A5089011216- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Neuroscience of respiration and sleep
- Obstructive Sleep Apnea Research
- Respiratory Support and Mechanisms
- Histone Deacetylase Inhibitors Research
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Nonmelanoma Skin Cancer Studies
- Cancer Research and Treatments
- Legionella and Acanthamoeba research
- Retinoids in leukemia and cellular processes
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Protein Degradation and Inhibitors
- Mycobacterium research and diagnosis
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- NF-κB Signaling Pathways
- Bone health and treatments
- Skin and Cellular Biology Research
- Sleep and Wakefulness Research
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Radiopharmaceutical Chemistry and Applications
The University of Queensland
2013-2022
University of Dayton
2022
Translational Research Institute
2014-2021
Rheumatology Consultants
2019
Princess Alexandra Hospital
2008-2018
Griffith University
2013
University of Florida
2013
Public Health England
2003-2013
Genomics (United Kingdom)
2004-2011
Wesley Hospital
2010
A method is described for the rapid isolation and purification of bacterial genomic DNA. total 215 strains representing species Campylobacter, Corynebacterium, Escherichia, Legionella, Neisseria, Staphylococcus Streptococcus, were lysed with guanidium thiocyanate. DNA was prepared using just three other reagents one high-speed centrifugation step. The method, which applicable to both Gram-positive Gram-negative bacteria, eliminated endogenous nuclease activity avoided need phenol, RNase...
A common clinical dilemma faced by sleep physicians is in deciding the level of severity at which patients with obstructive apnea (OSA) should be treated. There particular uncertainty about need for, and effectiveness of, treatment mild cases. To help define role nasal continuous positive airway pressure (CPAP) OSA we undertook a randomized controlled cross-over trial CPAP an apnea– hypopnea index (AHI) 5 – 30 (mean, 12.9 ± 6.3 SD). Twenty-four-hour blood neurobehavioral function were...
Polyphasic methods were used to examine the taxonomic positions of three newly identified Grahamella species. A comparison 16S rRNA gene sequences these organisms with available for other bacteria revealed that species form a tight monophyletic cluster members genus Bartonella. This is only remotely related order Rickettsiales. Determinations levels DNA relatedness between and Bartonella (by using modified hydroxyapatite method) all belonging two genera are distinct but closely related. On...
Daytime pulmonary hypertension (PH) is relatively common in obstructive sleep apnea (OSA) and thought to be associated with vascular remodeling (PRm). The extent which PH reversible treatment uncertain. To study this, we measured hemodynamics (Doppler echocardiography) 20 patients OSA (apnea–hypopnea index [AHI] 48.6 ± 5.2/h, mean SEM) before after 1 4 mo of CPAP (compliance 4.7 0.5 h/night). Patients had normal lung function, no cardiac disease or systemic hypertension. Doppler studies were...
We conducted a study of the prevalence sleep-disordered breathing in subjects derived from random sample population. A total 2,202 35 to 69 yr age were approached. Four hundred forty-one answered questionnaire concerning their sleep symptoms, general health, and habits such as alcohol consumption, they monitored for (SDB). The was biased favor snorers those with other subjective complaints. Fifty-six percent men. Of 441 79 (17.9%) had SDB (more than 15 episodes apnea or hypopnea per hour:...
Two sons and their father had severe hypersomnolence obstructive sleep apnea. A third son, although asymptomatic, was shown to have upper-airway obstruction during sleep. Electromyographic recordings of genioglossus activity in the two symptomatic revealed loss tonic early stages at times when apnea occurred. The asymptomatic son showed rapid-eye-movement sleep, period sudden deaths occurred this family. 30-year-old brother died home while asleep, a child age four months from presumed...
Animal studies have shown activation of upper airway muscles prior to inspiratory efforts the diaphragm. To investigate this sequence in humans, we measured electromyogram (EMG) alae nasi (AN) and compared time onset EMG airflow, during wakefulness, stage II or III sleep (3 subj), CO2-induced hyperpnea (6 subj). During interval between AN airflow was 92 +/- 34 ms (mean SE). At a CO2 level greater than equal 43 Torr, 316 38 (P < 0.001). hyperpnea, magnitude increased direct proportion...
The cellular and molecular microenvironment of epithelial stem progenitor cells is poorly characterized despite well-documented roles in homeostatic tissue renewal, wound healing, cancer progression. Here, we demonstrate that, organotypic cocultures, dermal pericytes substantially enhanced the intrinsically low tissue-regenerative capacity human epidermal that have committed to differentiate this enhancement was independent angiogenesis. We used microarray analysis identify genes expressed...
Fifty patients with chronic obstructive lung disease were questioned about their sleep quality and responses compared those of 40 similarly aged without symptomatic disease. Patients reported more difficulty in getting to staying asleep daytime sleepiness than the control group. More twice as many (28%) controls (10%) regular use hypnotics. In a subgroup 16 (mean FEV1 0.88 (SD 0.44) sleep, breathing, oxygenation measured examine relationship between night time hypoxaemia quality. Sleep...
We have investigated the ability of a statistical model developed from clinical data and questionnaire responses to predict disturbance breathing during sleep. Data 100 consecutive patients referred for sleep study suspected apnea were used develop using logistic regression analysis. For each subject, predicted probability having an apnea-hypopnea index (AHI) greater than 15; this was compared with AHI measured study. A cutoff point (= 0.15) decided on that minimized number subjects...
We studied the long-term acceptability of nasal continuous positive airway pressure (CPAP) treatment in 168 consecutive patients, 147 with obstructive sleep apnea (OSA) and 21 snoring. Follow-up was between 1.5 78 months. At latest follow-up 107 (64%) were still using CPAP. Acceptance CPAP least for patients snoring alone (6 persisted) best both excessive daytime somnolence severe hypoxemia (minimum SaO2 < 75%), whom 40 45 (89%) persisted treatment. Patients but without less tolerant (39 71,...
In this study, we have characterized the cDNA clone SQ37 that was isolated previously from a rabbit squamous cell library. The gene encodes 14-kDa protein appears to function as component of cross-linked envelope in differentiating cells. protein, which has been named cornifin, high content proline (31%), glutamine (20%), and cysteine (11%) contains 13 repeats an octapeptide (consensus sequence, EPCQPKVP) at its C terminus. mRNA are induced during differentiation tracheal (RbTE) cells human...
It is controversial whether obstructive sleep apnea (OSA) causes pulmonary hypertension (PH) in the absence of hypoxemic lung disease. To investigate this further we measured awake hemodynamics, gas exchange, and small airways function 32 patients with OSA (apnea- hypopnea index, mean ± SE, 46.2 3.9/h) who had normal screening function. Pulmonary artery pressure (Ppa) cardiac output were by Doppler echocardiography at three levels inspired oxygen (Fi O2 0.50, 0.21, 0.11) during incremental...
Interferon gamma (IFN-gamma) is a potent inducer of squamous differentiation in normal human epidermal keratinocytes. This induction characterized by > or = 95% decrease the mRNA level two growth regulatory genes, cdc2 and E2F-1, 7-15-fold increase expression cell-specific transglutaminase type I cornifin. In contrast to E2F-1 expression, cornifin mRNAs IFN-gamma occurs after lagtime more than 12 h. These results are consistent with hypothesis that keratinocyte cells irreversible arrest...
Mycobacterium tuberculosis isolates from 167 patients attending three London hospitals were analyzed by two techniques for strain differentiation. A significant number of that appeared identical with the recently developed spoligotyping system could be distinguished each other IS6110 restriction fragment length polymorphism analysis, latter technique demonstrating a generally higher level discrimination. Spoligotyping, on hand, was particularly useful analysis low copy numbers, and use in...
Histone deacetylase inhibitors show promise as chemotherapeutic agents and have been demonstrated to block proliferation in a wide range of tumor cell lines. Much this antiproliferative effect has ascribed the up-regulated expression cyclin-dependent kinase inhibitor p21(WAF1/CIP1). In article, we report that p21 was by relatively low doses histone azelaic bishydroxamic acid (ABHA) correlated with proliferative arrest. Higher ABHA were cytotoxic. Cells did not up-regulate hypersensitive...
The 16S ribosomal RNA sequences of Legionella pneumophila, L. erythra, hackeliae, spiritensis, longbeachae, bozemanii (Fluoribacter bozemanae) and micdadei (Tatlockia micdadei) were determined using reverse transcriptase. compared with published for Gram-negative bacteria phylogenetic trees constructed. data confirm previous work which showed that the family Legionellaceae forms a monophyletic subgroup within gamma subdivision Proteobacteria. show all legionellae studied are highly related...