A5007761510- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- Insect Resistance and Genetics
- Research on scale insects
- Genetics, Bioinformatics, and Biomedical Research
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Plant Physiology and Cultivation Studies
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Chromatin Remodeling and Cancer
- Pharmacogenetics and Drug Metabolism
- Immunodeficiency and Autoimmune Disorders
- Drug Transport and Resistance Mechanisms
- Folate and B Vitamins Research
- Histone Deacetylase Inhibitors Research
Ambedkar University Delhi
2013-2022
University of Delhi
2013-2022
Stockholm University
2020
Motamed Cancer Institute
2020
Academic Center for Education, Culture and Research
2020
Indian Institute of Science Bangalore
1984-2013
Centre for Cellular and Molecular Biology
2008
Institute of Genomics and Integrative Biology
2008
The University of Texas MD Anderson Cancer Center
2008
University of Milan
2008
Epigenetic modifications, especially alteration in DNA methylation, are increasingly being recognized as a key factor the pathogenesis of complex disorders, including atherosclerosis. However, there limited data on epigenetic changes coronary artery disease (CAD) patients. In present study we evaluated methylation status genomic from peripheral lymphocytes cohort 287 individuals: 137 angiographically confirmed CAD patients and 150 controls. The differential susceptibility to...
Abstract Background The mce4 operon is one of the four homologues mammalian cell entry ( mce ) operons Mycobacterium tuberculosis . mce4A (Rv3499c) gene within this homologous to mce1A (Rv0169), that has a role in host invasion by M. Our earlier reports show expressed during stationary phase growth bacillus culture and course infection hosts. carrying mutation shows defect reduced survival infected mice. However, intracellular localization Mce4A protein its direct or not been demonstrated so...
Abstract Background Global regulatory mechanisms involving chromatin assembly and remodelling in the promoter regions of genes is implicated eukaryotic transcription control especially for subjected to spatial temporal regulation. The potential utilise global controlling gene expression might depend upon architecture around gene. In-silico analysis can yield important insights into this aspect, facilitating comparison two or more classes comprising a large number within each group. Results...
The coccid insects (Hemiptera; Sternorrhyncha; Aphidiformes; Coccoidea; Pseudococcidae) are well suited to study not only the mechanisms of genomic imprinting but also facultative heterochromatization, a phenomenon exemplified by inactivation X chromosome in female mammals. Coccids show sex-specific heterochromatization an entire set chromosomes and transcriptional silencing all paternally contributed males. Thus, resultant differential regulation operate on 50% genome contrast single A...
Nucleosome positioning maps of several organisms have shown that Transcription Start Sites (TSSs) are marked by nucleosome depleted regions flanked strongly positioned nucleosomes. Using genome-wide and histone variant occupancy in the mouse liver, we show majority genes were associated with a single prominent H2A.Z containing their promoter region. We classified into clusters depending on proximity to TSS. The no detectable showed lowest expression level, whereas was closer TSS higher...
The sequencing of the complete genome M. tuberculosis H37Rv has resulted in recognition four mce operons its by silico analysis. In an attempt to understand significance redundancy operons, we analyzed expression profile after different periods growth culture as well during vivo infection. Our results strongly suggest that mce1 is expressed a polycistronic message. from day 8 12, only was observed, but cultures progress towards stationary phase altered; transcripts operon were barely...
Mammalian cell entry (mce) operons, implicated in the of mycobacteria into host cells, are present pathogenic and saprophytic species. It is likely that genes these operons have functions other than those required for cells. Using silico analysis we identified domains within mce might justify their occurrence species like Mycobacterium smegmatis. Our addition to domain, presence Ttg2B Ttg2C domains, typical proteins involved transport. We also analysed compared expression profile between...
Abstract Incomplete penetrance and variable expressivity are non‐Mendelian phenomena resulting in the lack of correlation between genotype phenotype. Not withstanding diversity mechanisms, differential expression homologous alleles within cells manifests as variations mutations individuals same genotype. These seen most often dominantly inherited diseases, implying that they sensitive to concentration gene product. In this framework advances understanding role microRNA (miRNA) fine‐tuning at...
The homologues of yeast INO80 are identified across phyla from Caenorhabditis elegans to human. In Drosophila it has been shown that dINO80 forms a complex with Pleiohomeotic but does not interact Hox PRE (polycomb responsive element). As some proteins the implicated in homeotic gene regulation, we examined if is involved regulation genes. We find null mutants generated by imprecise excision P‐element late embryonic lethals and show transformation. detect misexpression genes like Sex‐comb...
Epigenetic cellular memory mechanisms that involve polycomb and trithorax group of proteins are well conserved across metazoans. The cis-acting elements interacting with these proteins, however, poorly understood in mammals. In a directed search we identified potential responsive element 25 repeats YY1 binding motifthatwe designate PRE-PIK3C2B as it occurs the first intron human PIK3C2B gene. It down regulates reporter gene expression HEK cells repression is dependent on (PcG). We...
The presence of a highly conserved DNA binding domain in INO80 subfamily predicted that directly interacts with and we demonstrated its activity vitro. Here report the consensus motif recognized by DBINO identified SELEX method demonstrate specific interaction motif. We show significantly down regulates reporter gene expression through motif, repression is dependent on but not YY1 cell. lost if residues within are altered. identify large number potential target sites human genome silico...
The alternative sigma factors are regulated by a phosphorylation‐mediated signal transduction cascade involving anti‐sigma and anti‐anti‐sigma factors. proteins regulating Mycobacterium tuberculosis factor F (SigF), anti‐SigF anti‐anti‐SigF have been identified, but the catalyzing phosphorylation–dephosphorylation not well established. We identified distinct pathogenic species‐specific multidomain protein, Rv1364c, in which components of entire for SigF regulation appear to be encoded single...
Abstract Background The mce operons play an important role in the entry of M. tuberculosis into macrophages and non-phagocytic cells. Their non-redundant function as well complex regulation is implied by phenotype mutants. Recently, mce1 operon was found to extend over 13 genes, fadD5 (Rv0166) being first gene operon. presence a non-coding sequence 200 base pairs between Rv0166 Rv0167 peculiar among four M.tuberculosis . We have examined this region. Results predicted putative promoter...
Abstract Background The presence of four mammalian cell entry ( mce ) operons in Mycobacterium tuberculosis suggests the essentiality functions genes these operons. differential expression different phases vitro growth and infected animals reported earlier from our laboratory further justifies apparent redundancy for genome. Here we investigate extent polymorphism eight mce1 mce4 M. standard reference strains (H37Rv, H37Ra, LVS (Low Virulent Strain) BCG) 112 clinical isolates varying their...