A5100374797- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Acute Ischemic Stroke Management
- Genomics and Chromatin Dynamics
- Neurological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- COVID-19 Impact on Reproduction
- Neuroscience and Neural Engineering
- Alzheimer's disease research and treatments
- Reproductive Biology and Fertility
- Neurological Disease Mechanisms and Treatments
- Amyotrophic Lateral Sclerosis Research
- Stroke Rehabilitation and Recovery
- Animal Genetics and Reproduction
- Transcranial Magnetic Stimulation Studies
- Virus-based gene therapy research
- Ion Channels and Receptors
- Vagus Nerve Stimulation Research
- Cancer Genomics and Diagnostics
Qingdao Agricultural University
2025
Westlake University
2023-2024
Australian Regenerative Medicine Institute
2014-2023
Monash University
2014-2023
Shanghai University of Sport
2022-2023
Chinese University of Hong Kong
2021
University of Kentucky
2019
Discovery Institute
2018-2019
Taihe Hospital
2019
Hubei University of Medicine
2019
Abstract The role of microglia cells in Alzheimer’s disease (AD) is well recognized, however their molecular and functional diversity remain unclear. Here, we isolated amyloid plaque-containing (using labelling with methoxy-XO4, XO4 + ) non-containing (XO4 − from an AD mouse model. Transcriptomics analysis identified different transcriptional trajectories ageing mice. microglial transcriptomes demonstrated dysregulated expression genes associated late onset AD. We further showed that the...
Cells undergo a major epigenome reconfiguration when reprogrammed to human induced pluripotent stem cells (hiPS cells). However, the epigenomes of hiPS and embryonic (hES) differ significantly, which affects cell function1-8. These differences include epigenetic memory aberrations that emerge during reprogramming, for mechanisms remain unknown. Here we characterized persistence emergence these by performing genome-wide DNA methylation profiling throughout primed naive reprogramming somatic...
Abstract Recent developments of sequencing-based spatial transcriptomics (sST) have catalyzed important advancements by facilitating transcriptome-scale gene expression measurement. Despite this progress, efforts to comprehensively benchmark different platforms are currently lacking. The extant variability across technologies and datasets poses challenges in formulating standardized evaluation metrics. In study, we established a collection reference tissues regions characterized well-defined...
Hexanucleotide repeat expansions in the C9orf72 gene are most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. Due to lack trunk neuromuscular organoids (NMOs) from ALS patients' induced pluripotent stem cells (iPSCs), an organoid system was missing model spinal neurodegeneration. With patient-derived iPSCs isogenic controls, we used NMO containing cord neural peripheral muscular tissues show that NMOs could defects ALS, including contraction weakness,...
Abstract SARS-CoV-2 infection causes COVID-19. Several clinical reports have linked COVID-19 during pregnancy to negative birth outcomes and placentitis. However, the pathophysiological mechanisms underpinning placentation early are not clear. Here, shed light on this, we used induced trophoblast stem cells generate an in vitro placenta model. We identified that syncytiotrophoblasts could be infected through angiotensin-converting enzyme 2 (ACE2). Using a co-culture model of vertical...
Quorum sensing (QS) is a bacterial intercellular communication system that can regulate the expression of various virulence genes coordinate group behaviors bacteria by concentration signaling molecules in surrounding environment. An increase drug-resistance has been caused widespread use antibiotics, making it urgent to identify safe and effective alternatives antibiotics. quenching (QQ) strategy control infections disrupting QS system, which reduces pathogenicity or increases biofilm...
Azithromycin (AZM) and other macrolide antibiotics are applied as immunomodulatory treatments for CNS disorders. The antibiotic properties of AZM purportedly independent.To improve the efficacy reduce resistance risk AZM-based therapies, we evaluated neuroprotective novel derivatives. We semisynthetically prepared derivatives by altering sugar moieties established important inhibiting bacterial protein synthesis. Bone marrow-derived macrophages (BMDMs) were stimulated in vitro with...
Abstract The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation seven new mAbs that detect cell surface proteins present on live fixed ES (hESCs) iPS (hiPSCs), confirming our previous prediction these were hPSCs. all show a high correlation with POU5F1 (OCT4) expression other hPSC markers (TRA-160 SSEA-4) in cultures rare OCT4 positive...
The need to derive and culture diverse cell or tissue types in vitro has prompted investigations on how changes conditions affect states. However, the identification of optimal (e.g., signaling molecules growth factors) required maintain convert between remains a time-consuming task. Here, we developed EpiMogrify, an approach that leverages data from ∼100 human cell/tissue available ENCODE Roadmap Epigenomics consortia predict factors can either identity enhance directed differentiation (or...
The impact of aging on intestinal stem cells (ISCs) has not been fully elucidated. In this study, we identified widespread epigenetic and transcriptional alterations in old ISCs. Using a reprogramming algorithm, set key transcription factors (Egr1, Irf1, FosB) that drives molecular functional differences between young states. Overall, by dissecting the signature aged ISCs, our study enhance regenerative capacity
The canonical Wnt/β-catenin pathway is crucial for early embryonic patterning, tissue homeostasis, and regeneration. While stimulation has been used extensively to modulate pluripotency differentiation of pluripotent stem cells (PSCs), the mechanism these two seemingly opposing roles not fully characterized currently largely attributed activation nuclear Wnt target genes. Here, we show that low levels via ectopic expression Wnt1 or administration glycogen synthase kinase-3 inhibitor...
Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria his unaffected sibling. Induced pluripotent stem developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, c-MYC. All four iPSC lines displayed cell morphology, pluripotency-associated at DNA protein level, alkaline phosphatase enzymatic activity...
Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in epigenome profiling techniques have significantly increased our understanding cell-specific utilization REs. However, it remains difficult dissect majority that interact these REs due lack appropriate techniques. Therefore, we developed TINC: TALE-mediated isolation nuclear chromatin. Using this new method, interrogated protein complex...