A5022713360- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Chemical synthesis and alkaloids
- Cholinesterase and Neurodegenerative Diseases
- Phosphodiesterase function and regulation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Chromosomal and Genetic Variations
- Pharmaceutical and Antibiotic Environmental Impacts
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Virology and Viral Diseases
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Cancer-related gene regulation
University of Zurich
2020-2024
Canadian Food Inspection Agency
2019
Institute of Genomics and Integrative Biology
2013
Council of Scientific and Industrial Research
2013
University of Delhi
2012
Industrial Research Institute
2012
Abstract Eukaryotic chromosomes are folded into hierarchical domains, forming functional compartments. Nuclear periphery and nucleolus two nuclear landmarks contributing to repressive chromosome architecture. However, while the role of lamina (NL) in genome organization has been well documented, function remains under-investigated due lack methods for identification nucleolar associated domains (NADs). Here we have established DamID- HiC-based methodologies generate accurate genome-wide maps...
MicroRNAs (miRNAs) are short regulatory RNA molecules that interfere with the expression of target mRNA by binding to complementary sequences. Currently, most common method for identification targets miRNAs is computational prediction based on free energy change calculations, site accessibility and conservation. Such algorithms predict hundreds each miRNA, necessitating tedious experimentation identify few functional targets. Here we explore utility miRNA-proteomics as an approach...
Nucleosome positioning maps of several organisms have shown that Transcription Start Sites (TSSs) are marked by nucleosome depleted regions flanked strongly positioned nucleosomes. Using genome-wide and histone variant occupancy in the mouse liver, we show majority genes were associated with a single prominent H2A.Z containing their promoter region. We classified into clusters depending on proximity to TSS. The no detectable showed lowest expression level, whereas was closer TSS higher...
Abstract Eukaryotic chromosomes are folded into hierarchical domains, enabling the organization of genome functional compartments. Nuclear periphery and nucleolus two nuclear landmarks thought to contribute repressive chromosome architecture. However, while role lamina (NL) in has been well documented, function remains under-investigated due lack methods for genome-wide maps nucleolar associated domains (NADs). Here we established a method based on Dam-fused engineered histone H2B that marks...
Thioacetamido nucleic acids (TANA) contain a backbone modification of dinucleotides consisting 5-atom amide linker N3′-COCH2-S-CH2 at thymidine or thymidine–cytidine dimer blocks. Here, the chemical synthesis TANA linked 5-methyl-cytidine–cytidine (Mecc) block and its incorporation into DNA sequence, complementary to human microRNA 34 (miR-34) is described. Further, for first time, we demonstrate biological applications modified oligonucleotides in detection intracellular knockdown cancer...
Phosphodiesterase 4B (PDE4B) is an important therapeutic target for asthma and chronic obstructive pulmonary disease. To identify PDE4 subtype-specific compounds using high-throughput assays, full-length recombinant proteins are needed in bulk quantity. In the present study, human PDE4B2 was expressed cellular slime mould Dictyostelium discoideum (Dd). A cell density of 2 x 10(7) cells/mL obtained up to 1 mg/L purified through Ni-NTA affinity chromatography. The protein soluble its activity...
Complete genome sequences of six Ambystoma tigrinum viruses (ATV) were determined directly from tail clips western tiger salamanders (Ambystoma mavortium) 2013 (high-mortality year) and 2014 (low-mortality in Alberta, Canada. The lengths ranged 106,258 to 106,915 bp contained 108 open reading frames encoding predicted proteins larger than 50 amino acids.
Abstract Gene position in the nuclear space plays an important role gene regulation. This is exemplified by repressive chromatin domains frequently contacting lamina or nucleoli. The nucleolus undergoes structural changes response to various cellular states, potentially impacting genome organization. However, how 3D-genome organization responds nucleolar states has remained underinvestigated due lack of methods able identify associated (NADs) single cells and under stress. To address this,...
Abstract Repressive chromatin domains are often located at the nuclear lamina (NL) or nucleolus. Although nucleolar associated (NADs) have been recently mapped, mechanisms of NAD association with nucleoli and functional significance their localization remain unclear. Here, we show that is mediated by nucleophosmin (NPM1), a factor within granular component, outer layer NPM1 binds NADs, interacts histone lysine methyltransferase G9a (EHMT2), required for establishing H3K9me2 NADs. Loss...