A5065428778- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Infectious Diseases and Mycology
- Pediatric health and respiratory diseases
- Antimicrobial Peptides and Activities
- Inflammasome and immune disorders
- Nail Diseases and Treatments
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Mycotoxins in Agriculture and Food
- Tryptophan and brain disorders
- Exercise and Physiological Responses
- Immune Cell Function and Interaction
- Whipple's Disease and Interleukins
- Immune cells in cancer
- Inflammation biomarkers and pathways
- Muscle Physiology and Disorders
- Immunotherapy and Immune Responses
- Toxoplasma gondii Research Studies
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Parasitic Infections and Diagnostics
- SARS-CoV-2 and COVID-19 Research
Universidade Federal de São Paulo
2018-2024
Federal Institute of São Paulo
2021-2024
Universidade de São Paulo
2011-2021
University of Massachusetts Chan Medical School
2015
To study the role of TLR2 in a experimental model chronic pulmonary infection, TLR2-deficient and wild-type mice were intratracheally infected with Paracoccidioides brasiliensis, primary fungal pathogen. Compared control, TLR2(-/-) developed less severe infection decreased NO synthesis. Equivalent results detected vitro-infected macrophages. Unexpectedly, despite differences loads both mouse strains showed equivalent survival times inflammatory reactions. Studies on lung-infiltrating...
Plasmacytoid dendritic cells (pDCs) were initially considered as critical for innate immunity to viruses. However, our group has shown that pDCs bind and inhibit the growth of Aspergillus fumigatus hyphae depletion renders mice hypersusceptible experimental aspergillosis. In this study, we examined pDC receptors contributing hyphal recognition downstream events in stimulated by A. hyphae. Our data show Dectin-2, but not Dectin-1, participates recognition, TNF-α IFN-α release, antifungal...
In infectious diseases, the enzyme indoleamine 2,3 dioxygenase-1 (IDO1) that catalyzes tryptophan (Trp) degradation along kynurenines (Kyn) pathway has two main functions, control of pathogen growth by reducing available Trp and immune regulation mediated Kyn-mediated expansion regulatory T (Treg) cells via aryl hydrocarbon receptor (AhR). pulmonary paracoccidioidomycosis (PCM) caused dimorphic fungus Paracoccidioides brasiliensis, IDO1 was shown to disease severity both resistant...
ABSTRACT Toll-like receptors (TLRs) present in innate immune cells recognize pathogen molecular patterns and influence immunity to control the host-parasite interaction. The objective of this study was characterize involvement TLR4 adaptive Para co c cidioides brasiliensis , most important primary fungal Latin America. We compared responses C3H/HeJ mice, which are naturally defective signaling, with those C3H/HePas express functional receptors, after vitro vivo infection P. . Unexpectedly,...
Dectin-1, the innate immune receptor that recognizes β-glucan, plays an important role in immunity against fungal pathogens. Paracoccidioides brasiliensis, etiological agent of paracoccidioidomycosis, has a sugar-rich cell wall mainly composed mannans and glucans. This fact motivated us to use dectin-1–sufficient –deficient mice investigate β-glucan recognition pulmonary paracoccidioidomycosis. Initially, we verified P. brasiliensis infection reinforced tendency dectin-1–deficient...
The concomitant use of diverse pattern recognition receptors (PRRs) by innate immune cells can result in synergistic or inhibitory activities that profoundly influence anti-microbial immunity. Dectin-1 and the mannose receptor (MR) are C-type lectin (CLRs) previously reported to cooperate with toll-like (TLRs) signaling initial inflammatory response induction adaptive Th17 Tc17 immunity mediated CD4(+) CD8(+) T cells, respectively. protective against paracoccidioidomycosis, most prevalent...
Regulatory T (Treg) cells are fundamental in the control of immunity and excessive tissue pathology. In paracoccidioidomycosis, an endemic mycosis Latin America, immunoregulatory mechanisms that progressive regressive forms this infection poorly known. Due to its modulatory activity on Treg cells, we investigated effects anti-CD25 treatment over course pulmonary resistant (A/J) susceptible (B10.A) mice infected with Paracoccidioides brasiliensis. We verified A/J developed higher numbers more...
ABSTRACT The mechanisms that govern the initial interaction between Paracoccidioides brasiliensis , a primary dimorphic fungal pathogen, and cells of innate immunity need to be clarified. Our previous studies showed Toll-like receptor 2 (TLR2) TLR4 regulate with macrophages pattern adaptive further develops. aim present investigation was assess role MyD88, an adaptor molecule used by TLRs activate genes inflammatory response in pulmonary paracoccidioidomycosis. Studies were performed normal...
The NOD-Like Receptor P3 (NLRP3) inflammasome is an intracellular multimeric complex that triggers the activation of inflammatory caspases and maturation IL-1β IL-18, important cytokines in innate immune response against pathogens. functional NLRP3 consists NLRP3, adaptor protein ASC caspase-1. Various molecular mechanisms are associated with activation, including presence extracellular ATP, which recognized by cell surface P2X7 receptor (P2X7R). Several pattern recognition receptors (PRRs)...
Aspergillus nidulans is an opportunistic fungal pathogen in patients with immunodeficiency, and virulence of A. isolates has mainly been studied the context chronic granulomatous disease (CGD), characterization clinical obtained from non-CGD remaining elusive. This study therefore carried out a detailed biological two (CIs), patient breast carcinoma pneumonia cystic fibrosis that underwent lung transplantation, compared them to reference, nonclinical FGSC A4 strain. Both CIs presented...
Aspergillus fumigatus is an opportunistic fungal pathogen that secretes array of immune-modulatory molecules, including secondary metabolites (SMs), which contribute to enhancing fitness and growth within the mammalian host. Gliotoxin (GT) a SM interferes with function recruitment innate immune cells, are essential for eliminating A. during invasive infections. We identified C6 Zn cluster-type transcription factor (TF), subsequently named RglT, important oxidative stress resistance, GT...
In addition to alpha1,3 glucan, mannan and mannan-linked proteins are expressed in the outer layer of Paracoccidioides brasiliensis yeasts. The recognition mannosyl residues by multiple pathogen receptors, such as mannose receptor (MR), complement 3 (CR3) toll-like 4 (TLR4) on macrophage membranes can influence activation mechanisms innate immunity against fungal pathogens. aim this study was clarify role these receptors interaction between P. macrophages from resistant (A/J) susceptible...
Dectin-1 is a pattern recognition receptor (PRR) that recognizes β-glucans and plays major role in the immunity against fungal pathogens. Paracoccidioides brasiliensis, causative agent of paracoccidioidomycosis, has sugar-rich cell wall mainly composed mannans glucans. To investigate dectin-1 innate resistant (A/J) susceptible (B10.A) mice to P. brasiliensis infection, we evaluated curdlan (a agonist) laminarin antagonist) activation macrophages from both mouse strains. We verified...
Plasmacytoid dendritic cells (pDCs), considered critical for immunity against viruses, were recently associated with defense mechanisms fungal infections. However, the immunomodulatory function of pDCs in pulmonary paracoccidiodomycosis (PCM), an endemic infection Latin America, has been poorly defined. Here, we investigated role pathogenesis PCM caused by 129Sv mice 1 x 106 P. brasiliensis-yeasts. In vitro experiments showed that brasiliensis induces maturation and elevated synthesis TNF-α...
The underlying mechanism by which MyD88 regulates the development of obesity, metainflammation, and insulin resistance (IR) remains unknown. Global deletion in high-fat diet (HFD)-fed mice resulted increased weight gain, impaired glucose homeostasis, elevated Dectin-1 expression adipose tissue (AT), proinflammatory CD11c+ AT macrophages (ATMs). KO were protected from diet-induced obesity (DIO) IR had reduced macrophages. antagonist improved homeostasis decreased chow- HFD-fed mice. agonist...
Aspergillus fumigatus causes invasive aspergillosis, the most common life-threatening fungal disease of immuno-compromised humans. The treatment disseminated infections with antifungal drugs, including echinocandin cell wall biosynthesis inhibitors, is increasingly challenging due to rise drug-resistant pathogens. calcium responsive calcineurin-CrzA pathway influences morphology, composition, virulence, and resistance. A screen 395 A. transcription factor mutants identified nine factors...
In human paracoccidioidomycosis (PCM), a primary fungal infection typically diagnosed when the disease is already established, regulatory T cells (Treg) are associated with severity. Experimental studies in pulmonary PCM confirmed detrimental role of these cells, but most studies, Tregs were depleted prior to or early during infection. These facts led us study effects Treg cell depletion using model ongoing PCM. Therefore, was achieved by treatment transgenic C57BL/6
Likely as in other viral respiratory diseases, SARS-CoV-2 elicit a local immune response, which includes production and releasing of both cytokines secretory immunoglobulin (SIgA). Therefore, this study, we investigated the levels specific-SIgA for airways mucosa 37 patients who were suspected COVID-19. According to RT-PCR results, separated into three groups: negative COVID-19 viruses (NEGS, n = 5); but positive presence (OTHERS, (COVID-19, 27). Higher SARS-CoV-2, IFN-β, IFN-γ found group...
The development of engineered nanomaterials has been considered a promising strategy to control oral infections. In this study, silver-embedded carbon nitrides (Ag@g-CN) were synthesized and tested against Candida albicans, investigating their antifungal action biocompatibility in animal cells. Ag@g-CN was by simple one-pot thermal polymerization technique characterized various analytical techniques. X-ray diffraction (XRD) analysis revealed slight alterations the crystal structure g-CN upon...
The protective adaptive immune response in paracoccidioidomycosis, a mycosis endemic among humans, is mediated by T cell immunity, whereas impaired responses are associated with severe, progressive disease. early host to Paracoccidioides brasiliensis infection not known since the disease diagnosed at later phases of infection. Our laboratory established murine model where susceptible mice reproduce severe disease, while resistant develop mild This work aimed characterize influence dendritic...
Previous studies on paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in Latin America, revealed that host immunity is tightly regulated by several suppressive mechanisms mediated tolerogenic plasmacytoid dendritic cells, enzyme 2,3 indoleamine dioxygenase (IDO-1), and regulatory T-cells (Tregs). IDO-1 orchestrates local immunosuppressive effects through recruitment activation of myeloid-derived suppressor cells (MDSCs), a heterogeneous population myeloid possessing potent...
Background Cellular immunity is the main defense mechanism in paracoccidioidomycosis (PCM), most important systemic mycosis Latin America. Th1 and IFN-γ activated macrophages are fundamental to immunoprotection that antagonized by IL-10, an anti-inflammatory cytokine. Both human experimental PCM, several evidences indicate suppressive effect of IL-10 causes detrimental effects infected hosts. Because direct studies have not been performed, this study was aimed characterize function pulmonary...
Plasmacytoid dendritic cells (pDCs), which have been extensively studied in the context of immune response to viruses, recently implicated host defense mechanisms against fungal infections. Nevertheless, involment human pDCs during paracoccidiodomycosis (PCM), a infection endemic Latin America, has scarcerly studied. However, were found cutaneous lesions PCM patients, and pulmonary model murine these shown control disease severity. These findings led us investigate role innate phase PCM....