A5070853383- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Marine Sponges and Natural Products
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical synthesis and alkaloids
- Microbial Metabolism and Applications
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Phytochemical compounds biological activities
- Alkaloids: synthesis and pharmacology
- Cancer therapeutics and mechanisms
- Catalytic C–H Functionalization Methods
- Metabolomics and Mass Spectrometry Studies
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Synthetic Organic Chemistry Methods
- Enzyme Catalysis and Immobilization
- Nematode management and characterization studies
- Bioactive Compounds and Antitumor Agents
- 14-3-3 protein interactions
- Organic Chemistry Cycloaddition Reactions
- CRISPR and Genetic Engineering
Chinese Academy of Sciences
2014-2025
University of Chinese Academy of Sciences
2013-2025
Ecologie Microbienne Lyon
2025
Czech Academy of Sciences, Institute of Microbiology
2025
Microbiology Institute of Shaanxi
2013-2024
Institute of Microbiology
2013-2024
Daqing Oilfield General Hospital
2024
The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
2024
Peking University
2023
Ningbo University
2023
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded biosynthetic gene clusters. Information about these clusters, is currently dispersed throughout the literature, making it difficult exploit. To facilitate consistent systematic deposition retrieval data on we propose Minimum a Biosynthetic Gene cluster (MIBiG) standard.
Significance Leinamycin (LNM) is a promising anticancer drug lead, yet no analog has been isolated since its discovery nearly 30 y ago. By mining bacterial genomes, we discovered 49 potential producers of LNM-type natural products, the structural diversity which was predicted based on bioinformatics and confirmed by in vitro characterization selected enzymes elucidation guangnanmycins weishanmycins. These findings demonstrate power discovery-based approach to combinatorial biosynthesis for...
Catalases play a key role in the defense against oxidative stress bacteria by catalyzing decomposition of H 2 O . In addition, catalases are also involved multiple cellular processes, such as cell development and differentiation, well metabolite production. However, little is known about abundance, diversity, distribution bacteria. this study, we systematically surveyed classified homologs three catalase families from 2,634 bacterial genomes. It was found that both typical Mn-catalase could...
Significance This work addresses the molecular basis for interspecies signaling effects of antibiotics, which have been a controversial but potentially significant emerging topic over last few years. The “pseudo” gamma-butyrolactone (GBL) receptor (i.e., those GBL homologues often found in Streptomyces genomes, apparently not binding or responding to GBLs), ScbR2, was identified as JdB. It has an extraordinary ability bind and respond exogenous angucyclines, well be able directly regulate...
A conserved pair of oxidases is characterized as nature's machinery for benzofluorenone formation.
Tetracycline (TC) family natural products have attracted significant attention due to their diverse chemical structures and important role in drug development. As one of the most successful classes drugs, TC antibiotics been used clinically for over 70 years remain crucial treating infections. Despite importance, systematic exploration novel has limited, leaving molecular landscape poorly understood hindering further development these compounds therapeutic applications. Here, we developed a...
Jadomycin production is under complex regulation in Streptomyces venezuelae. Here, another cluster-situated regulator, JadR*, was shown to negatively regulate jadomycin biosynthesis by binding four upstream regions of jadY, jadR1, jadI and jadE jad gene cluster respectively. The transcriptional levels target genes JadR* increased significantly ΔjadR*, confirming that these were directly repressed JadR*. B (JdB) its biosynthetic intermediates 2,3-dehydro-UWM6 (DHU), dehydrorabelomycin (DHR) A...
The guangnanmycins (GNMs) belong to a small group of natural products featuring 1-aminocyclopropane-1-carboxylic acid (ACC) moiety. While extensively studied in plants, ACC biosynthesis bacteria remains poorly understood. Here we report inactivation gnmY vivo and biochemical characterization GnmY vitro, assigning as the first bacterial free synthase that catalyzes synthesis from S-adenosyl methionine. is activated by GnmS subsequently incorporated into GNM scaffold hybrid nonribosomal...
C-C bond ring cleaving oxygenases represent a unique family of enzymes involved in the B cleavage reaction only observed atypical angucycline biosynthesis. is key leading to dramatic divergence final structures angucyclines. Here, we present crystal structure AlpJ, first this enzymes. AlpJ has been verified as enzyme catalyzing kinamycin The monomer resembles dimeric ferredoxin-like proteins. N- and C-terminal halves are homologous, both contain putative hydrophobic substrate binding pocket...
We confirm the previously revised stereochemistry of spiroviolene by X-ray crystallographically characterizing a hydrazone derivative 9-oxospiroviolane, which is synthesized hydroboration/oxidation followed oxidation resultant hydroxy group. An unexpected thermal boron migration occurred during hydroboration process that resulted in production mixture 1α-hydroxyspiroviolane, 9α- and 9β-hydroxyspiroviolane after oxidation. The assertion
Leinamycin (LNM) is biosynthesized by a hybrid nonribosomal peptide synthetase (NRPS)–acyltransferase (AT)-less type I polyketide synthase (PKS). Characterization of LnmI revealed ketosynthase (KS)–acyl carrier protein (ACP)–KS domains at the NRPS–PKS interface. Inactivation KS domain or ACP in vivo abolished LNM production, and can be phosphopantetheinylated vitro. The KS–ACP–KS architecture represents new mechanism for functional crosstalk between NRPS AT-less PKS biosynthesis...
Nature forms S-S bonds by oxidizing two sulfhydryl groups, and no enzyme installing an intact hydropersulfide (-SSH) group into a natural product has been identified to date. The leinamycin (LNM) family of products features bonds, previously we reported SH domain (LnmJ-SH) within the LNM hybrid nonribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) assembly line as cysteine lyase that plays role in sulfur incorporation. Here report characterization S-adenosyl methionine...
Abstract The dehydratase domains (DHs) of the iso‐migrastatin (iso‐MGS) polyketide synthase (PKS) were investigated by systematic inactivation DHs in module‐6, ‐9, ‐10 MgsF (i.e., DH6, DH9, DH10) and module‐11 MgsG DH11) vivo, followed structural characterization metabolites accumulated mutants, biochemical DH10 vitro, using substrate mimics with varying chain lengths. These studies allowed us to assign functions for all four DHs, identifying as dedicated that catalyzes dehydration C17...
The ever-growing drug resistance problem worldwide highlights the urgency to discover and develop new drugs. Microbial natural products are a prolific source of Genome sequencing has revealed tremendous amount uncharacterized product biosynthetic gene clusters (BGCs) encoded within microbial genomes, most which cryptic or express at very low levels under standard culture conditions. Therefore, developing effective strategies awaken these BGCs is great interest for discovery. In this study,...
An isopentenol utilization pathway-based method for the investigation of cyclization mechanism terpene cyclases (TCs) is developed. By feeding deuterium-labeled prenols/isoprenols in combination with unlabeled ones to engineered E. coli hosts, products certain deuterium labeling patterns at hydrogen-bearing positions were obtained that can be used deducing processes, especially those steps involving stereoselective hydride/proton shifts. Different types TCs varied origins biosynthesis six...
Efficient utilization of both glucose and xylose, the two most abundant sugars in biomass hydrolysates, is one main objectives biofermentation with lignocellulosic materials. The xylose commonly inhibited by glucose, which known as catabolite repression (GCR). Here, we report a GCR-based dynamic control (GCR-DC) strategy aiming at better co-utilization decoupling cell growth biosynthesis riboflavin product. Using thermophilic strain Geobacillus thermoglucosidasius DSM 2542 host, constructed...
Abstract The dehydratase domains (DHs) of the iso‐migrastatin (iso‐MGS) polyketide synthase (PKS) were investigated by systematic inactivation DHs in module‐6, ‐9, ‐10 MgsF (i.e., DH6, DH9, DH10) and module‐11 MgsG DH11) vivo, followed structural characterization metabolites accumulated mutants, biochemical DH10 vitro, using substrate mimics with varying chain lengths. These studies allowed us to assign functions for all four DHs, identifying as dedicated that catalyzes dehydration C17...