A5053758451- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Nuclear Receptors and Signaling
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Neonatal Respiratory Health Research
- RNA Interference and Gene Delivery
- Polyomavirus and related diseases
- Pregnancy and preeclampsia studies
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Histiocytic Disorders and Treatments
- Cancer Cells and Metastasis
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Renal and related cancers
- Platelet Disorders and Treatments
- Histone Deacetylase Inhibitors Research
- Mesenchymal stem cell research
- Systemic Sclerosis and Related Diseases
- Clusterin in disease pathology
- Autophagy in Disease and Therapy
- Kruppel-like factors research
University of Pennsylvania
2019-2025
Cancer Research Institute
2019-2022
Cancer Research Institute of the Slovak Academy of Sciences
2020-2022
California University of Pennsylvania
2022
EIA University
2016-2018
Columbia University Irving Medical Center
2013-2017
Cancer Research Center
2017
Icahn School of Medicine at Mount Sinai
2012
National Institutes of Health
2010
National Cancer Institute
2010
Cancer stem cells are presumed to have virtually unlimited proliferative and self-renewal abilities be highly resistant chemotherapy, a feature that is associated with overexpression of ATP-binding cassette transporters. We investigated whether prolonged continuous selection for drug resistance enriches cultures cancer stem-like cells.Cancer were defined as CD44+/CD24⁻ could self-renew (ie, generate the tumorigenic phenotype), differentiate, invade, form tumors in vivo. used...
Idiopathic multicentric Castleman disease (iMCD) is a rare and poorly-understood cytokine storm-driven inflammatory disorder. Interleukin-6 (IL-6) known driver in some patients, but anti-IL-6 therapy with siltuximab not effective all biomarkers indicating success at an early time point following treatment initiation are lacking. Here we show, by comparison of levels 1,178 proteins sera healthy participants (N = 42), patients iMCD 88), related diseases 60), comprehensive landscape candidate...
Summary Idiopathic multicentric Castleman disease (iMCD) is a rare haematological disorder characterized by generalized lymphadenopathy with atypical histopathological features and systemic inflammation caused cytokine storm involving interleukin‐6 (IL‐6). Three clinical subtypes are recognized: thrombocytopenia, anasarca, fever, renal dysfunction, organomegaly (iMCD‐TAFRO); idiopathic plasmacytic (iMCD‐IPL), thrombocytosis hypergammaglobulinaemia; iMCD‐not otherwise specified (iMCD‐NOS),...
Abstract Castleman disease (CD) is a rare hematologic disorder characterized by pathologic lymph node changes and range of symptoms due to excessive cytokine production. While uncontrolled infection with human herpesvirus-8 (HHV-8) responsible for the storm in portion multicentric CD (HHV-8-associated MCD) cases, etiology unicentric (UCD) HHV-8-negative/idiopathic MCD (iMCD) unknown. Several hypotheses have been proposed regarding pathogenesis UCD iMCD, including occult given precedent...
Introduction Idiopathic Multicentric Castleman Disease (iMCD) is a polyclonal lymphoproliferative disorder involving cytokine storms that can lead to organ failure and death. The cause of iMCD unknown, but some clinical evidence suggests an autoimmune etiology. For example, connective tissue disorders (CTDs) share many features, autoantibodies have been anecdotally reported in individual patients. This study investigates whether common are shared across Methods We assembled custom bead-based...
Abstract The developmental fate of hematopoietic stem and progenitor cells is influenced by their physiological context. Although most are found in the bone marrow adult, some other tissues, including spleen. extent to which determined tissue they reside not clear. In this study, we identify a new population, enriched mouse spleen, defined cKit+CD71lowCD24high expression. This previously uncharacterized population generates exclusively myeloid lineage cells, erythrocytes, platelets,...
Summary Hematopoietic stem and progenitor cells (HSPCs) differentiate from hemogenic endothelial (HE) through an to hematopoietic cell transition (EHT). Newly formed HSPCs accumulate in intra-arterial clusters (IACs) before colonizing the fetal liver. To examine molecular transitions during EHT, heterogeneity of within IACs, we profiled ∼37,000 caudal arteries embryonic day 9.5 (E9.5) E11.5 mouse embryos by single-cell transcriptome chromatin accessibility sequencing. We identified...
ABSTRACT Hemogenic endothelial (HE) cells in the dorsal aorta undergo an endothelial-to-hematopoietic transition (EHT) to form multipotent progenitors, lympho-myeloid biased progenitors (LMPs), pre-hematopoietic stem (pre-HSCs) and adult-repopulating HSCs. These briefly accumulate intra-arterial hematopoietic clusters (IAHCs) before being released into circulation. It is generally assumed that number of IAHC correlates with Here, we show changes cells, LMPs HSCs can be uncoupled. Mutations...
Abstract Hemogenic endothelial (HE) cells in the dorsal aorta undergo an to hematopoietic transition (EHT) form lympho-myeloid biased progenitors (LMPs), pre-hematopoietic stem (pre-HSCs) and adult-repopulating HSCs. These briefly accumulate intra-arterial clusters (IAHCs) before being released into circulation. It is generally assumed that number of IAHC correlates with Here we show changes cells, LMPs, HSCs can be uncoupled. Mutations impairing MyD88-dependent toll-like receptor (TLR)...
Abstract Adult hematopoiesis occurs primarily in the bone marrow (BM), generating mature blood cells as well maintaining a heterogeneous pool of self-renewing hematopoietic stem (HSCs). However, studies suggest that BM may not be only site adult hematopoiesis: during times distress stemming from radiation or disease, spleen can independently reconstitute blood. Given does indeed contain small, resident population lineage-sca1+ckit+CD48-CD150+ long-term (LT)-HSCs with potent reconstitution...
Abstract Studies of hematopoietic stem cells (HSCs) have advanced our understanding the intracellular signals and microenvironmental interactions that influence HSC fate. Our lab is interested in role orphan nuclear receptor NR4A1, an immediate response gene sensitive to external stimuli, development. NR4A1 regulates development specific, mature cell lineages from both innate adaptive immune system including patrolling monocytes, a myeloid subset. More recently, we shown expression also...