A5003572804- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- Protist diversity and phylogeny
- Computational Geometry and Mesh Generation
- Graph Theory and Algorithms
- Ubiquitin and proteasome pathways
- Genetic diversity and population structure
- Marine Biology and Ecology Research
Sorbonne Université
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Institut Curie
2019-2024
Laboratoire de Biologie du Développement de Villefranche-sur-Mer
2020-2024
Institut de la Mer de Villefranche
2024
Université Paris Sciences et Lettres
2019-2023
Dynamique de l'information génétique : bases fondamentales et cancer
2023
Université Paris 1 Panthéon-Sorbonne
2023
Sorbonne University Abu Dhabi
2019
Institut de Biologie Intégrative de la Cellule
2019
Abstract Common fragile sites (CFSs) are chromosome regions prone to breakage upon replication stress known drive rearrangements during oncogenesis. Most CFSs nest in large expressed genes, suggesting that transcription could elicit their instability; however, the underlying mechanisms remain elusive. Genome-wide timing analyses here show stress-induced delayed/under-replication is hallmark of CFSs. Extensive genome-wide nascent transcripts, origin positioning and fork directionality reveal...
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by mutations in the FMR1 gene and deficiency of functional FMRP protein. known as translation repressor whose nuclear function not understood. We investigated global impact on genome stability due to loss. Using Break-seq, we map spontaneous replication stress-induced DNA double-strand breaks (DSBs) an FXS patient-derived cell line. report that genomes cells are inherently unstable accumulate twice many DSBs those from...
Abstract Macrosynteny refers to the conservation of chromosomal sub-chromosomal domains across species and its can provide insight on evolution animal genomes. Pairwise comparison de-novo assembled genomes based predicted protein sequences often use a graphical visualization called an Oxford grid. We implemented R package draw grids from standard genomic file formats. The automatically order chromosomes, improve interpretability, is thus helpful for both exploratory data analysis production...
Summary Replication stress, a major hallmark of cancers, and ensuing genome instability source from impaired progression replication forks. The first line defense against fork slowing is compensation, long-described process that elicits firing otherwise dormant origins. It remains unclear whether compensation requires activation the DNA checkpoint or passively results lengthening window time during which origins can fire when slows, both. Using molecular combing we show here linear...
Abstract The types of genomic change needed for environmental adaptation are great interest. Annelid worms a large phylum found in rich diversity habitats, giving opportunities to explore this issue. We report the chromosome level genome sequence Pompeii worm, annelid Alvinella pompejana , an inhabitant extreme deep-sea hydrothermal vent environment. find strong but heterogeneously distributed genetic divergence between populations taken from either side equator. Using transcript data, we...
ABSTRACT Fragile X syndrome (FXS) is the most prevalent inherited intellectual disability caused by mutations in Mental Retardation 1 ( FMR1 ) gene. The protein product of , FMRP, known as a translational repressor whose nuclear function not understood. Here we report that FMRP genome maintenance protein. We show FX cells exhibit elevated level chromosome breaks, both spontaneous and replication stress-induced. demonstrate required for abating R-loop accumulation, thereby preventing...
Summary Genome integrity requires replication to be completed before chromosome segregation. This coordination essentially relies on replication-dependent activation of a dedicated checkpoint that inhibits CDK1, delaying mitotic onset. Under-replication Common Fragile Sites (CFSs) however escapes surveillance, which triggers breakage. Using human cells, we asked here whether such leakage results from insufficient CDK1 inhibition under modest stresses used destabilize CFSs. We found tight...
<title>Abstract</title> Replication stress, a major hallmark of cancers, sources from replication fork slowing or blocking. In response, activation extra-origins, otherwise dormant, supports the rate. Whether DNA checkpoint drives this compensation process remained unclear. Here, combing analyses show that linear relationship ties inter-origin distances to speeds and slope line, called stressline, accurately quantifies efficiency. Comparison slopes in human cells with different genotypes...
ABSTRACT Common Fragile Sites (CFSs) are chromosome regions prone to breakage under replication stress, known drive rearrangements during oncogenesis. Most CFSs nest in large expressed genes, suggesting that transcription elicits their instability but the underlying mechanisms remained elusive. Analyses of genome-wide timing human lymphoblasts here show stress-induced delayed/under-replication is hallmark CFSs. Extensive analyses nascent transcripts, origin positioning and fork...