A5053724622- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Gut microbiota and health
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Pathogens and Fungal Diseases
- Parasitic Infections and Diagnostics
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Environmental DNA in Biodiversity Studies
- Ethics in Clinical Research
- Clostridium difficile and Clostridium perfringens research
- Diet, Metabolism, and Disease
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- interferon and immune responses
- Parasitic Diseases Research and Treatment
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Cystic Fibrosis Research Advances
- Complement system in diseases
- Wildlife Ecology and Conservation
- Biochemical Analysis and Sensing Techniques
The Ohio State University
2017-2025
University of Wisconsin–Madison
2023-2025
Ohio University
2022
Certara (United States)
2022
Florida State University
2021
Seattle University
2020
University of Nebraska Medical Center
2019
Brown University
2014-2018
Broward College
2017
College of Saint Benedict and Saint John's University
2016
Candida albicans is a commensal fungus of the human gastrointestinal tract and prevalent opportunistic pathogen. To examine diversity within this species, extensive genomic phenotypic analyses were performed on 21 clinical C. isolates. Genomic variation was evident in form polymorphisms, copy number variations, chromosomal inversions, subtelomeric hypervariation, loss heterozygosity (LOH), whole or partial chromosome aneuploidies. All strains diploid, although karyotypic changes present...
Candida albicans surface-attached biofilms such as those formed on intravenous catheters with direct access to the bloodstream often serve a nidus for continuous release of cells capable initiating new infectious foci. We previously reported that dispersed from biofilm are yeast originate top-most hyphal layers biofilm. Compared their planktonic counterparts, these dispersal displayed enhanced virulence-associated characteristics and drug resistance. However, little is known about molecular...
Abstract Background Non-caloric artificial sweeteners (NCAS) are widely used as a substitute for dietary sugars to control body weight or glycemia. Paradoxically, some interventional studies in humans and rodents have shown unfavorable changes glucose homeostasis response NCAS consumption. The causative mechanisms largely unknown, but adverse gut microbiota been proposed mediate these effects. These findings raised concerns about safety called into question their broad use, further...
The field of genomics has benefited greatly from its “openness” approach to data sharing. However, with the increasing volume sequence information being created and stored growing number international efforts, equity openness is under question. United Nations Convention Biodiversity aims develop adopt a standard policy on access benefit-sharing for across signatory parties. This standardization will have profound implications research, requiring new definition open redefinition not...
Butyrate is an abundant metabolite produced by gut microbiota. While butyrate a known histone deacetylase inhibitor that activates expression of many genes involved in immune system pathways, its effects on virus infections and the antiviral type I interferon (IFN) response have not been adequately investigated. We found increases cellular infection with viruses relevant to human animal health, including influenza virus, reovirus, HIV-1, metapneumovirus, vesicular stomatitis virus....
Candida albicans is an important opportunistic fungal pathogen capable of causing both mucosal and disseminated disease. Infections are often treated with fluconazole, a front-line antifungal drug that targets the biosynthesis ergosterol, major component cell membrane. Resistance to fluconazole can arise through variety mechanisms, including gain-of-function mutations, loss heterozygosity events aneuploidy. The clinical isolate P60002 was found be highly resistant azole-class drugs, yet...
Mutations introduce variation into the genome upon which selection can act. Defining nature of these changes is critical for determining species evolution, as well understanding genetic driving important cellular processes. The heterozygous diploid fungus Candida albicans both a frequent commensal organism and prevalent opportunistic pathogen. A prevailing theory that C. evolves primarily through gradual buildup mitotic mutations, pressing issue whether sexual or parasexual processes also...
Abstract Subtelomeric regions of the genome are notable for high rates sequence evolution and rapid gene turnover. Evidence subtelomeric has relied heavily on comparisons historical evolutionary patterns to infer trends frequencies these events. Here, we describe TLO family in Candida albicans during laboratory passaging over 4000 generations. C. is a commensal opportunistic pathogen humans encodes subunit Mediator complex that regulates transcription affects range virulence factors. We...
Meiosis is a conserved tenet of sexual reproduction in eukaryotes, yet this program seemingly absent from many extant species. In the human fungal pathogen Candida albicans, mating diploid cells generates tetraploid products that return to state via non-meiotic process depolyploidization known as concerted chromosome loss (CCL). Here, we report recombination rates are more than three orders magnitude higher during CCL normal mitotic growth. Furthermore, two 'meiosis-specific' factors play...
Candida albicans grows within a wide range of host niches, and this adaptability enhances its success as commensal pathogen. The telomere-associated TLO gene family underwent recent expansion from one or two copies in other CUG clade members to 14 expressed C. albicans. This correlates with increased virulence clinical prevalence relative those species. have conserved Med2 domain at the N terminus, suggesting role general transcription. C-terminal half is more divergent, distinguishing three...
The TLO genes are a family of telomere-associated ORFs in the fungal pathogens Candida albicans and C. dubliniensis that encode subunit Mediator complex with homology to Med2. more virulent pathogen has 15 copies gene whereas less pathogenic species only two (CdTLO1 CdTLO2). In this study we used as model investigate role regulating virulence also determine whether paralogs have evolved regulate distinct functions. A tlo1Δ/tlo2Δ mutant is unable form true hyphae, longer doubling times...
Infectious fungal species are often treated uniformly despite clear evidence of genotypic and phenotypic heterogeneity being widespread across strains. Identifying the genetic basis for this diversity is extremely challenging because tens or hundreds thousands variants that may distinguish two
ABSTRACT Sickle cell disease (SCD) is a chronic blood disorder that disrupts multiple organ systems and can lead to severe morbidity. Persistent acute symptoms caused by immune system dysregulation in individuals with SCD could contribute either directly or indirectly via dysbiosis of commensal microbes increased susceptibility infection. Here, we explored the nasal oral microbiomes children (cwSCD) uncover potential dysbiotic associations disorder. Microbiota collected from swabs 40 cwSCD...
Cell-to-cell gene expression noise is thought to be an important mechanism for generating phenotypic diversity. Furthermore, telomeric regions are major sites amplification, which drive genetic Here we found that individual subtelomeric TLO genes exhibit increased variation in transcript and protein levels at both the cell-to-cell level as well population-level. The variation, termed Telomere-Adjacent Gene Expression Noise (TAGEN) was largely intrinsic dependent upon genome position: reduced...
Phosphorus is essential in all cells’ structural, metabolic and regulatory functions. For fungal cells that import inorganic phosphate (Pi) up a steep concentration gradient, surface Pi transporters are critical capacitators of growth. Fungi must deploy enable optimal uptake pH ranges prevalent their environments. Single, triple quadruple mutants were used to characterize the four we identified for human pathogen Candida albicans , which adapt alkaline conditions during invasion host...
The human fungal pathobiont Candida albicans displays extensive genomic plasticity, including large-scale chromosomal changes such as aneuploidy. Chromosome trisomy appears frequently in natural and laboratory strains of C. . Trisomy specific chromosomes has been linked to large phenotypic effects, increased murine gut colonization by trisomic for chromosome 7 (Chr7). However, studies whole-chromosome aneuploidy are generally limited the SC5314 genome reference strain, making it unclear...