A5051277880- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Neonatal Respiratory Health Research
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Pulmonary Hypertension Research and Treatments
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Transplantation: Methods and Outcomes
- Muscle Physiology and Disorders
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Viral Infections and Vectors
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Circadian rhythm and melatonin
- Cancer-related molecular mechanisms research
- Liver physiology and pathology
- Biomedical Ethics and Regulation
- Single-cell and spatial transcriptomics
Rhode Island Hospital
2015-2025
Brown University
2015-2025
Providence College
2013-2024
Cancer Institute (WIA)
2024
Lifespan
2020-2024
University of Warwick
2017-2020
Miriam Hospital
2013-2014
Bank of Canada
2013
Roger Williams Medical Center
2001-2007
University of Massachusetts Chan Medical School
1997-2004
Extracellular vesicles (EVs) from mice with monocrotaline (MCT)-induced pulmonary hypertension (PH) induce PH in healthy mice, and the exosomes (EXO) fraction of EVs mesenchymal stem cells (MSCs) can blunt development hypoxic PH. We sought to determine whether EXO is responsible for modulating vascular responses differences EXO-miR content explains differential effects EXOs MSCs MCT-PH.Plasma, lung MCT-PH, control were divided into (exosome), microvesicle (MV) fractions injected mice. EXO,...
Numerous animal studies have demonstrated that adult marrow-derived cells can contribute to the cellular component of lung. Lung injury is a major variable in this process; however, mechanism remains unknown. We hypothesize injured lung capable inducing epigenetic modifications marrow cells, influencing them assume phenotypic characteristics cells. report under certain conditions, radiation-injured induced expression pulmonary epithelial cell-specific genes and prosurfactant B protein...
The mechanism of hemopoietic stem cell homing to the bone marrow involves molecular interactions that mediate recognition and interaction these cells with microenvironment, including extracellular matrix. On selective binding, this environment, in combination soluble cytokines, regulates proliferation differentiation. Using immunofluorescence labeling, we analyzed location prominent matrix proteins fibronectin, collagen Types I, III, IV, laminin sections murine femoral marrow. Collagen...
Allogeneic and autologous marrow transplants are routinely used to correct a wide variety of diseases. In addition, potentially provide opportune means delivering genes in transfected, engrafting stem cells. However, relatively little is known about the mechanisms engraftment transplant recipients, especially nonablated setting with regard cells not hemopoietic origin. particular, this includes stromal progenitors osteoblastic lineage. We have demonstrated for first time that whole bone...
Early work on platelet and erythrocyte vesicles interpreted the phenomena as a discard of material from cells. Subsequently, were studied possible vaccines and, most recently, there has been focus effects cell fate. Recent studies have indicated that extracellular vesicles, previously referred to microvesicles or exosomes, capacity change phenotype neighboring Extensive shown derived either lung liver can enter bone marrow cells (this is prerequisite) alter their fate toward originating...
Mesenchymal stem cell extracellular vesicles attenuate pulmonary hypertension, but their ability to reverse established disease in larger animal models and the duration mechanism(s) of effect are unknown. We sought determine efficacy mechanism mesenchymal cells’ attenuating hypertension rats with Sugen/hypoxia-induced hypertension. Male were treated or an equal volume saline vehicle by tail vein injection before after subcutaneous Sugen 5416 exposure 3 weeks hypoxia. Pulmonary was assessed...
Abstract Aims Pulmonary arterial hypertension (PAH) is a fatal disease without cure. Previously, we found that transcription factor RUNX1-dependent haematopoietic transformation of endothelial progenitor cells may contribute to the pathogenesis PAH. However, therapeutic potential RUNX1 inhibition reverse established PAH remains unknown. In current study, aimed determine whether was sufficient Sugen/hypoxia (SuHx)-induced pulmonary (PH) in rats. We also demonstrate possible mechanisms...
The purpose of this study was to determine the prevalence p53 overexpression in feline oral squamous cell carcinomas (SCC) and determine, if any, association between lifestyle factors environmental exposures, including exposure tobacco smoke (ETS). Questionnaires concerning ETS other were sent owners cats presenting Harrington Oncology Program with a diagnosis SCC 1991 2000. Additionally, 23 formalin-fixed biopsy samples from these cats, information regarding ETS, evaluated...
Phage display peptide libraries have enabled the discovery of peptides that selectively target specific organs. Selection organ‐specific is mediated through binding displayed on phage coat protein to adhesion molecules expressed within targeted Hematopoietic stem cells home bone marrow, and certain receptors critical this function been demonstrated. Using a library, we identified trafficked murine marrow in vivo. We independently isolated exactly same heptapeptide from entire library by...
We have previously shown that injury induced by irradiation to murine marrow can be partially or completely reversed exposure human mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). Investigation of the biodistribution EVs in vivo is essential for understanding EV biology. In this study, we evaluated DiD lipid dye labeled MSC-EV mice under different conditions, including doses and injection schedules, time post injection, radiation. DiD-labeled MSC-EVs appeared highest liver...
Abstract Traumatic brain injury (TBI) is a common cause of death and acquired disability in adults children. Identifying biomarkers for mild TBI (mTBI) that can predict functional impairments on neuropsychiatric neurocognitive testing after head trauma yet to be firmly established. Extracellular vesicles (EVs) are known traffic from the oral cavity detected saliva. We hypothesize genetic profile salivary EVs patients who have suffered will differ normal healthy controls, thus constituting...
Exposure to high-dose radiation often results in hematopoietic acute syndromes, leading early mortality, while current therapies for patients exposed lethal doses are limited. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have shown promise tissue repair and regeneration but not been well investigated mitigating damage. We previously demonstrated that human or murine MSC-EVs can reverse bone marrow injury caused by mild moderate radiation. The study evaluated the therapeutic...
We studied the genetic and engraftment phenotype of highly purified murine hematopoietic stem cells (lineage negative, rhodamine-low, Hoechst-low) through cytokine-stimulated cell cycle. Cells were cultured in interleukin (IL)-3, IL-6, IL-11, steel factor for 0 to 48 h tested capacity a lethally irradiated competitive transplant model. Engraftment showed major fluctuations with nadirs at 36 culture recovery during next G1. Gene expression quiescent (0 h) or cycling (48 was compared lineage...
No AccessJournal of UrologyInvestigative Urology1 Nov 2010Microvesicle Induction Prostate Specific Gene Expression in Normal Human Bone Marrow Cells Joseph F. Renzulli, Michael Del Tatto, Gerri Dooner, Jason Aliotta, Lisa Goldstein, Mark Gerald Colvin, Devasis Chatterjee, and Peter Quesenberry RenzulliJoseph Renzulli Department Urology, Miriam Hospital, Providence, Rhode Island Warren Alpert School Medicine at Brown University, , TattoMichael Tatto Division Hematology/Oncology, Medicine,...
Background Interest has been generated in the capacity of cellular‐derived microvesicles to alter fate different target cells. Lung, liver, heart and brain‐derived vesicles can genetic phenotype murine marrow cells; however, stability such changes mechanism these remain unclear. In present work, we show that lung‐derived (LDMV) transcriptome proteome cells initially by mRNA regulator(s) transcription transfer, but long term change is due solely transfer a transcriptional regulator with cell....
Replication competent HIV-1 persists in a subpopulation of CD4+ T lymphocytes despite prolonged antiretroviral treatment. This residual reservoir infected cells harbors transcriptionally silent provirus capable reigniting productive infection upon discontinuation therapy. Certain classes drugs can activate latent virus but not at levels that lead to reductions size vivo. Here, we show the utility receptor targeting exosomes as an latency reversal agent (LRA). We engineered human cellular...
Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that has been implicated in numerous oncogenic processes. GSK-3 inhibitor elraglusib (9-ING-41) shown promising preclinical and clinical antitumor activity across multiple tumor types. Despite early-phase trial results, there have limited efforts to characterize the potential immunomodulatory properties of elraglusib. We report promotes immune cell-mediated cell killing microsatellite stable colorectal cancer (CRC) cells....