A5004785994- Neonatal Respiratory Health Research
- Carcinogens and Genotoxicity Assessment
- Arsenic contamination and mitigation
- Tissue Engineering and Regenerative Medicine
- Tracheal and airway disorders
- Congenital Diaphragmatic Hernia Studies
- Immune Cell Function and Interaction
- interferon and immune responses
- Immune Response and Inflammation
- Occupational and environmental lung diseases
- Renal and related cancers
- Epigenetics and DNA Methylation
- Electrospun Nanofibers in Biomedical Applications
- Mitochondrial Function and Pathology
- T-cell and B-cell Immunology
- Data-Driven Disease Surveillance
- Energy Harvesting in Wireless Networks
- Esophageal and GI Pathology
- Misinformation and Its Impacts
- Cytokine Signaling Pathways and Interactions
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
Discovery Institute
2020-2022
Sanford Burnham Prebys Medical Discovery Institute
2020-2022
The University of Texas Health Science Center at Houston
2019-2022
Brown Foundation
2018-2022
UNSW Sydney
2022
Boston University
2022
Columbia University
2006-2021
Barnard College
2021
University of Pennsylvania
2019-2020
Columbia University Irving Medical Center
2008-2019
Severe influenza disease strikes otherwise healthy children and remains unexplained. We report compound heterozygous null mutations in IRF7, which encodes the transcription factor interferon regulatory 7, an child who suffered life-threatening during primary infection. In response to virus, patient's leukocytes plasmacytoid dendritic cells produced very little type I III interferons (IFNs). Moreover, dermal fibroblasts induced pluripotent stem cell (iPSC)-derived pulmonary epithelial reduced...
Autosomal recessive IRF7 and IRF9 deficiencies impair type I III IFN immunity underlie severe influenza pneumonitis. We report three unrelated children with A virus (IAV) infection manifesting as acute respiratory distress syndrome (IAV-ARDS), heterozygous for rare TLR3 variants (P554S in two patients P680L the third) causing autosomal dominant (AD) deficiency. AD deficiency can herpes simplex virus-1 (HSV-1) encephalitis (HSE) by impairing cortical neuron-intrinsic to HSV-1. TLR3-mutated...
We propose a new approach to lung regeneration by replacement of damaged epithelium with full preservation vasculature.
Cancer models based on cells derived from human embryonic stem (hESCs) may reveal why certain constellations of genetic changes drive carcinogenesis in specialized lineages. Here we demonstrate that inhibition NOTCH signaling induces up to 10% lung progenitor form pulmonary neuroendocrine (PNECs), putative precursors small cell cancers (SCLCs), and can increase PNECs by reducing levels retinoblastoma (RB) proteins with inhibitory RNA. Reducing TP53 protein or expressing mutant KRAS EGFR...
Abstract Arsenic is a well-established human carcinogen that chronically consumed in drinking water by millions of people worldwide. Recent evidence has suggested arsenic genotoxic carcinogen. Furthermore, we have shown mitochondria mediate the mutagenic effects mammalian cells, as did not induce nuclear mutations mitochondrial DNA (mtDNA)–depleted cells. Using human-hamster hybrid AL show here alters function decreasing cytochrome c oxidase and oxygen consumption but increasing citrate...
The entire lung epithelium arises from SRY box 9 (SOX9)-expressing progenitors that form the respiratory tree and differentiate into airway alveolar cells. Despite progress in understanding their initial specification within embryonic foregut, how these are subsequently maintained is less clear. Using inducible, progenitor-specific genetic mosaic mouse models, we showed β-catenin (CTNNB1) maintains by promoting a hierarchical progenitor gene signature, suppressing gastrointestinal (GI)...
The RB1 gene is frequently mutated in human cancers but its role tumorigenesis remains incompletely defined. Using an induced pluripotent stem cell (iPSC) model of hereditary retinoblastoma (RB), we report that the spliceosome up-regulated target responding to oncogenic stress RB1-mutant cells. By investigating transcriptomes and genome occupancies RB iPSC–derived osteoblasts (OBs), discover both E2F3a, which mediates spliceosomal expression, pRB, antagonizes coregulate more than one-third...
Human small airway epithelial cells (SAECs) previously immortalized with human telomerase reverse transcriptase (h-TERT) were continuously treated sodium arsenite at a dose of 0.5 µg/mL in culture for up to 6 months. Arsenic-treated progressively displayed an increase transformed phenotype including enhanced growth saturation density, plating efficiency, and anchorage-independent invasion capability compared their nontreated control cells. To determine whether arsenic-induced cell...
The incidence of asbestos-induced human cancers is increasing worldwide, and considerable evidence suggests that reactive oxygen species (ROS) are important mediators these diseases. Our previous studies suggested mitochondria might be involved in the initiation oxidative stress asbestos-exposed mammalian cells.We investigated whether a potential cytoplasmic target asbestos using mitochondrial DNA-depleted (ρ(0)) small airway epithelial (SAE) cell model: ρ(0) SAE cells lack capacity to...
Background Substance use by youth remains a significant public health concern. Social media provides the opportunity to discuss and display substance use–related beliefs behaviors, suggesting that act of posting drug-related content, or viewing posted may influence in youth. This aligns with empirically supported theories, which posit behavior is influenced perceptions normative behavior. Nevertheless, few studies have explored content posts related use. Objective study aimed identify...
People are increasingly getting information and news from social media. On Twitter we seeing the emergence of "tweetorials" -- long, explanatory threads written by experts. In this work study tweetorials as a form science writing. While scientists have begun to champion importance communication medium, few studied how people successfully using medium communicate complex nuanced ideas. To understand work, curated collection 46 clear engaging multiple domains. We analyzed these for writing...
The Btla inhibitory receptor limits innate and adaptive immune responses, both preventing the development of autoimmune disease restraining anti-viral anti-tumor responses. It remains unclear how functions in diverse lymphocytes contribute to immunoregulation. Here, we show that inhibits activation genes regulating metabolism cytokine signaling, including Il6 Hif1a, indicating a regulatory role humoral immunity. Within mucosal Peyer's patches, find T-cell-expressed Btla-regulated Tfh cells,...
Inorganic arsenic is a well-known human skin carcinogen. Chronic exposure results in various types of lesions, including squamous cell carcinoma (SCC). To investigate whether mutant stem cells participate arsenic-associated carcinogenesis, we repeatedly exposed the HaCaT line to an environmentally relevant level (0.05 ppm) vitro for 18 weeks. Following sodium arsenite administration, cycle, colony-forming efficiency (CFE), tumorigenicity, and expression CD44v6, NF-κB p53, were analyzed at...
Background Adolescents and young adults in the age range of 13-24 years are at highest risk developing HIV infections. As social media platforms extremely popular among youths, researchers can utilize these to curb epidemic by investigating associations between discourses on infections epidemiological data Objective The goal this study was examine how Twitter activity men is related incidence infection population. Methods We used integrated human-computer techniques characterize HIV-related...