Abstract Background Cellular miRNAs play an important role in the regulation of gene expression eukaryotes. Recently, have also been shown to be able target and inhibit viral expression. Computational predictions revealed earlier that HIV-1 genome includes regions may potentially targeted by human miRNAs. Here we report functionality predicted miR-29a site nef gene. Results We find hsa-miR-29a 29b are expressed peripheral blood mononuclear cells. Expression a luciferase reporter bearing was...

Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. mRNA known to be localized neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3′-untranslated region (UTR) not characterized there are no experimentally determined polyadenylation (polyA) sites actin mRNA. We have found that the cytoplasmic β-actin (Actb) gene generates two alternative transcripts terminated at tandem...

MicroRNAs (miRNAs) are small, endogenous, regulatory RNA molecules that can bind to partially complementary regions on target messenger RNAs and impede their expression or translation. We rationalized miRNAs, being localized the cytoplasm, will be maternally inherited during fertilization may play a role in early development. Although Dicer is known essential for transition from single-celled zygote two-cell embryo, direct miRNAs has not yet been demonstrated. identified with targets...

Stem cells are the key cellular source for regenerating tissues and organs in vertebratespecies. Historically, investigation of stem cell fate decisions vivo has been assessed intissue sections using immunohistochemistry (IHC), where a trained user quantifies fluorescentsignal multiple randomly selected images manual counting—which is prone toinaccuracies, bias, very labor intensive. Here, we highlight performance recentlydeveloped machine-learning (ML)-based image analysis program called...

This study aimed to investigate the role of miRNAs in HPV-mediated cervical pre-cancer and cancer cases Indian population. We analysed HPV infection its genotypes uterine (n = 80), 200) normal samples 150) by consensus sequence PCR followed type specific PCRs. Also, microRNA profiling was done a subset 20), 50) 30) real-time quantitative (qRT-PCR). The prevalence found be 81 % (65/80) 94 (188/200) cases, with most predominant high-risk type-16 (HR-HPV-16) 83 91 pre- respectively. Whereas...

Abstract HIV-1 replication inside host cells is known to be regulated by various factors. Host miRNAs, virtue of its normal functioning, also regulate RNA expression either directly targeting virus mRNAs or indirectly regulating proteins that uses for own replication. Therefore, it highly possible with differential miRNA expression, rate disease progression will vary in infected individuals. In this study we have compared a panel 13 reported anti-HIV miRNAs human PBMCs from long term non...

Abstract Background The AP-1 transcription factor, FBJ osteosarcoma oncogene (FOS), is induced in adult muscle satellite cells (SCs) within hours following damage and required for effective stem cell activation repair. However, why FOS rapidly downregulated before SCs enter cycle as progenitor (i.e., transiently expressed) remains unclear. Further, whether boosting levels the proliferating progeny of can enhance their myogenic properties needs further evaluation. Methods We established an...

Thioacetamido nucleic acids (TANA) contain a backbone modification of dinucleotides consisting 5-atom amide linker N3′-COCH2-S-CH2 at thymidine or thymidine–cytidine dimer blocks. Here, the chemical synthesis TANA linked 5-methyl-cytidine–cytidine (Mecc) block and its incorporation into DNA sequence, complementary to human microRNA 34 (miR-34) is described. Further, for first time, we demonstrate biological applications modified oligonucleotides in detection intracellular knockdown cancer...

We had previously proposed that the post-transcriptional regulation through microRNA as a mechanism for incomplete penetrance and variable expressivity, leads to lack of correlation between genotype phenotype. Here we report validation miRNA-target interactions predicted earlier demonstrate endogenous JAG1 by hsa-miR-214 hsa-miR-124, TGFBR2 hsa-miR-34b*, luciferase activity reporter constructs also expression levels genes. Using these targets, have modeled diploid state miRNA target site...

Cancer is a medical condition in which cells divide abnormally the human body and spread to other parts. If prognosis of cancer done well advance, it curable. It estimated that 22.2 million individuals would be impacted by between now 2030. There are about 100 different forms cancer, categorized based on criteria including age, gender, race/ethnicity. organs such as lung, liver, kidney, breast, brain was investigated present study. The data for Lung study came from Kaggle online system, TCIA...

General stores, once the backbone of local economies, now confront a plethora challenges in today's rapidly evolving retail landscape. This abstract explores multifaceted hurdles faced by general store businesses and offers strategic insights to navigate them effectively. Evolving consumer preferences represent significant challenge, as shoppers increasingly prioritize convenience, sustainability, value. Moreover, rise e-commerce giants intensifies competition, compelling stores...

In today's Industry 4.0 landscape, dynamic inventory management stands as a cornerstone for businesses seeking to navigate the complexities of modern supply chains. Leveraging transformative potential Artificial Intelligence (AI) and Robotics, companies can revolutionize their strategies achieve unprecedented levels agility responsiveness. AI algorithms enable precise demand forecasting, allowing organizations optimize in real time, thus mitigating risks associated with stockouts or excess...

In the digital age, process of making career choices has been significantly influenced by proliferation online reviews and rise social media influencers. This literature review explores multifaceted role these phenomena play in shaping decisions. Online on platforms like Glassdoor LinkedIn provide job seekers with critical insights into company cultures, employee experiences, advancement opportunities, empowering them to make informed Concurrently, influencers leverage their offer...

The evolving landscape of marketing presents significant opportunities and challenges for businesses aiming to reach both urban rural audiences. While digital has gained prominence with the rise internet penetration mobile usage, print media continues hold relevance, especially in regions. This paper explores integration design effective, targeted strategies that cater unique needs behaviours consumers areas. Through a mixed-methods approach, this study examines how these two forms can...

This study explores the relationship between work-life balance and job satisfaction among employees in cement industry of Vindhya region. Employing a mixed-methods approach, research delves into factors that influence employees' ability to maintain harmonious their professional responsibilities personal lives, how these impact overall satisfaction. Data was collected through comprehensive surveys in-depth interviews with across various levels industry.The findings indicate while competitive...

This paper examines the shift in clothing shopping preferences from offline to online platforms within Vindhya region. With rapid expansion of e-commerce and changing consumer behaviors, understanding regional variances has become increasingly crucial. study investigates factors influencing choices between shopping, focusing on convenience, pricing, variety, local cultural factors. Utilizing both quantitative surveys qualitative interviews, research highlights evolving landscape retail The...

Stem cells are the key cellular source for regenerating tissues and organs in vertebrate species. Historically, investigation of stem cell fate decisions vivo has been assessed tissue sections using immunohistochemistry (IHC), where a trained user quantifies fluorescent signal multiple randomly selected images manual counting. This approach is prone to inaccuracies, bias, very labor intensive. Here, we highlight performance recently developed machine-learning (ML)-based image analysis...

This study investigates the impact of advertising and outdoor media on Prism Cement Limited's market performance in Satna. The research employs both qualitative quantitative methods to analyze how strategies campaigns contribute brand visibility, customer engagement, share for Limited. Through surveys, interviews, financial analysis, aims uncover correlations between expenditures, channels utilized, indicators such as sales growth recognition. findings suggest significant insights into...

In the contemporary tourism industry, efficient logistics and supply chain practices play a crucial role in enhancing visitor experiences ensuring sustainable growth. This paper explores impact of optimized logistical operations robust management on sector, highlighting how these contribute to improved service quality, reduced operational costs, increased customer satisfaction. Through comprehensive review existing literature case studies from diverse geographical regions, this research...

The rapid advancement of digital technologies has transformed marketing strategies globally, offering businesses unprecedented opportunities to reach their target audiences. However, the adoption in rural areas presents unique challenges that are often underestimated. This paper delves into critical barriers face when attempting implement regions. Key identified include limited internet access, low levels literacy, and resistance change rooted cultural differences. Additionally, lack...

Optimizing supply chain performance has become increasingly critical in today's competitive global market. This paper investigates the synergistic effects of effective marketing and logistics coordination on performance. The study proposes a conceptual framework that integrates strategies logistical operations to enhance efficiency, responsiveness, overall metrics within chains. By analyzing case studies empirical data, this research demonstrates how coordinated efforts between can lead...

In the face of growing climate change awareness, consumer preferences are increasingly shifting toward eco-friendly products and services. This paper presents a theoretical analysis how environmental consciousness impacts behaviour shapes demand for sustainable alternatives. Drawing upon established models marketing theories, examines role strategies in influencing purchasing decisions. The explores effectiveness green marketing, corporate social responsibility (CSR), sustainability branding...

Background The events during host virus interaction pave the way to differential outcomes in HIV-1 disease progression rates. We have earlier shown that HIV-1Nef gene harbors target sites for human microRNA, miRNA 29a/b. Over expression of miR 29a/b could reduce levels by targeting Nef gene. hypothesized microRNA maybe a protective factor AIDS progression.