A5102943424- Fungal and yeast genetics research
- Crop Yield and Soil Fertility
- Biofuel production and bioconversion
- Nuclear Structure and Function
- Fungal Biology and Applications
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Trypanosoma species research and implications
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Antifungal resistance and susceptibility
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Plant Pathogenic Bacteria Studies
- Metal Extraction and Bioleaching
- Minerals Flotation and Separation Techniques
- Studies on Chitinases and Chitosanases
- Vector-Borne Animal Diseases
- Muscle Physiology and Disorders
- Fungal Infections and Studies
- Polysaccharides and Plant Cell Walls
- Corrosion Behavior and Inhibition
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
Universidade Estadual Paulista (Unesp)
2014-2024
Centro Universitário de Araraquara
2010-2024
Universidade de São Paulo
1981-2010
University of Modena and Reggio Emilia
2002
University of Catania
2000
Biotechnology Research Institute
1994-1995
Cereal Research Centre
1991
Aspergillus fumigatus is a primary and opportunistic pathogen, as well major allergen, of mammals. The Ca+2-calcineurin pathway affects virulence, morphogenesis antifungal drug action in A. fumigatus. Here, we investigated three components the pathway, pmcA,-B, -C, which encode calcium transporters. We demonstrated that CrzA can directly control mRNA accumulation pmcA-C genes by binding to their promoter regions. CrzA-binding experiments suggested 5′-CACAGCCAC-3′ 5′-CCCTGCCCC-3′ sequences...
Aspergillus fumigatus is an opportunistic fungal pathogen that causes invasive aspergillosis (IA), a life-threatening disease in immunocompromised humans. The echinocandin caspofungin, adopted as second-line therapy combating IA, β-1,3-glucan synthase inhibitor, which, when used high concentrations, reverts the anticipated A. growth inhibition, phenomenon called "caspofungin paradoxical effect" (CPE). CPE has been widely associated with increased chitin content cell wall due to compensatory...
Small heat shock proteins (sHsps) are ubiquitous low-molecular-weight chaperones that prevent protein aggregation under cellular stresses. sHsps contain a structurally conserved α-crystallin domain (ACD) of about 100 amino acid residues flanked by varied N- and C-terminal extensions usually exist as oligomers. Oligomerization is important for the biological functions most sHsps. However, active oligomeric states not defined yet. We present here crystal structures (up to 1.65 Å resolution)...
Aspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling essential for A. pathogenicity regulated by the CrzA transcription factor. We used ChIP-seq (Chromatin Immunoprecipitation DNA sequencing) to explore gene targets in fumigatus. In total, 165 potential binding peaks including 102 directly genes were identified, resulting prediction A[GT][CG]CA[AC][AG] CrzA-binding motif. The putatively exhibited a diverse array functions. phkB (Afu3g12530) histidine...
Xanthomonas axonopodis pv. citri (Xac) causes citrus canker and the completion of Xac genome sequence has opened up possibility investigating basic cellular mechanisms at genomic level. Copper compounds have been extensively used in agriculture to control plant diseases. The copA copB genes, identified by annotation genome, encode homologues proteins involved copper resistance. A gene expression assay Northern blotting revealed that are expressed as a unique transcript specifically induced...
The fungus Geotrichum candidum produces extracellular lipases. Purification and characterization of different lipase isoforms from various G. strains is difficult due to the close physical biochemical properties isoforms. Consequently, these enzymes their substrate specificites has been difficult. We have determined genes present in four (ATCC 34614, NRCC 205002, NRRL Y‐552 Y‐553) by molecular cloning DNA sequencing. Each strain contains two similar previously identified I II cDNAs. Our data...
Glycogen is a polysaccharide widely distributed in microorganisms and animal cells its metabolism under intricate regulation. Its accumulation specific situation results from the balance between glycogen synthase phosphorylase activities that control synthesis degradation, respectively. These enzymes are highly regulated at transcriptional post-translational levels. The existence of DNA motif for Aspergillus nidulans pH responsive transcription factor PacC promoter gene encoding (gsn)...
Leptin acts as satiety factor and increases energy expenditure. Studies conducted on animals in vitro adipocytes culture have shown that infusion of catecholamines leads to a significant reduction ob gene expression; it appears interest evaluate the vivo effects adrenergic activation expression humans.
The cAMP–PKA signaling pathway plays an important role in many biological processes including glycogen metabolism. In this work we investigated its the Neurospora crassa metabolism control using mutant strains affected components of pathway, cr-1 strain deficient adenylyl cyclase activity therefore has PKA not active, and mcb a temperature-sensitive defective regulatory subunit is with constitutively active PKA. We analyzed expression gene encoding synthase (gsn), enzyme synthesis as...
Despite tremendous progress in the elucidation of three-dimensional structures lipases, molecular basis for their observed substrate preference is not well understood. In an effort to correlate lipase structure with its and clarify contradicting reports literature, we have compared enzymic characteristics two closely related recombinant lipases from fungus Geotrichum candidum. These enzymes were expressed yeast Saccharomyces cerevisiae as fusions N-terminal poly(His) tag purified a single...
Abstract When exposed to stress conditions, all cells induce mechanisms resulting in an attempt adapt that involve proteins which, once activated, trigger cell responses by modulating specific signaling pathways. In this work, using a combination of pulldown assays and mass spectrometry analyses, we identified the Neurospora crassa SEB-1 transcription factor binds Stress Response Element (STRE) under heat stress. Orthologs have been functionally characterized few filamentous fungi as being...
Neurospora crassa is a filamentous fungus that has been extensively studied as model organism for eukaryotic biology, providing fundamental insights into cellular processes such cell signaling, growth and differentiation. To advance in the study of this multicellular organism, an understanding specific mechanisms protein transport nucleus essential. Importin-α (Imp-α) receptor cargo proteins contain nuclear localization signals (NLSs) play key role classical import pathway. Structures Imp-α...
Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species Xanthomonas citri ssp. (XAC), which attacks wide range of citrus hosts. Differential proteomic analysis periplasm-enriched fraction was performed for XAC cells grown in pathogenicity-inducing (XAM-M) and pathogenicity-non-inducing (nutrient broth) media using two-dimensional electrophoresis combined with liquid chromatography-tandem mass spectrometry. Amongst 40 proteins...
Glycogen and trehalose are storage carbohydrates their levels in microorganisms vary according to environmental conditions. In Neurospora crassa, alkaline pH stress highly influences glycogen levels, Saccharomyces cerevisiae, the response also involves calcineurin signaling pathway mediated by Crz1 transcription factor. Recently, yeast, genes were identified as targets of including involved metabolism. this work, we present evidence that N. crassa metabolism is modulated calcium stresses.We...
Yeast strains capable of fermenting starch and dextrin to ethanol were isolated from samples collected Brazilian factories in which cassava flour is produced. Considerable alcohol production was observed for all the selected. One strain (DI-10) fermented rapidly secreted 5 times as much amylolytic enzyme than that Schwanniomyces alluvius UCD 54-83. This three other similar isolates classified Saccharomyces cerevisiae var. diastaticus by morphological physiological characteristics molecular taxonomy.
Significance Circadian rhythms enable organisms to anticipate daily environmental cycles and control the timing of numerous biological processes, including metabolism, optimize health survival organisms. Glycogen metabolism is a conserved glucose homeostatic process; however, molecular mechanisms linking circadian clock glycogen remain largely unknown. In this report, we demonstrate that clock-dependent transcriptional regulation synthase, gsn , regulates oscillations GSN protein...