time are unknown still hard to predict. In this study, blood samples were collected from 163 participants who next received two doses an inactivated vaccine (CoronaVac ® ) at 28-day interval. Using TMT-based proteomics, we identified 1715 serum 7342 peripheral mononuclear cells (PBMCs) proteins. We proposed sets potential biomarkers (seven serum,...
<div>Abstract<p>Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in...
<div>Abstract<p>Intrahepatic cholangiocarcinoma (ICC) is a relatively rare but highly aggressive tumor type that responds poorly to chemotherapy and immunotherapy. Comprehensive molecular characterization of ICC essential for the development novel therapeutics. Here, we constructed two independent cohorts from clinic centers. A comprehensive multiomics analysis via proteomic, whole-exome sequencing (WES), single-cell RNA (scRNA-seq) was performed. Novel subtypes were derived in...
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
Supplementary Figure from Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing–Assisted Multiomics Analysis
A Metal-organic-frameworks (MOFs) derived 3D porous nitrogen-rich octahedral carbon (PNOC) catalyst is successfully fabricated via carbonization of MIL-101(Fe) precursor process. As-obtained PNOC combines the advantages high surface area, well permeable pore and rich nitrogen content in one catalyst, which shows excellent catalytic activity stability for oxygen reduction reaction (ORR). This work would offer some new insights to advanced ORR electrocatalyst material designed fuel cells.
Abstract Background Prostate cancer (PCa) is the second most prevalent malignancy and fifth cause of cancer-related deaths in men. A crucial challenge identifying population at risk rapid progression from hormone-sensitive PCa (HSPC) to lethal castration-resistant (CRPC). Methods We collected 78 HSPC biopsies measured their proteomes using pressure cycling technology a pulsed data-independent acquisition pipeline. The proteomics data clinical metadata were used generate models for...