A5025749732- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Biocrusts and Microbial Ecology
- 3D Printing in Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Vibrio bacteria research studies
- Vitamin C and Antioxidants Research
- Algal biology and biofuel production
- Minimally Invasive Surgical Techniques
- S100 Proteins and Annexins
- Protein Tyrosine Phosphatases
- Cell Adhesion Molecules Research
- Surgical Simulation and Training
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Abdominal Surgery and Complications
- Micro and Nano Robotics
- Polymer Surface Interaction Studies
University of Wisconsin–Madison
2023-2025
University of Minnesota
2019-2021
Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here, in both murine human PDA, we demonstrate that extracellular matrix architecture regulates cell extrusion subsequent invasion from intact structures through tumor-associated collagen signatures (TACS). This results early dissemination histologically premalignant lesions continual well-differentiated disease, it suggests TACS as a biomarker to aid the pathologic assessment of Furthermore, show...
is a ubiquitous soil and rhizosphere bacterium, but despite its abundance, the factors contributing to success in communities are poorly understood. Using model microbial community,
Neutrophils are rapidly recruited to sites of infection and critical for pathogen clearance. Therapeutic use primary neutrophils has been limited, as they have a short lifespan not amenable genetic manipulation. Human induced pluripotent stem cells (iPSCs) can provide robust source infusion genetically tractable. However, current work indicated that dampened intracellular signaling limits iPSC-derived neutrophil (iNeutrophil) cellular activation antimicrobial response. Here, we show protein...
Biofilms are three-dimensional structures containing one or more bacterial species embedded in extracellular polymeric substances. Although most biofilms stationary, Flavobacterium johnsoniae forms a motile spherical biofilm called zorb, which is propelled by its base cells and contains polysaccharide core. Here, we report the formation of spatially organized, motile, multispecies biofilms, designated “co-zorbs,” that distinguished core–shell structure. F. zorbs whose collect other transport...
Abstract Leukocytes forge paths through interstitial spaces by exerting forces to overcome confining mechanical pressures provided surrounding cells. While such cues regulate leukocyte motility, engineering an in vitro system that models the deformable cellular environment encountered vivo has been challenging. Here, microchannels are constructed with a liquid‐liquid interface exerts similar cells tissues, and thus, is cell‐generated forces. Consequently, balance between migratory...
Intra-abdominal organ and vascular injuries during laparoscopic trocar placement remain a significant cause for surgical complications procedures. These can arise if the surgeon deviates from proper axis, requiring additional applied force to obliquely traverse abdominal wall. This increase in application increases risk of internal vessel damage immediately after entrance peritoneal cavity. To mitigate this risk, our group designed that provides real-time feedback deviation insertion force,...
Leukocytes navigate through interstitial spaces resulting in deformation of both the motile leukocytes and surrounding cells. Creating an vitro system that models deformable cellular environment encountered vivo has been challenging. Here, we engineer microchannels with a liquid-liquid interface exerts confining pressures (200-3000 Pa) similar to cells tissues, and, thus, is by cell generated forces. Consequently, balance between migratory cell-generated interfacial determines degree...
Abstract Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here in both murine human PDA we demonstrate that extracellular matrix architecture regulates cell extrusion subsequent invasion from intact structures through Tumor-Associated Collagen Signatures (TACS), resulting early dissemination histologically pre-malignant lesions continual well-differentiated Furthermore, show pancreatitis results invasion-conducive architectures, thus priming the stroma...
ABSTRACT Flavobacterium johnsoniae is a ubiquitous soil and rhizosphere bacterium, but despite its abundance, the factors contributing to success in communities are poorly understood. Using model microbial community, T he H itchhikers O f Rhizosphere (THOR), we determined effects of colonization on fitness F. community. Insertion sequencing (INSeq), massively parallel transposon mutant screen, sterile sand identified 25 genes likely be important for surface colonization. We constructed...
Abstract Biofilms are three-dimensional structures containing one or more bacterial species embedded in extracellular polymeric substances. Although most biofilms stationary, Flavobacterium johnsoniae forms a motile spherical biofilm called zorb, which is propelled by its base cells and contains polysaccharide core. Here, we report formation of spatially organized, motile, multispecies biofilms, designated “co-zorbs,” that distinguished core-shell structure. F. zorbs whose collect other...