A5012946900- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Mycotoxins in Agriculture and Food
- RNA Research and Splicing
- Fungal Biology and Applications
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Geological formations and processes
- Geological and Geophysical Studies
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Enterobacteriaceae and Cronobacter Research
- Enzyme Production and Characterization
- DNA Repair Mechanisms
- Landslides and related hazards
- Geological Modeling and Analysis
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
Trinity College Dublin
2011-2024
University of Edinburgh
1999-2014
Cancer Research UK Scotland Institute
2010
University of New Mexico
2004-2008
Heriot-Watt University
1995
We employ a numerical surface processes model to study the controls on postbreakup landscape development and denudational history of southeast African margin. Apatite fission track data, presented in companion paper, suggest that Drakensberg Escarpment formed by rapid river incision seaward preexisting drainage divide, located close its present position, subsequently retreated at rates only ∼100 m m.y. −1 . Numerical modeling results support such scenario show prebreakup topography margin...
Abstract Chromatin complexes control a vast number of epigenetic developmental processes. Filamentous fungi present an important clade microbes with poor understanding underlying mechanisms. Here, we describe chromatin binding complex in the fungus Aspergillus nidulans composing H3K4 histone demethylase KdmB, cohesin acetyltransferase (EcoA), deacetylase (RpdA) and reader/E3 ligase protein (SntB). In vitro vivo evidence demonstrate that this KERS is assembled from EcoA-KdmB SntB-RpdA...
Abstract Aspergillus fumigatus is a saprophytic fungus that can cause variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production important for its virulence and must be tightly regulated to avoid excess toxicity the fungus. GT self-protection by GliT oxidoreductase GtmA methyltransferase activities related subcellular localization these enzymes how sequestered from cytoplasm increased cell damage. Here, we show GliT:GFP GtmA:GFP are localized in vacuoles during...
Cosmogenic 36 C1 concentrations in basalt samples from the Drakensberg escarpment on SE African passive margin give quantitative estimates of denudation and scarp retreat rates. Over 10 4 –10 6 year timespan addressed by these data, calculated rate has been 50–95 m Ma -1 average summit . The is an order magnitude less than previously suggested. lowering sufficient to prevent long-term intact survival erosion cycle surfaces formed Mesozoic that were inferred for this region.
The filamentous fungus Aspergillus flavus is a plant and human pathogen predominantly found in the soil as spores or sclerotia capable of producing various secondary metabolites (SM) such carcinogenic mycotoxin aflatoxin. Recently, we have discovered novel nuclear chromatin binding complex (KERS) that contains JARID1-type histone demethylase KdmB, putative cohesion acetyl transferase EcoA, class I type deacetylase RpdA PHD ring finger reader protein SntB model nidulans. Here, show presence...
We demonstrate that the yeast flocculation gene, FLO1, is representative of a distinct subset subtelomeric genes are robustly repressed by Cyc8-Tup1 complex. have examined localisation, histone acetylation and long-range chromatin remodelling within extensive FLO1 upstream region. show localised in DNase I hypersensitive site an ordered array strongly positioned nucleosomes around -700 base pairs transcription start site. In cyc8 deletion mutant strains, Tup1p localisation absent, with...
The Saccharomyces cerevisiae FLO1 gene encodes a cell wall protein that imparts cell-cell adhesion. transcription is regulated via the antagonistic activities of Tup1-Cyc8 co-repressor and Swi-Snf co-activator complexes. represses through organization strongly positioned, hypoacetylated nucleosomes across promoters. catalyzes remodeling these in mechanism involving histone acetylation poorly understood. Here, we show de-repression accompanied by recruitment, promoter eviction Sas3...
The TLO genes are a family of telomere-associated ORFs in the fungal pathogens Candida albicans and C. dubliniensis that encode subunit Mediator complex with homology to Med2. more virulent pathogen has 15 copies gene whereas less pathogenic species only two (CdTLO1 CdTLO2). In this study we used as model investigate role regulating virulence also determine whether paralogs have evolved regulate distinct functions. A tlo1Δ/tlo2Δ mutant is unable form true hyphae, longer doubling times...
Quiescent cells have a low level of gene activity compared to growing cells. Using yeast model for cellular quiescence, we defined the genome-wide profiles three species histone methylation associated with active transcription between and quiescent cells, correlated these presence RNA polymerase II transcripts. retained methylations normally transcriptionally chromatin had many transcripts in common also contained significant levels II, but only canonical initiating elongating forms...
For eukaryotes like fungi to regulate biological responses environmental stimuli, various signalling cascades are utilized, the highly conserved mitogen-activated protein kinase (MAPK) pathways. In model fungus Aspergillus nidulans, a MAPK pathway known as pheromone module regulates development and production of secondary metabolites (SMs). This consists five proteins, three kinases SteC, MkkB MpkB, adaptor SteD scaffold HamE. this study, homologs these proteins have been identified in plant...
The Tup1-Cyc8 complex in Saccharomyces cerevisiae was one of the first global co-repressors gene transcription discovered. However, despite years study, a full understanding contribution Tup1p and Cyc8p to function is lacking. We examined TUP1 CYC8 single double deletion mutants show that represses more genes than , there are subject (i) unique repression by or (ii) redundant (iii) at which de-repression cyc8 mutant dependent upon vice-versa. also reveal can make distinct contributions...
The packaging of DNA into chromatin regulates transcription from initiation through 3' end processing. One aspect in which plays a poorly understood role is the co-transcriptional splicing pre-mRNA.Here we provide evidence that H2B monoubiquitylation (H2BK123ub1) marks introns Saccharomyces cerevisiae. A genome-wide map H2BK123ub1 this organism reveals modification enriched coding regions and its levels peak at transcribed two characteristic subgroups genes. First, long genes are more likely...
The high-osmolarity glycerol (HOG) response pathway is a multifunctional signal transduction that specifically transmits ambient osmotic signals. Saccharomyces cerevisiae Hog1p has two upstream signaling branches, the sensor histidine kinase Sln1p and receptor Sho1p. Sho1p branch includes other proteins, Msb2p mucin Opy2p. Aspergillus fumigatus leading cause of pulmonary fungal diseases. Here, we investigated roles played by A. SlnASln1p, ShoASho1p, MsbAMsb2p, OpyAOpy2p putative homologues...
In order for eukaryotes to efficiently detect and respond environmental stimuli, a myriad of protein signaling pathways are utilized. An example highly conserved in the mitogen-activated kinase (MAPK) pathways. fungi, MAPK have been shown regulate diverse array biological processes, such as asexual sexual development, stress responses production secondary metabolites (SMs). model fungus Aspergillus nidulans, pathway known pheromone module is utilized both development SM production. This...
The traditional model for chromatin remodelling during transcription has focused upon the of nucleosomes at gene promoters. However, in this study, we have determined that Tup1-Ssn6 and Swi-Snf activities extend far upstream SUC2 promoter into intergenic region Saccharomyces cerevisiae chromosome. We mapped nucleosomal array over a 7.5 kb encompassed but was devoid other transcriptionally active genes. Nucleosome positioning under conditions glucose repression derepression, snf2, ssn6 snf2...
A deletion of the spoIIAC gene Bacillus licheniformis was prepared in vitro by using splicing-by-overlap-extension technique. This introduced into B. on a temperature-sensitive plasmid, and following integration excision from chromosome, precisely located chromosomal prepared. The mutated bacterium totally asporogenous formed abortively disporic cells characterized asymmetric septa at poles cells. Qualitative plate tests revealed that synthesized normal levels DNase, polygalacturonate lyase,...
We report here the draft genome sequence of Acinetobacter junii MHI21018, isolated in 2009 from bovine colostrum. The is composed 3,267,995 bp, has a GC content 38.54%, and was assembled into 114 contigs (contig size, >500 bp) with an N50 value 72,566 bp.
The transcriptome from a Saccharomyces cerevisiae tup1 deletion mutant was one of the first comprehensive yeast transcriptomes published. Subsequent and cyc8 mutants firmly established Tup1-Cyc8 complex as predominantly acting repressor gene transcription. However, tup1/cyc8 or conditional would all have been influenced by striking flocculation phenotypes that these display. In this study, we separated impact in to reveal those genes (i) subject solely Cyc8p-dependent regulation, (ii)...