A5052148064- Antifungal resistance and susceptibility
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Parvovirus B19 Infection Studies
- Fungal and yeast genetics research
- Dermatological and COVID-19 studies
- Cystic Fibrosis Research Advances
- Peptidase Inhibition and Analysis
- Glutathione Transferases and Polymorphisms
- Fungal Infections and Studies
- Mycorrhizal Fungi and Plant Interactions
- Polyamine Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Redox biology and oxidative stress
- Effects of Environmental Stressors on Livestock
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Insect and Pesticide Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Prenatal Screening and Diagnostics
- Animal health and immunology
- Meat and Animal Product Quality
- Plant Pathogens and Fungal Diseases
National University of Ireland, Maynooth
2015-2025
Trinity College
2021
University of Indianapolis
2020
Indiana University – Purdue University Indianapolis
2020
Kildare Education Centre
2018
Wichita State University
2016
University of Manchester
2012
Cancer Research UK Manchester Institute
2012
Queen's University Belfast
2011
Royal Stoke University Hospital
2011
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded biosynthetic gene clusters. Information about these clusters, is currently dispersed throughout the literature, making it difficult exploit. To facilitate consistent systematic deposition retrieval data on we propose Minimum a Biosynthetic Gene cluster (MIBiG) standard.
Conventional in vivo assays to determine the relative pathogenicity of yeast isolates rely upon use a range mammalian species. The purpose work presented here was investigate possibility using an insect (Galleria mellonella) as model system for testing. haemolymph G. mellonella larvae inoculated with PBS containing different concentrations stationary phase yeasts genus Candida by injection at last pro-leg. Larvae were incubated 30°C and monitored over 72 hours. Results indicate that can be...
Gliotoxin, and other related molecules, are encoded by multi-gene clusters biosynthesized fungi using non-ribosomal biosynthetic mechanisms. Almost universally described in terms of its toxicity towards mammalian cells, gliotoxin has come to be considered as a component the virulence arsenal Aspergillus fumigatus. Here we show that deletion single gene, gliT, cluster two A. fumigatus strains, rendered organism highly sensitive exogenous completely disrupted secretion. Addition glutathione...
Abstract Armillaria species are both devastating forest pathogens and some of the largest terrestrial organisms on Earth. They forage for hosts achieve immense colony sizes via rhizomorphs, root-like multicellular structures clonal dispersal. Here, we sequenced analysed genomes four performed RNA sequencing quantitative proteomic analysis invasive reproductive developmental stages A. ostoyae . Comparison with 22 related fungi revealed a significant genome expansion in , affecting several...
The secretion and activation of the major cathepsin L1 cysteine protease involved in virulence helminth pathogen Fasciola hepatica was investigated. Only fully processed active mature enzyme can be detected medium which adult F. are cultured. However, immunocytochemical studies revealed that inactive procathepsin is packaged secretory vesicles epithelial cells line parasite gut. These observations suggest processing occurs following from these into acidic gut lumen. Expression 37-kDa Pichia...
Sixteen, spring-born, single suckled, castrated male calves of Limousin x Holstein-Friesian and Simmental dams respectively, were used to investigate the effect weaning on total leukocyte differential counts, neutrophil functional activity, lymphocyte immunophenotypes, acute phase protein response. Calves grazed with their until end grazing season when they housed in a slatted floor shed. On day housing, assigned treatment, (i) abruptly weaned (W: n = 8) or (ii) non-weaned (controls) (C: 8)....
Armillaria mellea is a major plant pathogen. Yet, no large-scale "-omics" data are available to enable new studies, and limited experimental models investigate basidiomycete pathogenicity. Here we reveal that the A. genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). Tandem mass spectrometry identified 921 mycelial (n = 629 unique) secreted 183 proteins. Almost 100 proteins were either species-specific or previously unidentified at protein...
Abstract Background The effect of pre-weaning concentrate supplementation on peripheral distribution leukocytes, functional activity neutrophils, acute phase protein response, metabolic and behavioural performance abruptly weaned housed beef calves was investigated. Calves were grazed with their dams until the end grazing season when they (day (d) 0) in a concrete slatted floor shed, offered grass silage ad libitum plus supplementary concentrates. Twenty-six days prior to weaning housing, 20...
Many cystic fibrosis (CF) airway infections are considered to be polymicrobial and microbe-microbe interactions may play an important role in disease pathology. Pseudomonas aeruginosa Aspergillus fumigatus the most prevalent bacterial fungal pathogens isolated from CF airway, respectively. We have previously shown that patients co-colonized with these had comparable outcomes those chronically colonized P. aeruginosa. Our objective was examine between A. aeruginosa, specifically effects of...
Abstract Ergothioneine (EGT; 2-mercaptohistidine trimethylbetaine) is a trimethylated and sulphurised histidine derivative which exhibits antioxidant properties. Here we report that deletion of Aspergillus fumigatus egtA (AFUA_2G15650), encodes trimodular enzyme, abrogated EGT biosynthesis in this opportunistic pathogen. biosynthetic deficiency A. significantly reduced resistance to elevated H 2 O menadione, respectively, impaired gliotoxin production resulted attenuated conidiation....
During sporulation of Saccharomyces cerevisiae, meiosis is followed by encapsulation haploid nuclei within multilayered spore walls. Completion the late events program requires SPS1 gene. This developmentally regulated gene, which expressed as cells are nearing end meiosis, encodes a protein with homology to serine/threonine kinases. The catalytic domain Sps1 44% identical kinase yeast Ste20, involved in pheromone-induced signal transduction pathway. Cells MATa/MAT alpha sps1/sps1 strain...
The purpose of this study was to develop a reliable molecular procedure for the detection Escherichia coli in milk.Robust and expeditious DNA extraction PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) biotin-labelled amplicons facilitate optimal E. DNA.It found that 5 colony-forming units (cfu) could be detected per reaction PCR-ELISA system, equating sensitivity 100 cfu ml(-1) pasteurized milk.This approach should evaluation milk contamination enable rapid...
Abstract Background The molecular mechanisms by which stress induces the development of pathologies remains unclear, although it is recognised that one major factors affecting health as a consequence involvement neuroendocrine system. In cattle, number necessary husbandry practices have been shown to activate response, yet very little known about impact these at level. objectives study were characterise, in male and female beef calves, immune response weaning bovine leukocytes physiological...
ABSTRACT Mechanistic studies on gliotoxin biosynthesis and self-protection in Aspergillus fumigatus , both of which require the oxidoreductase GliT, have revealed a rich landscape highly novel biochemistries, yet key aspects this complex molecular architecture remain obscure. Here we show that an A. Δ gliA strain is completely deficient secretion but still retains ability to efflux bisdethiobis(methylthio)gliotoxin (BmGT). This correlates with significant increase sensitivity exogenous...