A5004571117- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Fungal Infections and Studies
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Microbial Natural Products and Biosynthesis
- Infectious Diseases and Mycology
- Plant Pathogens and Fungal Diseases
- Peptidase Inhibition and Analysis
- Plant-Microbe Interactions and Immunity
- Bee Products Chemical Analysis
- Nail Diseases and Treatments
- Plant Pathogenic Bacteria Studies
- Antimicrobial Peptides and Activities
- Probiotics and Fermented Foods
- Insect and Pesticide Research
- Plant Disease Resistance and Genetics
- Protein Tyrosine Phosphatases
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Bacterial biofilms and quorum sensing
- Plant Virus Research Studies
- Alkaline Phosphatase Research Studies
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
Universidade de São Paulo
2016-2025
Universidade de Ribeirão Preto
2014-2024
Universidade Federal do Pará
2022
Instituto de Ciências Farmacêuticas
2021
Here, we investigated which stress responses were influenced by the MpkC and SakA mitogen-activated protein kinases of high-osmolarity glycerol (HOG) pathway in fungal pathogen Aspergillus fumigatus. The ΔsakA double ΔmpkC mutants more sensitive to osmotic oxidative stresses, cell wall damaging agents. Both MpkC::GFP SakA::GFP translocated nucleus upon damage, with showing a quicker response. phosphorylation state MpkA was determined post exposure high concentrations congo red Sorbitol. In...
Abstract Fungal infections cause more than 1.5 million deaths a year. Due to emerging antifungal drug resistance, novel strategies are urgently needed combat life-threatening fungal diseases. Here, we identify the host defense peptide mimetic, brilacidin (BRI) as synergizer with caspofungin (CAS) against CAS-sensitive and CAS-resistant isolates of Aspergillus fumigatus, Candida albicans , C. auris CAS-intrinsically resistant Cryptococcus neoformans . BRI also potentiates azoles A. fumigatus...
Abstract Aspergillus fumigatus is a saprophytic fungus that can cause variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production important for its virulence and must be tightly regulated to avoid excess toxicity the fungus. GT self-protection by GliT oxidoreductase GtmA methyltransferase activities related subcellular localization these enzymes how sequestered from cytoplasm increased cell damage. Here, we show GliT:GFP GtmA:GFP are localized in vacuoles during...
Abstract The Cell Wall Integrity (CWI) pathway is the primary signaling cascade that controls de novo synthesis of fungal cell wall, and in Saccharomyces cerevisiae this event highly dependent on RLM1 transcription factor. Here, we investigated function RlmA pathogen Aspergillus fumigatus. We show ΔrlmA strain exhibits an altered wall organization addition to defects related vegetative growth tolerance wall-perturbing agents. A genetic analysis indicated rlmA positioned downstream pkcA mpkA...
Aspergillus fumigatus is an opportunistic fungal pathogen that causes invasive aspergillosis (IA), a life-threatening disease in immunocompromised humans. The echinocandin caspofungin, adopted as second-line therapy combating IA, β-1,3-glucan synthase inhibitor, which, when used high concentrations, reverts the anticipated A. growth inhibition, phenomenon called "caspofungin paradoxical effect" (CPE). CPE has been widely associated with increased chitin content cell wall due to compensatory...
Acquisition and subsequent metabolism of different carbon nitrogen sources have been shown to play an important role in virulence attributes the fungal pathogen Aspergillus fumigatus, such as secretion host tissue-damaging proteases cell wall integrity. We examined relationship between metabolic processes catabolite repression (CCR), (NCR) a variety A. fumigatus clinical isolates. A considerable amount heterogeneity with respect degree CCR NCR was observed positive correlation neutropenic...
The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of disease 2019 (COVID-19), has already killed millions people. COVID-19 patient outcome can be further complicated secondary infections, such as COVID-19-associated pulmonary aspergillosis (CAPA).
Aspergillus fumigatus is an opportunistic human pathogen, which causes the life-threatening disease, invasive pulmonary aspergillosis. In fungi, cell wall homeostasis controlled by conserved Cell Wall Integrity (CWI) pathway. A. this signaling cascade partially characterized, but mechanisms it activated are not fully elucidated. study we investigated role of protein kinase C (PkcA) in cascade. Our results suggest that pkcA essential gene and response to stress. Subsequently, constructed...
Invasive aspergillosis is predominantly caused by Aspergillus fumigatus, and adaptations to stresses experienced within the human host are a prerequisite for survival virulence strategies of pathogen. The central signal transduction pathway operating during hyperosmotic stress high osmolarity glycerol mitogen-activated protein kinase cascade. A. fumigatus MpkC SakA, orthologues Saccharomyces cerevisiae Hog1p, constitute primary regulator response. We compared wild-type transcriptional...
Aspergillus fumigatus is a fungal pathogen that capable of adapting to different host niches and avoid defenses. An enhanced understanding how, which, A. signal transduction pathways are engaged in the regulation these processes essential for development improved disease control strategies. Protein phosphatases central numerous pathways. To comprehend functions protein fumigatus, 32 phosphatase catalytic subunit encoding genes were identified. We have recognized PtcB as one involved high...
Standing among the front defense strategies against pathogens, host phagocytic cells release various oxidants. Therefore, pathogens have to cope with stressful conditions at site of infection. Peroxiredoxins (Prx) are highly reactive and abundant peroxidases that can support virulence persistence in distinct hosts. Here, we revealed opportunistic human pathogen A. fumigatus presents three 1-Cys Prx (Prx6 subfamily), which is unprecedented. We showed PrxB PrxC were mitochondria, while Prx1...
Aspergillus fumigatus is an opportunistic fungal pathogen that secretes array of immune-modulatory molecules, including secondary metabolites (SMs), which contribute to enhancing fitness and growth within the mammalian host. Gliotoxin (GT) a SM interferes with function recruitment innate immune cells, are essential for eliminating A. during invasive infections. We identified C6 Zn cluster-type transcription factor (TF), subsequently named RglT, important oxidative stress resistance, GT...
Aspergillus fumigatus causes a range of human and animal diseases collectively known as aspergillosis. A . possesses expresses genetic determinants virulence, which facilitate colonisation disease progression, including the secretion mycotoxins. Gliotoxin (GT) is best studied mycotoxin with wide toxic effects that impair immune cell function. GT also highly to this fungus has evolved self-protection mechanisms include (i) efflux pump GliA, (ii) neutralising enzyme GliT, (iii) negative...
ABSTRACT The human pathogenic fungus Aspergillus fumigatus establishes dual biofilm interactions in the lungs with bacterium Pseudomonas aeruginosa . Screening of 21 A null mutants revealed seven (two G protein-coupled receptors, three mitogen-activated protein kinase a Gα protein, and one histidine receptor) reduced formation, specifically presence P. Transcriptional profiling metabolomics analysis secondary metabolites produced by these mutants, Δ gpaB ( encodes protein), showed GpaB...
Non-canonical conjugation of ATG8 proteins, including LC3, to single membranes implicates the autophagy machinery in cell functions unrelated metabolic stress. One such pathway is LC3-associated phagocytosis (LAP), which aids phagosome maturation and subsequent signaling upon cargo uptake mediated by certain innate immunity-associated receptors. Here, we show that a specific isoform RAB5 GTPases, molecular switches controlling early endosome traffic, necessary for LAP. We demonstrate RAB5c...
ABSTRACT Propolis, a natural product of plant resins, is used by the bees to seal holes in their honeycombs and protect hive entrance. However, propolis has also been folk medicine for centuries. Here, we apply power Saccharomyces cerevisiae as model organism studies genetics, cell biology, genomics determine how affects fungi at cellular level. Propolis able induce an apoptosis death response. increased exposure provides corresponding increase necrosis We showed that cytochrome c but not...
Candida albicans is the most common fungal pathogen of humans, forming both commensal and opportunistic pathogenic interactions, causing a variety skin soft tissue infections in healthy people. In immunocompromised patients C. can result invasive, systemic that are associated with high incidence mortality. Propolis complex mixture several resinous substances which collected from plants by bees. Here, we demonstrated fungicidal activity propolis against all three morphogenetic types...
Aspergillus fumigatus is a major opportunistic pathogen and allergen of mammals. Calcium homeostasis signaling essential for numerous biological processes also influences A. pathogenicity. The presented study characterized the function homologues three Saccharomyces cerevisiae calcium channels, voltage-gated Cch1, stretch-activated Mid1 vacuolar Yvc1. channels cchA, midA yvcA were regulated at transcriptional level by increased levels. YvcA::GFP fusion protein localized to vacuoles. Both...
Aspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling essential for A. pathogenicity regulated by the CrzA transcription factor. We used ChIP-seq (Chromatin Immunoprecipitation DNA sequencing) to explore gene targets in fumigatus. In total, 165 potential binding peaks including 102 directly genes were identified, resulting prediction A[GT][CG]CA[AC][AG] CrzA-binding motif. The putatively exhibited a diverse array functions. phkB (Afu3g12530) histidine...
Aspergillus fumigatus is an opportunistic pathogenic fungus able to infect immunocompromised patients, eventually causing disseminated infections that are difficult control and lead high mortality rates. It important understand how the signaling pathways regulate these factors involved in virulence orchestrated. Protein phosphatases central numerous signal transduction pathways. Here, we characterize A. protein phosphatase 2A SitA, Saccharomyces cerevisiae Sit4p homologue. The sitA gene not...
Aspergillus fumigatus causes invasive aspergillosis, the most common life-threatening fungal disease of immuno-compromised humans. The treatment disseminated infections with antifungal drugs, including echinocandin cell wall biosynthesis inhibitors, is increasingly challenging due to rise drug-resistant pathogens. calcium responsive calcineurin-CrzA pathway influences morphology, composition, virulence, and resistance. A screen 395 A. transcription factor mutants identified nine factors...
The serine-threonine kinase TOR, the Target of Rapamycin, is an important regulator nutrient, energy and stress signaling in eukaryotes. Sch9, a Ser/Thr AGC family (the cAMP-dependent PKA, cGMP- dependent protein G phospholipid-dependent C family), substrate TOR. Here, we characterized fungal opportunistic pathogen Aspergillus fumigatus Sch9 homologue (SchA). schA null mutant was sensitive to rapamycin, high concentrations calcium, hyperosmotic SchA involved iron metabolism. ΔschA showed...
Aspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections, such as invasive pulmonary aspergillosis in immunocompromised patients. The mitogen-activated protein kinase (MAPK) signaling pathways are essential for fungal adaptation to the host. Fungal cell survival, fungicide tolerance, and virulence highly dependent on organization, composition, function of wall. Upon wall stress, MAPKs phosphorylate multiple target proteins involved remodeling...
Aspergillus fumigatus , one of the most important human-pathogenic fungal species, is able to cause aspergillosis, a heterogeneous group diseases that presents wide range clinical manifestations. Invasive pulmonary aspergillosis serious pathology in terms patient outcome and treatment, with high mortality rate ranging from 50% 95% primarily affecting immunocompromised patients. Azoles have been used for many years as main antifungal agents treat prevent invasive aspergillosis. However, there...