A5034872698- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Enzyme Structure and Function
- Prenatal Screening and Diagnostics
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Heat shock proteins research
- Soybean genetics and cultivation
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Mathematical Biology Tumor Growth
- Microbial Metabolic Engineering and Bioproduction
- Hedgehog Signaling Pathway Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Aquaculture Nutrition and Growth
- Silk-based biomaterials and applications
- Endoplasmic Reticulum Stress and Disease
- Plant Pathogenic Bacteria Studies
- Genetic Mapping and Diversity in Plants and Animals
- Genetics, Aging, and Longevity in Model Organisms
- Invertebrate Immune Response Mechanisms
Southwest University
2020-2024
ShanghaiTech University
2022-2024
Hebei Chemical and Pharmaceutical College
2022
Zhejiang A & F University
2022
State Key Laboratory of Silkworm Genomic Biology
2020
Regenex Pharmaceuticals (China)
2019
Soochow University
2014-2015
Zhangjiagang First People's Hospital
2014
Harbin Normal University
2014
To investigate the mechanism by which miR-19a is up-regulated in gastric cancer (GC), plays an oncogenic role.In present study, we investigated role of tissues as well two GC cell lines. In vivo, detected basal expression level using real-time reverse transcription-PCR (RT-PCR), and relevance between clinicopathological information was analyzed. vitro, ectopically expressed overexpression knock-down strategies.Overexpression significantly associated with metastasis inferior overall...
Hickory (Carya cathayensis) is a critical tree species of the genus Carya from Juglandaceae family that contains nutrient-rich nuts. Due to large-scale soil degradation, pests and diseases hickory are becoming more serious. Thaumatin-like proteins (TLPs) vital involved in complex defense process plant pathogens. In this study, 40 CcTLP genes were identified genome-widely phylogenetically grouped into three subfamilies. The sequence CcTLPs had conservative pattern, such as eight stable...
The present study aimed to investigate the role of PTCH1 methylation in gastric carcinogenesis and therapeutic effect inhibitor, 5-aza-2'-deoxycytidine (5-aza-dC), treatment cancer. Total RNA was extracted from 20 cancer tissues, their corresponding adjacent normal tissues a AGS cell line. mRNA expression detected by quantitative PCR, promoter examined methylation-specific PCR. cells were treated with 5-Aza-dC; apoptosis cycle flow cytometry, level observed. negatively correlated line...
DNA methylation is mainly catalyzed by three methyltransferase (DNMT) proteins in mammals. Usually DNMT1 considered the primary DNMT for maintenance methylation, whereas DNMT3A and DNMT3B function de novo methylation. Interestingly, we found exerted postimplantation mouse embryos. Together with DNMT1, they maintained at some pluripotent genes lineage marker genes. Germline-derived imprinting control regions (ICRs) stably most ICRs Surprisingly, was increased five after implantation, two...
Efficient utilization of both glucose and xylose, the two most abundant sugars in biomass hydrolysates, is one main objectives biofermentation with lignocellulosic materials. The xylose commonly inhibited by glucose, which known as catabolite repression (GCR). Here, we report a GCR-based dynamic control (GCR-DC) strategy aiming at better co-utilization decoupling cell growth biosynthesis riboflavin product. Using thermophilic strain Geobacillus thermoglucosidasius DSM 2542 host, constructed...
ZFP57 and ZFP445 maintain genomic imprinting in mouse embryos. We found DNA methylation was lost at most examined control regions (ICRs) Zfp57 mutant ES cells, which could not be prevented by the elimination of three TET proteins. To elucidate maintenance mechanisms, we generated clones lacking major methyltransferases (DNMTs). Intriguingly, DNMT3A DNMT3B were essential for a subset ICRs cells although DNMT1 maintained known ICRs. These similarly observed after extended culture....
Zfp57 has both maternal and zygotic functions in mouse. It maintains genomic imprinting at most known imprinted regions controls allelic expression of the target genes mouse embryos. The DNA methylation imprint many control (ICRs) is lost when are absent maternal–zygotic mutant Interestingly, we found that a few ICRs was partially without heterozygous embryos derived from homozygous female mice. This suggests essential for maintenance small subset effect applied to switch as well levels...
Fish sex is largely influenced by steroid hormones, especially hormones. Here, we established a hormone-free genetic model mutation of cyp11a1 in Nile tilapia, which was confirmed EIA assay. Gonadal phenotype and transcriptome analyses showed that the XX mutants displayed reversal from female to male but with defective spermatogenesis. Despite reversal, aromatase encoding gene cyp19a1a continuously expressed gonads mutants, might be caused androgen deficiency. Whole-mount fluorescence situ...
Histotripsy has recently gained FDA approval for the treatment of liver cancer. However, there is limited clinical understanding how different histotripsy doses can generate varying levels anti-tumor response, thereby impacting patient survival. This work evaluates impact dosing on immune response and tumor-free survival outcomes in an orthotopic rodent model. Orthotopic McA-RH7777 tumors generated immunocompetent Sprague Dawley rats were ablated using doses: dose 1 (50Hz PRF, 50 pulses per...
Gene functions can be assessed in mouse embryonic stem (ES) cells and mutant mice derived from ES cells. Here, we describe an approach for efficient isolation of the clones carrying deletion mutations at target genes by CRISPR-Cas9. Two sgRNAs against a gene are co-expressed with puromycin-resistant through co-transfection followed transient puromycin selection. Deletion identified PCR individual that picked puromycin-selected