A5073207690- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Toxoplasma gondii Research Studies
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mycotoxins in Agriculture and Food
- Herpesvirus Infections and Treatments
- Antimicrobial Peptides and Activities
- Nail Diseases and Treatments
- Microbial Natural Products and Biosynthesis
- Cytomegalovirus and herpesvirus research
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Synthesis and Biological Evaluation
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Plant Pathogens and Fungal Diseases
- Gut microbiota and health
- Hormonal and reproductive studies
- Autophagy in Disease and Therapy
- Galectins and Cancer Biology
- Sirtuins and Resveratrol in Medicine
- Cancer-related gene regulation
- Eosinophilic Esophagitis
- X-ray Diffraction in Crystallography
Universidade de São Paulo
2012-2025
JP Group (Brazil)
2025
Universidade de Ribeirão Preto
2013-2024
Hospital Universitário da Universidade de São Paulo
2023
Fundação Oswaldo Cruz
2021
Abstract Fungal infections cause more than 1.5 million deaths a year. Due to emerging antifungal drug resistance, novel strategies are urgently needed combat life-threatening fungal diseases. Here, we identify the host defense peptide mimetic, brilacidin (BRI) as synergizer with caspofungin (CAS) against CAS-sensitive and CAS-resistant isolates of Aspergillus fumigatus, Candida albicans , C. auris CAS-intrinsically resistant Cryptococcus neoformans . BRI also potentiates azoles A. fumigatus...
Abstract Aspergillus fumigatus is a saprophytic fungus that can cause variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production important for its virulence and must be tightly regulated to avoid excess toxicity the fungus. GT self-protection by GliT oxidoreductase GtmA methyltransferase activities related subcellular localization these enzymes how sequestered from cytoplasm increased cell damage. Here, we show GliT:GFP GtmA:GFP are localized in vacuoles during...
This study aimed to investigate the immunological mechanisms involved in gender distinct incidence of paracoccidioidomycosis (pcm), an endemic systemic mycosis Latin America, which is at least 10 times more frequent men than women. Then, we compared immune response male and female mice Paracoccidioides brasiliensis infection, as well influence differences exerted by paracoccin, a P. component with carbohydrate recognition property. High production Th1 cytokines T-bet expression have been...
Toxoplasmosis, a zoonotic disease caused by Toxoplasma gondii, is an important public health problem and veterinary concern. Although there no vaccine for human toxoplasmosis, many attempts have been made to develop one. Promising candidates utilize proteins, or their genes, from microneme organelle of T. gondii that are involved in the initial stages host cell invasion parasite. In present study, we used different recombinant proteins (TgMIC1, TgMIC4, TgMIC6) combinations these (TgMIC1-4...
Infection of host cells by Toxoplasma gondii is an active process, which regulated secretion microneme (MICs) and rhoptry proteins (ROPs RONs) from specialized organelles in the apical pole parasite. MIC1, MIC4 MIC6 assemble into adhesin complex secreted on parasite surface that functions to promote infection competency. MIC1 are known bind terminal sialic acid residues galactose residues, respectively induce IL-12 production splenocytes. Here we show rMIC1- rMIC4-stimulated dendritic...
ABSTRACT Fungal pathogens pose a serious threat to human health, with Candida and Aspergillus spp. representing some of the most significant opportunistic invaders. fumigatus causes aspergillosis, one prevalent fungal diseases humans. There is limited number drugs available combat these infections, antifungal drug resistance on rise. In this manuscript, we show 4-[4-(4-Piperidinyl) phenyl]-7-[4-(-(trifluoromethyl) phenyl]-2-naphthalenecarboxylic acid (PPTN), highly specific antagonist P2Y14...
ABSTRACT The human pathogenic fungus Aspergillus fumigatus establishes dual biofilm interactions in the lungs with bacterium Pseudomonas aeruginosa . Screening of 21 A null mutants revealed seven (two G protein-coupled receptors, three mitogen-activated protein kinase a Gα protein, and one histidine receptor) reduced formation, specifically presence P. Transcriptional profiling metabolomics analysis secondary metabolites produced by these mutants, Δ gpaB ( encodes protein), showed GpaB...
ABSTRACT Non-canonical conjugation of ATG8 proteins, including LC3, to single membranes implicates the autophagy machinery in cell functions unrelated metabolic stress. One such pathway is LC3-associated phagocytosis (LAP), which aids phagosome maturation and subsequent signaling upon cargo uptake mediated by certain innate immunity-associated receptors. Here, we show that a specific isoform RAB5 GTPases, molecular switches controlling early endosome traffic, necessary for LAP. We...
Background Leishmaniasis is a complex disease in which clinical outcome depends on factors such as parasite species, host genetics and immunity vector species. In Brazil, Leishmania (Viannia) braziliensis major etiological agent of cutaneous (CL) mucosal leishmaniasis (MCL), disfiguring form the disease, occurs ~10% L. braziliensis-infected patients. Thus, isolates from patients with CL MCL may be relevant source information to uncover contributing pathogenesis. this study, we investigated...
Abstract Aspergillus fumigatus represents a public health problem due to the high mortality rate in immunosuppressed patients and emergence of antifungal-resistant isolates. Protein acetylation is crucial post-translational modification that controls gene expression biological processes. The strategic manipulation enzymes involved protein has emerged as promising therapeutic approach for addressing fungal infections. Sirtuins, NAD + -dependent lysine deacetylases, regulate eukaryotes....
ABSTRACT Cryptococcus neoformans causes cryptococcosis, one of the most prevalent fungal diseases, generally characterized by meningitis. There is a limited and not very effective number drugs available to combat this disease. In manuscript, we show host defense peptide mimetic brilacidin (BRI) as promising antifungal drug against C. . BRI can affect organization cell membrane, increasing permeability. We also investigated effects model system Saccharomyces cerevisiae analyzing libraries...
Dysbiosis and disturbances in gut homeostasis may result dysregulated responses, which are common inflammatory bowel diseases (IBD). These conditions be refractory to the usual treatments novel therapies still necessary reach a more successful regulation of intestinal immunity. The hormone melatonin (MLT) has been raised as therapeutic alternative because its known interactions with immune responses microbiota. Hence, we evaluated effects MLT experimental colitis that evolves dysbiosis,...
Toxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential various biological processes, some pathogens rely on chitinases for successful parasitization. Here, we purified characterized a chitinase from T. gondii. The enzyme, provisionally named Tg_chitinase, has molecular mass of 13.7 kDa exhibits Km 0.34 mM Vmax 2.64. optimal environmental...
ABSTRACT Sporotrichosis, the cutaneous mycosis most commonly reported in Latin America, is caused by Sporothrix clinical clade species, including brasiliensis and schenckii sensu stricto . Due to its zoonotic transmission Brazil, S. represents a significant health threat humans domestic animals. Itraconazole, terbinafine, amphotericin B are used antifungals for treating sporotrichosis. However, many strains of have shown resistance these agents, highlighting importance finding new...
The microneme organelles of Toxoplasma gondii tachyzoites release protein complexes (MICs), including one composed the transmembrane MIC6 plus MIC1 and MIC4. In this complex, carbohydrate recognition domains MIC4 are exposed interact with terminal sialic acid galactose residues, respectively, host cell glycans. Recently, we demonstrated that binding to N-glycans Toll-like receptor (TLR) 2 TLR4 on phagocytes triggers activation pro-inflammatory cytokine production. Herein, investigated...
The leishmaniasis is a spectral disease caused by the protozoan L eishmania spp., which threatens millions of people worldwide. Current treatments exhibit high toxicity, and there no vaccine available. need for new lead compounds with leishmanicidal activity urgent. Considering that many contain quinoidal scaffold thiazole heterocyclic ring found in number antimicrobial drugs, we proposed hybridization approach to generate diverse set semi‐synthetic heterocycles antileishmanial activity. We...