A5055449241- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Protist diversity and phylogeny
- Liver Diseases and Immunity
- Molecular Biology Techniques and Applications
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
Centre National de la Recherche Scientifique
1997-2023
Université Paris Sciences et Lettres
2018-2023
Dynamique de l'information génétique : bases fondamentales et cancer
2011-2023
Unité de Technologies Chimiques et Biologiques pour la Santé
2018-2022
Université Paris Cité
2018-2020
Sorbonne Paris Cité
2018-2020
Inserm
2018-2020
Délégation Paris 5
2018-2020
Sorbonne Université
2006-2019
Sorbonne University Abu Dhabi
2019
Abstract Common fragile sites (CFSs) are chromosome regions prone to breakage upon replication stress known drive rearrangements during oncogenesis. Most CFSs nest in large expressed genes, suggesting that transcription could elicit their instability; however, the underlying mechanisms remain elusive. Genome-wide timing analyses here show stress-induced delayed/under-replication is hallmark of CFSs. Extensive genome-wide nascent transcripts, origin positioning and fork directionality reveal...
Gene therapy is based on the vectorization of genes to target cells and their subsequent expression. Cationic amphiphile-mediated delivery plasmid DNA nonviral gene transfer method most often used. We examined supramolecular structure lipopolyamine/plasmid complexes under various condensing conditions. Plasmid complexation with lipopolyamine micelles whose mean diameter was 5 nm revealed three domains, depending ratio. These domains respectively corresponded negatively, neutrally, positively...
Abstract Hepatic fibrosis is a major consequence of chronic liver disease such as non-alcoholic steatohepatitis which undergoing dramatic evolution given the obesity progression worldwide, and has no treatment to date. stellate cells (HSCs) play key role in process, because damage, they transdifferentiate from “quiescent” an “activated” phenotype responsible for most collagen deposition tissue. Here, using diet-induced murine model (choline-deficient amino acid-defined, high fat diet), we...
Currently, most nonviral nucleic acid vectors are in the form of colloidal suspensions administered primarily parenterally. This type formulation and mode administration impose strong constraints such as size or production sterile preparations. The tablet provides access to easy oral administration, well accepted by patients; As regards vectors, a dry represents an advance terms stability. Using optimized lipid-based small interfering RNA-delivery system, we studied tabletability liquid...
The nature of mammalian origins DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones initiation whereas several other techniques, such as quantitative PCR, disclosed more discrete sites at the same chromosomal loci. In report we analyze an amplified genomic region encompassing 3'-end GNAI3 gene, entire GNAT2 gene intergenic between them in exponentially growing Chinese hamster fibroblasts. These cells...
Summary Genome integrity requires replication to be completed before chromosome segregation. This coordination essentially relies on replication-dependent activation of a dedicated checkpoint that inhibits CDK1, delaying mitotic onset. Under-replication Common Fragile Sites (CFSs) however escapes surveillance, which triggers breakage. Using human cells, we asked here whether such leakage results from insufficient CDK1 inhibition under modest stresses used destabilize CFSs. We found tight...
Molecular combing technology is an important new tool for the functional and physical mapping of genome segments. It designed to identify amplifications, microdeletions, rearrangements in a DNA sequence study process replication. This technique has recently been used analyze dynamics replication amplified domains. In Bradysia hygida, multiple amplification initiation sites are predicted exist upstream BhC4-1 gene. However, it impossible them using available standard techniques. The aim this...
ABSTRACT Common Fragile Sites (CFSs) are chromosome regions prone to breakage under replication stress, known drive rearrangements during oncogenesis. Most CFSs nest in large expressed genes, suggesting that transcription elicits their instability but the underlying mechanisms remained elusive. Analyses of genome-wide timing human lymphoblasts here show stress-induced delayed/under-replication is hallmark CFSs. Extensive analyses nascent transcripts, origin positioning and fork...