A5004204876- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Fungal Infections and Studies
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Nail Diseases and Treatments
- Studies on Chitinases and Chitosanases
- Antimicrobial Peptides and Activities
- Plant Disease Resistance and Genetics
- Bacterial biofilms and quorum sensing
- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Sugarcane Cultivation and Processing
- CAR-T cell therapy research
- Infectious Diseases and Mycology
- Protein Tyrosine Phosphatases
- Plant-Microbe Interactions and Immunity
- Alkaline Phosphatase Research Studies
- Polysaccharides and Plant Cell Walls
- Plant Virus Research Studies
Universidade de São Paulo
2016-2025
National Institute of Science and Technology
2025
Universidade de Ribeirão Preto
2015-2024
Universidade Estadual Paulista (Unesp)
2021-2022
Instituto de Ciências Farmacêuticas
2021
High Throughput Biology (United States)
2015
Johns Hopkins University
2015
Universidade Federal do Pará
2015
Aspergillus fumigatus is a primary and opportunistic pathogen, as well major allergen, of mammals. The Ca+2-calcineurin pathway affects virulence, morphogenesis antifungal drug action in A. fumigatus. Here, we investigated three components the pathway, pmcA,-B, -C, which encode calcium transporters. We demonstrated that CrzA can directly control mRNA accumulation pmcA-C genes by binding to their promoter regions. CrzA-binding experiments suggested 5′-CACAGCCAC-3′ 5′-CCCTGCCCC-3′ sequences...
Here, we investigated which stress responses were influenced by the MpkC and SakA mitogen-activated protein kinases of high-osmolarity glycerol (HOG) pathway in fungal pathogen Aspergillus fumigatus. The ΔsakA double ΔmpkC mutants more sensitive to osmotic oxidative stresses, cell wall damaging agents. Both MpkC::GFP SakA::GFP translocated nucleus upon damage, with showing a quicker response. phosphorylation state MpkA was determined post exposure high concentrations congo red Sorbitol. In...
Abstract Fungal infections cause more than 1.5 million deaths a year. Due to emerging antifungal drug resistance, novel strategies are urgently needed combat life-threatening fungal diseases. Here, we identify the host defense peptide mimetic, brilacidin (BRI) as synergizer with caspofungin (CAS) against CAS-sensitive and CAS-resistant isolates of Aspergillus fumigatus, Candida albicans , C. auris CAS-intrinsically resistant Cryptococcus neoformans . BRI also potentiates azoles A. fumigatus...
Abstract Aspergillus fumigatus is a saprophytic fungus that can cause variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production important for its virulence and must be tightly regulated to avoid excess toxicity the fungus. GT self-protection by GliT oxidoreductase GtmA methyltransferase activities related subcellular localization these enzymes how sequestered from cytoplasm increased cell damage. Here, we show GliT:GFP GtmA:GFP are localized in vacuoles during...
The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol. main component cellulose is glucose, whereas hemicelluloses mainly consist pentose sugars such as D-xylose L-arabinose. genomes filamentous fungi A. nidulans encode a multiplicity sugar transporters with broad affinities for hexose sugars. Saccharomyces cerevisiae, which has long history...
The conversion of lignocellulosic biomass to biofuels (second-generation biofuel production) is an environmentally friendlier alternative petroleum-based energy sources. Enzymatic deconstruction lignocellulose, catalyzed by filamentous fungi such as Aspergillus nidulans, releases a mixture mono- and polysaccharides, including hexose (glucose) pentose (xylose) sugars, cellodextrins (cellobiose), xylooligosaccharides (xylobiose). These sugars can subsequently be fermented yeast cells ethanol....
Trichoderma reesei is a saprophytic fungus implicated in the degradation of polysaccharides present cell wall plants. T. has been recognized as most important industrial that secretes and produces cellulase enzymes are employed production second generation bioethanol. A few molecular mechanisms involved process biomass deconstruction by reesei; particular, effect sugar transporters induction xylanases cellulases expression yet to be known. In our study, we characterized novel transporter,...
The production of bioethanol from lignocellulosic feedstocks is dependent on biomass degradation by hydrolytic enzymes. main component lignocellulose cellulose and different types organisms are able to secrete cellulases. filamentous fungus Aspergillus nidulans serves as a model organism study cellulase the available tools allow exploring more in depth mechanisms governing carbon catabolite repression.In A. nidulans, microarray data identified cAMP-dependent protein kinase A (PkaA) being...
Invasive aspergillosis is predominantly caused by Aspergillus fumigatus, and adaptations to stresses experienced within the human host are a prerequisite for survival virulence strategies of pathogen. The central signal transduction pathway operating during hyperosmotic stress high osmolarity glycerol mitogen-activated protein kinase cascade. A. fumigatus MpkC SakA, orthologues Saccharomyces cerevisiae Hog1p, constitute primary regulator response. We compared wild-type transcriptional...
Aspergillus fumigatus is a fungal pathogen that capable of adapting to different host niches and avoid defenses. An enhanced understanding how, which, A. signal transduction pathways are engaged in the regulation these processes essential for development improved disease control strategies. Protein phosphatases central numerous pathways. To comprehend functions protein fumigatus, 32 phosphatase catalytic subunit encoding genes were identified. We have recognized PtcB as one involved high...
The pathogenic fungus Aspergillus fumigatus is able to adapt extremely variable environmental conditions. A. genome contains four genes coding for mitogen-activated protein kinases (MAPKs), which are important regulatory knots involved in diverse cellular responses. From a clinical perspective, MAPK activity has been connected salvage pathways, can determine the failure of effective treatment invasive mycoses using antifungal drugs. Here, we report characterization Saccharomyces cerevisiae...
To characterize the mechanisms involved in glucose transport, filamentous fungus Aspergillus nidulans, we have identified four transporter encoding genes hxtB-E. We evaluated ability of hxtB-E to functionally complement Saccharomyces cerevisiae EBY.VW4000 strain that is unable grow on glucose, fructose, mannose or galactose as single carbon source. In S. HxtB-E were targeted plasma membrane. The expression HxtB, HxtC and HxtE was able restore growth galactose, indicating these transporters...
Aspergillus fumigatus is an opportunistic pathogenic fungus able to infect immunocompromised patients, eventually causing disseminated infections that are difficult control and lead high mortality rates. It important understand how the signaling pathways regulate these factors involved in virulence orchestrated. Protein phosphatases central numerous signal transduction pathways. Here, we characterize A. protein phosphatase 2A SitA, Saccharomyces cerevisiae Sit4p homologue. The sitA gene not...
The serine-threonine kinase TOR, the Target of Rapamycin, is an important regulator nutrient, energy and stress signaling in eukaryotes. Sch9, a Ser/Thr AGC family (the cAMP-dependent PKA, cGMP- dependent protein G phospholipid-dependent C family), substrate TOR. Here, we characterized fungal opportunistic pathogen Aspergillus fumigatus Sch9 homologue (SchA). schA null mutant was sensitive to rapamycin, high concentrations calcium, hyperosmotic SchA involved iron metabolism. ΔschA showed...
Crude glycerol is the main byproduct of biodiesel industry. Although it can have different applications, its purification costly. Therefore, in this study a biotechnological route has been proposed for further utilization crude fermentative production lactic acid. This acid largely utilized food, pharmaceutical, textile, and chemical industries, making hydroxycarboxylic with highest market potential worldwide. Currently, industrial done mainly using sugar as substrate. Thus here, first time,...
Aspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections, such as invasive pulmonary aspergillosis in immunocompromised patients. The mitogen-activated protein kinase (MAPK) signaling pathways are essential for fungal adaptation to the host. Fungal cell survival, fungicide tolerance, and virulence highly dependent on organization, composition, function of wall. Upon wall stress, MAPKs phosphorylate multiple target proteins involved remodeling...
Invasive fungal infections (IFIs) are responsible for elevated rates of morbidity and mortality, causing around 1.5 million deaths annually worldwide. One the main causative agents IFIs is Candida albicans, non-albicans species have emerged as a spreading global public health concernment. Furthermore, COVID-19 has contributed to boost in incidence IFIs, such mucormycosis, which Rhizopus oryzae most prevalent agent. The effector host immune response against depends on activity T cells,...
Abstract The role of the fungal mitochondria goes far beyond energy metabolism. genomes 318 Aspergillus fumigatus clinical and environmental isolates from different geographic origins were analyzed aiming to study mitochondrial sequences populations sensitive resistant azoles. Our results show that A. mitogenomic are very conserved only variation in small intergenic regions one intronic sequence cox 3 gene. Furthermore, a genome-wide association analysis accessory genes revealed potential...
Aspergillus fumigatus is a major opportunistic pathogen and allergen of mammals. Nutrient sensing acquisition mechanisms, as well the capability to cope with different stressing conditions, are essential for A. virulence survival in mammalian host. This study characterized SebA transcription factor, which putative homologue factor encoded by Trichoderma atroviride seb1. The ΔsebA mutant demonstrated reduced growth presence paraquat, hydrogen peroxide, CaCl2, poor nutritional while viability...
Summary Nutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms taking up various nutrients. Despite its importance, only limited number nutrient receptors their corresponding ligands been identified in fungi. G ‐protein coupled ( GPCRs ) the largest family transmembrane receptors. The A spergillus nidulans genome encodes 16 putative , but few functionally characterised. Our previous study showed increased...
Aspergillus fumigatus is a fungal pathogen that causes several invasive and noninvasive diseases named aspergillosis. This disease generally regarded as multifactorial, considering pathogenicity determinants are present during the establishment of this illness. It necessary to obtain an increased knowledge how, which, A. signal transduction pathways engaged in regulation these processes. Protein phosphatases essential pathways. We identified 32 phosphatase catalytic subunit-encoding genes...
Metal restriction imposed by mammalian hosts during an infection is a common mechanism of defence to reduce or avoid the pathogen infection. Metals are essential for organism survival due its involvement in several biological processes. Aspergillus fumigatus causes invasive aspergillosis, disease that typically manifests immunocompromised patients. A. PpzA, catalytic subunit protein phosphatase Z (PPZ), has been recently identified as associated with iron assimilation. 2 high-affinity...