A5080769340- Ruminant Nutrition and Digestive Physiology
- Microbial Natural Products and Biosynthesis
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Meat and Animal Product Quality
- Reproductive Physiology in Livestock
- Animal Behavior and Welfare Studies
- Antifungal resistance and susceptibility
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Rabbits: Nutrition, Reproduction, Health
- Fungal Biology and Applications
- Animal health and immunology
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Infectious Diseases and Mycology
- Marine Sponges and Natural Products
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Nutritional Studies and Diet
- Lipid Membrane Structure and Behavior
- Phytochemical compounds biological activities
University College Dublin
2011-2024
Chemical Synthesis Lab
2012-2013
IBM (United States)
2003
University of Cambridge
1991-1996
Trinity College Dublin
1987-1991
Lyon College
1977
University of Wisconsin–Madison
1975
University of Illinois System
1967
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded biosynthetic gene clusters. Information about these clusters, is currently dispersed throughout the literature, making it difficult exploit. To facilitate consistent systematic deposition retrieval data on we propose Minimum a Biosynthetic Gene cluster (MIBiG) standard.
The effects of grazed grass, grass silage, or concentrates on fatty acid composition and conjugated linoleic (cis-9, trans-11-18:2; CLA) concentrations i.m. fat steers fed to achieve similar carcass growth rates were investigated. Fifty divided into 10 blocks based body weight assigned at random from within one five dietary treatments. experimental rations offered daily for 85 d preceding slaughter 1) silage ad libitum intake plus 4 kg concentrate, 2) 8 concentrate 1 hay, 3) 6 DM 5 4) 12 2.5...
The macrocyclic polyketides rapamycin and FK506 are potent immunosuppressants that prevent T-cell proliferation through specific binding to intracellular protein receptors (immunophilins). cloning alteration of the biosynthetic genes for these might allow biosynthesis clinically valuable analogues. We report here three clustered polyketide synthase responsible in Streptomyces hygroscopicus together encode 14 homologous sets enzyme activities (modules), each catalyzing a round chain...
Getting down to specifics: Key amino acid residues were found correlate with ketoreductase domain stereospecificity in modular polyketide synthases. These may allow alcohol stereochemistry (see scheme; ACP, acyl carrier protein) polyketides be predicted from sequences. The results also suggest that synthase dehydratase domains have a preference for 3hydroxyacyl substrates the same as (3R)-hydroxyacyl chains used by dehydratases fatty Supporting information this article is available on WWW...
During assembly of complex polyketide antibiotics like erythromycin A, molecular recognition by the multienzyme synthase controls stereochemical outcome as each successive methylmalonyl-coenzyme A (CoA) extender unit is added. Acylation purified erythromycin-producing has shown that all six acyltransferase domains have identical stereospecificity for their normal substrate, (2 S )-methylmalonyl-CoA. In contrast, configuration methyl-branched centers in product, are derived from...
: Some feed ingredients bind more acid in the stomach than others and for this reason may be best omitted from pig starter foods if gastric acidity is to promoted. The objective of study was measure acid-binding capacity (ABC) commonly used foods. Ingredients were categorised as follows: (i) milk products (n = 6), (ii) cereals 10), (iii) root pulp 5), (iv) vegetable proteins 11), (v) meat fish meal 2), (vi) medication 3), (vii) amino acids 4), (viii) minerals 16), (ix) salts (x) 10). A 0.5 g...
The ery A region of the erythromycin biosynthetic gene cluster Saccharopolyspora erythraea has previously been shown to contain three large open reading frames (ORFs) that encode components 6‐deoxyerythronolide B synthase (DEBS). Polyclonal antibodies were raised against recombinant proteins obtained by overexpression 3′ regions ORF2 and ORF3 genes. In Western blotting experiments, each antiserum reacted strongly with a different high molecular weight protein in extracts...
Amphotericin B is a medically important antifungal antibiotic that also active against human immunodeficiency virus, Leishmania parasites, and prion diseases. The therapeutic use of amphotericin restricted by severe side effects can be moderated liposomal formulation or structural alteration. Chemical modification has shown suppression charge on the exocyclic carboxyl group substantially reduces toxicity. We report targeted deletions amphN cytochrome P450 gene from chromosome...
The domain structure of the 6-deoxyerythronolide B synthase 1 component erythromycin-producing polyketide from Saccharopolyspora erythraea has been investigated using limited proteolysis and active-site labeling. Trypsin, elastase, endoproteinase Glu-C, Arg-C were used to cleave multienzyme, sizes resulting fragments assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. location within primary was established N-terminal sequence analysis. cleavage pattern followed boundaries...
A parallel application benefits from scheduling policies that include a global perspective of the application's process working set. As interactions among cooperating processes increase, mechanisms to ameliorate waiting within one or more become important. In particular, collective operations such as barriers and reductions are extremely sensitive even usually harmless events context switches members For last 18 months, we have been researching impact random short-lived interruptions...
Four experiments were undertaken to examine the effect of feeding postweaning diets as dry pelleted feed, fresh liquid acidified and fermented feed on pig performance from weaning (26 d) harvest. In Exp. 1 (n = 12 replicates) 2 10 replicates), treatments 1) 2) feed. 1, kg starter diet (16.7 MJ DE/kg 1.6% lysine) per 5 transition 1.5% followed by a weaner (14.0 1.36% offered 27 d after weaning. 3 8 3) 4 2, 3, 4, (16.1 1.74% 6 (15.3 was All balanced for boars gilts ad libitum consumption....
The C‐terminal region of a multifunctional polypeptide from the 6‐deoxyerythronolide B synthase Saccharopolyspora erythraea is predicted to contain an acyl carrier protein and thioesterase or acyltransferase activity [Cortes, J., Haydock, S. F., Roberts, G. A., Bevitt, D. J. & Leadlay, P. F. (1990) Nature 348 , 176–178]. Site‐directed mutagenesis by means polymerase chain reaction was used construct efficient pT7‐based expression plasmid for this domain. recently developed technique...
A number of antifungal drugs are based on polyene macrolides that cause severe side effects. Most these compounds contain a single aminodeoxysugar, D-mycosamine. Toxicity can be reduced by increasing the extent glycosylation. The aromatic heptaene 67-121C and two analogues degenerate nystatin have second sugar attached to C4′ hydroxyl mycosamine. Another analogue has L-digitoxose as C35 macrolactone ring. pentaene selvamicin 4-O-methyl-L-digitoxose at C27, equivalent position. To assist...
Abstract The aim of this study was to assess the effect weaning weight and post-weaning diet on performance weaned pigs. In experiment 1, 30 litters with more than 10 pigs born alive per litter were selected. At 11 days age, average for removed from 15 so that eight remained sow. These given access creep food (16·5 MJ digestible energy (DE) kg 18·7 g/kg lysine). remaining left complete not food. Pigs at 28 age pairs (a male a female littermate similar weight) formed each (no. = 54 pairs)....