A5102909682- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Platelet Disorders and Treatments
- Neonatal Respiratory Health Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Blood groups and transfusion
- Tissue Engineering and Regenerative Medicine
- T-cell and Retrovirus Studies
- Blood transfusion and management
- T-cell and B-cell Immunology
- Fibroblast Growth Factor Research
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- Sexual function and dysfunction studies
- Inflammatory Biomarkers in Disease Prognosis
- Blood donation and transfusion practices
Inserm
2013-2025
Établissement Français du Sang
2016-2025
Université de Strasbourg
2013-2023
Laboratoire de Neurosciences Cognitives et Adaptatives
2023
Brown Foundation
2007-2017
The University of Texas Health Science Center at Houston
2009-2012
Peter MacCallum Cancer Centre
2001-2010
Institute for Molecular Medicine
2007
Institut de Radioprotection et de Sûreté Nucléaire
1998-2000
Institut Jacques Monod
1997
The cellular and molecular microenvironment of epithelial stem progenitor cells is poorly characterized despite well-documented roles in homeostatic tissue renewal, wound healing, cancer progression. Here, we demonstrate that, organotypic cocultures, dermal pericytes substantially enhanced the intrinsically low tissue-regenerative capacity human epidermal that have committed to differentiate this enhancement was independent angiogenesis. We used microarray analysis identify genes expressed...
Originally identified as a marker specifying murine hematopoietic stem cells, the Sca-1 antigen has since been shown to be differentially expressed by candidate cells in tissues including vascular endothelium, skeletal muscle, mammary gland, and prostate of adult mice. In lung, previously selectable for isolation nonhematopoietic (CD45(-)), nonendothelial (CD31(-)) bronchioalveolar (BASC) located at duct junction that coexpress surfactant protein C Clara cell specific protein. Our systematic...
Germ line or hypothalamus-specific deletion of Y2 receptors in mice results a doubling trabecular bone volume. However, the specific mechanism by which increases mass has not yet been identified. Here we show that cultured adherent marrow stromal cells from <i>Y2</i><sup>-/-</sup> also demonstrate increased mineralization <i>in vitro</i>. Isolation two populations progenitor cell types, an immature mesenchymal stem population and more highly differentiated cells, revealed greater number...
Microenvironment and activation signals likely imprint heterogeneity in the lymphatic endothelial cell (LEC) population. Particularly LECs of secondary lymphoid organs are exposed to different types immune stimuli. However, our understanding nature LEC their source within organ steady state remains incomplete. Here we show that integrin alpha 2b (ITGA2b), known be carried by platelets, megakaryocytes hematopoietic progenitors, is expressed a lymph node subset LECs, residing medullary,...
In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS(+)K) with long term reconstituting potential. contrast, poorly characterized LS(-)K fail to reconstitute lethally irradiated recipients. Relative quantification mass spectrometry transcriptional profiling were used characterize LS(+)K cells. This approach yielded data on >1200 proteins. Only 32% protein changes correlated mRNA modulation demonstrating post-translational regulation in early hematopoietic development. had...
Megakaryocytes (MKs) are the precursor cells of platelets, located in bone marrow (BM). Once mature, they extend elongated projections named proplatelets through sinusoid vessels, emerging from stroma into circulating blood. Not all signals microenvironment that regulate proplatelet formation understood, particularly those BM biomechanics. We sought to investigate how MKs perceive and adapt modifications stiffness their environment. Although is one softest tissue body, its rigidification...
Abstract The use of umbilical cord blood (UCB) grafts for hematopoietic stem cell transplantation (HSCT) is a promising technique that permits degree human leukocyte antigen mismatch between the graft and host without concomitant higher rate graft-versus-host disease would be observed an adult marrow mismatched host. A disadvantage to UCB HSCT immune reconstitution may significantly delayed because low dose available in graft. Ex vivo expansion CD34 cells provide greater number cells;...
The fetal liver is the site of a major expansion hematopoietic stem cell (HSC) pool and also privileged organ to study megakaryocyte progenitor differentiation. We identified in mouse at day 13.5 discrete stromal population harboring CD45-TER119-CD31-CD51+VCAM-1+PDGFRα- (V+P-) phenotype that lacked colony-forming unit fibroblast activity harbored an hepatocyte signature. This previously undescribed V+P- efficiently supported production from bone marrow HSC human peripheral blood HSC-myeloid...
Transplantation of BM stromal cells engineered to secrete therapeutic factors could represent a treatment for large array hematologic disorders. The aim this study was evaluate the susceptibility human cell precursors retroviral gene transfer, then ability those be transplanted in vivo. We have transduced recombinant retrovirus encoding mouse CD2 antigen into STRO-1+ selected from adult and fetal BM. Gene-modified were injected intravenously NOD-SCID mice engrafted previously with pieces...
Ex vivo expanded CD34+ progenitor cells from fresh or cryopreserved primate bone marrow, induced to granulocytic differentiation with growth factors, were investigated determine whether myeloid produced in liquid cultures have the normal biologic functions needed for treatment of patients neutropenia following high-dose chemotherapy therapeutic accidental radiation exposure. Human and simian (baboons macaques) cultured granulocyte-colony stimulating factor (G-CSF), stem cell (SCF),...
CD34(+) cell dose provides a measure of hematopoietic tissue that predicts the rate engraftment upon transplant. It is positively correlated with multiple measures recovery, including platelet engraftment. Here we identify subpopulation cells coexpress surface antigen--MA6, which more in clinical setting than alone. The specific identity and function MA6 remain to be determined, however, it expressed by primitive megakaryocyte (MK) progenitors, but lost differentiation not platelets....