A5019614963- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antifungal resistance and susceptibility
- Immune Response and Inflammation
- Bacterial biofilms and quorum sensing
- Fungal Infections and Studies
- Immune cells in cancer
- Aquaculture disease management and microbiota
- Vitamin C and Antioxidants Research
- Legionella and Acanthamoeba research
- Ocular Infections and Treatments
- Infectious Diseases and Mycology
- Protein Tyrosine Phosphatases
- Zebrafish Biomedical Research Applications
University of Wisconsin–Madison
2019-2024
Abstract Neutrophils are cells at the frontline of innate immunity that can quickly activate effector functions to eliminate pathogens upon stimulation. However, little is known about metabolic adaptations power these functions. Here we show rapid alterations in neutrophils activation, particularly drastic reconfiguration around pentose phosphate pathway, which specifically and quantitatively coupled an oxidative burst. During this burst, switch from glycolysis-dominant metabolism a unique...
The emerging fungal pathogen Candida auris presents a global public health threat. Therapeutic options are often limited for this frequently drug-resistant pathogen, and mortality rates invasive disease high.
Hyphal growth is essential for host colonization during Aspergillus infection. The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition. However, how affects fungal susceptibility to immunity infection has not been investigated. Here, we use the larval zebrafish-Aspergillus model primary human neutrophils probe pathogenesis response antifungal drugs in vivo. deletion promotes clearance attenuates virulence wild-type...
Neutrophils are rapidly recruited to sites of infection and critical for pathogen clearance. Therapeutic use primary neutrophils has been limited, as they have a short lifespan not amenable genetic manipulation. Human induced pluripotent stem cells (iPSCs) can provide robust source infusion genetically tractable. However, current work indicated that dampened intracellular signaling limits iPSC-derived neutrophil (iNeutrophil) cellular activation antimicrobial response. Here, we show protein...
Aspergillus nidulans is an opportunistic fungal pathogen in patients with immunodeficiency, and virulence of A. isolates has mainly been studied the context chronic granulomatous disease (CGD), characterization clinical obtained from non-CGD remaining elusive. This study therefore carried out a detailed biological two (CIs), patient breast carcinoma pneumonia cystic fibrosis that underwent lung transplantation, compared them to reference, nonclinical FGSC A4 strain. Both CIs presented...
Neutrophils are primary phagocytes of the innate immune system that generate reactive oxygen species (ROS) and mediate host defense. Deficient phagocyte NADPH oxidase (PHOX) function leads to chronic granulomatous disease (CGD) is characterized by invasive infections, including those generally non-pathogenic fungus Aspergillus nidulans The role neutrophil ROS in this specific host-pathogen interaction remains unclear. Here, we exploit optical transparency zebrafish image effects on fungal...
Abstract Neutrophils are rapidly recruited to sites of infection and critical for pathogen clearance. Neutropenic patients at high risk fungal bacterial infections can benefit from granulocyte transfusion therapy. Human induced pluripotent stem cells (iPSCs) could provide a robust source neutrophil-like infusion as they be generated in large quantities do not require donor. However, dampened intracellular signaling limits their cellular activation response. Here, we show that engineer...
Abstract Aspergillus nidulans is an opportunistic fungal pathogen in patients with immunodeficiency and virulence of A. isolates has mainly been studied the context chronic granulomatous disease (CGD), characterization clinical obtained from non-CGD remaining elusive. This study therefore carried out a detailed biological two (CIs), patient breast carcinoma pneumonia cystic fibrosis that underwent lung transplantation, compared them to reference, non-clinical A4 strain. Both CIs presented...
Abstract The larval zebrafish is an increasingly popular host model for the study of Aspergillosis. visual accessibility, genetic resources, small size, and ease handling make larvae compatible with higher‐throughput investigation fungal virulence resistance mechanisms. This article provides protocols needed to prepare Aspergillus fumigatus spore inocula use microinjection infect hindbrain ventricle larvae. Furthermore, we include analyzing survival, immobilizing live imaging, suggestions...
Abstract Hyphal growth is essential for host colonization during Aspergillus infection. The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition. However, how affects fungal susceptibility to immunity infection has not been investigated. Here, we use the larval zebrafish- model primary human neutrophils probe pathogenesis response antifungal drugs in vivo . deletion promotes clearance attenuates virulence wild-type...
Abstract Neutrophils are primary cells of the innate immune system that generate reactive oxygen species (ROS) and mediate host defense. Deficient phagocyte NADPH oxidase (PHOX) function leads to chronic granulomatous disease (CGD) is characterized by invasive infections including those generally non-pathogenic fungus Aspergillus nidulans . The role neutrophil ROS in this specific host-pathogen interaction remains unclear. Here, we exploit optical transparency zebrafish image effects on...