A5073787622- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Hair Growth and Disorders
- Prenatal Substance Exposure Effects
- Developmental Biology and Gene Regulation
- Aldose Reductase and Taurine
- dental development and anomalies
- Skin and Cellular Biology Research
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Tuberous Sclerosis Complex Research
- Digestive system and related health
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- Peptidase Inhibition and Analysis
- Reproductive System and Pregnancy
- Endoplasmic Reticulum Stress and Disease
- Connective tissue disorders research
- Plant tissue culture and regeneration
- Ovarian function and disorders
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Pulmonary Hypertension Research and Treatments
- Cancer Cells and Metastasis
Shandong University
2017-2021
Zhuhai People's Hospital
2019
Creative Commons
2017
Lishui City People's Hospital
2016
University of Rochester Medical Center
2009-2015
Affiliated Hospital of North Sichuan Medical College
2014
University of South China
2013
China Agricultural University
2010-2012
CAE (Canada)
2011
National University of Singapore
2007
Members of the Wnt family are secreted glycoproteins that trigger cellular signals essential for proper development organisms. Cellular signaling induced by proteins is involved in diverse developmental processes and human diseases. Previous studies have generated an enormous wealth knowledge on events signal-receiving cells. However, relatively little known about making signal-producing Here, we describe Gpr177 , mouse orthologue Drosophila Wls expressed during formation embryonic axes....
Abstract We have previously demonstrated that Gpr177 , the mouse orthologue of Drosophila Wls / Evi Srt is required for establishment anterior–posterior axis. The null phenotype highly reminiscent to loss Wnt3, earliest abnormality among all Wnt knockouts in mice. expression various cell types and tissues lead us hypothesize reciprocal regulation essential Wnt‐dependent developmental pathogenic processes. Here, we create a new strain permitting conditional inactivation . Wnt1‐expressing...
Epidermal stratification of the mammalian skin requires proliferative basal progenitors to generate intermediate cells that separate from layer and are replaced by post-mitotic cells. Although Wnt signaling has been implicated in this developmental process, mechanism underlying Wnt-mediated regulation remains elusive. Here we show secreted is not required for their differentiation. However, epidermal production Wnts essential formation spinous through modulation a BMP-FGF cascade dermis. The...
Post-translational modification of proteins by small ubiquitin-related modifier (SUMO) is reversible and highly evolutionarily conserved from yeasts to humans. Unlike ubiquitination with a well-established role in protein degradation, sumoylation may alter function, activity, stability subcellular localization. Members SUMO-specific protease (SENP) family, capable SUMO removal, are involved the reversed conjugation process. Although proteases known reverse many well-defined systems, their...
Mutations in the APC or β-catenin genes are well-established initiators of colorectal cancer, yet modifiers that facilitate survival and progression nascent tumor cells not well defined. Using genetic pharmacologic approaches mouse cancer human xenograft models, we show incipient intestinal activate CDC42, an APC-interacting small GTPase, as a crucial step malignant progression. In mouse, Cdc42 ablation attenuated tumorigenicity mutant carrying single mutations. Similarly, with relatively...
Communication between stem and niche supporting cells maintains the homeostasis of adult tissues. Wnt signaling is a crucial regulator cell niche, but mechanism that governs ligand delivery in this compartment has not been fully investigated. We identified secretion partly dependent on Rab8a-mediated anterograde transport Gpr177 (wntless), Wnt-specific transmembrane transporter. binds to Rab8a, depletion which compromises traffic, thereby weakening multiple Wnts. Analyses generic...
Abstract Background Mouse genetic study has demonstrated that Axin2 is essential for calvarial development and disease. Haploid deficiency of β-catenin alleviates the phenotype caused by deficiency. This loss-of-function provides evidence requirement in exerting downstream effects Axin2. Results Here we utilize a gain-of-function analysis to further assess role β-catenin. A transgenic expression system permitting conditional activation spatiotemporal specific manner been developed. Aberrant...
Establishment of the functional pulmonary vasculature requires intimate interaction between epithelium and mesenchyme. Previous genetic studies have led to inconsistent conclusions about contribution epithelial Wnts development. This discrepancy is possibly due redundancy among different Wnts. Here, we use Shh-Cre conditionally delete Gpr177 (the mouse ortholog Drosophila Wntless, Wls), a chaperon protein important for sorting secretion Wnt proteins. Deletion reduces signaling activity in...
Abstract Wls/Evi/Srt encoding a multipass transmembrane protein has been identified as regulator for proper sorting and secretion of Wnt in flies. We have previously demonstrated that Gpr177 is the mouse ortholog required axis determination. transcriptional target activated to assist its subcellular distribution feedback regulatory loop. We, therefore, proposed reciprocal regulation essential Wnt‐dependent developmental pathogenic processes. Here, we examine expression pattern development....
Aberrant regulation of the Wnt pathway, essential for various developmental processes, is tightly linked to human breast cancers. By hijacking this evolutionary conserved signaling cancer cells acquire sustaining proliferation ability, leading modification physiologic properties necessary tumor initiation and progression. An enormous wealth knowledge on importance in development has been obtained, but cell types responsible production proliferative signal operating within normal malignant...
Abstract Background The DENND1A has been identified as a guanine nucleotide exchange factor for small GTPase Rab35, which functions in endocytic trafficking to mediate the recycling of selective cargos. Genetic alterations within gene have implicated human disease such polycystic ovary syndrome (PCOS). However, role developmental and reproductive processes is largely unknown. Results Using Dennd1a knockout mice, we uncovered that homogeneous Dennd1a−/− mutants died around embryonic day (E)...
Abstract Oxidative stress caused by hyperglycemia is one of the key factors responsible for maternal diabetes‐induced congenital malformations, including neural tube defects in embryos. However, mechanisms which diabetes induces oxidative during neurulation are not clear. The present study was aimed to investigate whether high glucose stem cells (NSCs), compose development. We also investigated mechanism disturbs growth and survival NSCs vitro . were exposed physiological d ‐glucose...
Glioblastoma (GBM) is the most aggressive and highly invasive type of astrocytic tumors. Despite advances in diagnosis therapy, prognosis survival time remain poor. Identifying key mediators tumor cell proliferation, migration, invasion crucial to development new more effective therapies. In this paper, we report novel role Spermatogenesis‐ oogenesis‐ specific basic helix‐loop‐helix transcription factor1 (Sohlh1) inhibition Wnt/β‐catenin signaling behaviors GBM cells. Immunohistochemistry...
The purpose was to explore the clinical effects and safety of gemcitabine plus nedaplatin in treatment advanced nasopharyngeal carcinoma.From March 2014 August 2015, we recruited 63 carcinoma patients our hospital. Moreover, 62 cases were randomly divided into control group (n = 31) 32). Patients groups treated with 5-fluorouracil 500 mg/m 2 + ml 0.9% sodium chloride injection intervenous drop infusion day 1-5 cisplatin 20 21 days per cycle for 3 cycles; given 1000 1 8 cycles. objective...
Communication between stem and niche supporting cells maintains the homeostasis of adult tissues. Wnt signaling is a crucial regulator cell niche, but mechanism that governs ligand delivery in this compartment has not been fully investigated. We identified secretion partly dependent on Rab8a-mediated anterograde transport Gpr177 (wntless), Wnt-specific transmembrane transporter. binds to Rab8a, depletion which compromises traffic, thereby weakening multiple Wnts. Analyses generic...
The molecular mechanisms of hair initiation and growth is one the long-standing research programs in developmental biology. It has been suggested that for decades existence an inducing factor dermis required development. Furthermore, various signaling families are involved reciprocal interactions between epidermis during follicle morphogenesis. Among them, wingless-type MMTV integration site family (Wnt) probably earliest most critical regulator. This review primarily focuses on role Wnt...
Background: The DENN (differentially expressed in neoplastic versus normal cells) domain containing 1A (Dennd1a), a guanine nucleotide exchange factor (GEF) for the small GTPase Rab35, is essential mouse embryogenesis. Disruption of Dennd1a impairs migration and differentiation fetal germ cells. In present study, we further elucidated role oogenesis meiosis ovary. Results: Ablation disrupted mRNA expression Sohlh2, Figla, Stra8, Rec8 ovary Dennd1a-/- mutants at E13.5. Using ex vivo culture...