A5003878253- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- HIV Research and Treatment
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- CRISPR and Genetic Engineering
- interferon and immune responses
- Hepatitis C virus research
- Animal Nutrition and Physiology
- Prenatal Screening and Diagnostics
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Parasitic infections in humans and animals
- Endoplasmic Reticulum Stress and Disease
- Congenital Anomalies and Fetal Surgery
- Immune cells in cancer
- Parasitic Infections and Diagnostics
- Heat shock proteins research
- Genetic Syndromes and Imprinting
- Molecular Biology Techniques and Applications
- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- DNA Repair Mechanisms
Shanghai Academy of Agricultural Sciences
2025
First People's Hospital of Yunnan Province
2025
Shanghai Ocean University
2024
First Affiliated Hospital of Anhui Medical University
2023
ShanghaiTech University
2019-2023
Anhui Medical University
2023
Shanxi Agricultural University
2009-2022
Northwest University
2021
Chinese National Human Genome Center
2012-2020
Chinese National Human Genome Center at Shanghai
2012-2020
Tubulointerstitial fibrosis is a common consequence of various kidney diseases that lead to end-stage renal failure, and lymphocyte infiltration plays an important role in fibrosis. We previously found depletion cluster differentiation 8⁺ (CD8⁺) T cells increases following ureteric obstruction, interferon-γ (IFN-γ)-expressing CD8⁺ contribute this process. are cytotoxic cells; however, whether their effect reduces remains unknown. This study showed isolated from obstructed mRNA expression the...
Eukaryotic stress granules (SGs) are highly dynamic assemblies of untranslated mRNAs and proteins that form through liquid-liquid phase separation (LLPS) under cellular stress. SG formation elimination process is a conserved strategy to promote cell survival, although the precise regulation this poorly understood. Here, we screened six E3 ubiquitin ligases present in SGs identified TRIM21 (tripartite motif containing 21) as central regulator homeostasis enriched cells arsenite-induced...
Background We explore sphingolipid-related genes (SRGs) in skin melanoma (SKCM) to develop a prognostic indicator for patient outcomes. Dysregulated lipid metabolism is linked aggressive behavior various cancers, including SKCM. However, the exact role and mechanism of sphingolipid remain partially understood. Methods integrated scRNA-seq data from patients sourced GEO database. Through utilization Seurat R package, we successfully identified distinct gene clusters associated with survival...
With the use of an intrachromosomal inverted-repeat as a recombination reporter we have previously shown that mitotic is dependent on RAD52 gene. However, was found to be reduced only 4-fold by mutation RAD51, which encodes homolog bacterial RecA proteins. A rad51, strain containing mutagenized identify components RAD51-independent pathway. One identified, rad59, 1200-fold in presence rad51 mutation, but 4- 5-fold wild-type background. Thus and rad59 mutations reduce synergistically. The...
Protein fate in higher eukaryotes is controlled by three complexes that share conserved architectural elements: the proteasome, COP9 signalosome, and eukaryotic translation initiation factor 3 (eIF3). Here we reconstitute 13-subunit human eIF3 Escherichia coli , revealing its structural core to be eight subunits with orthologues proteasome lid complex signalosome. This binds small (40S) ribosomal subunit, factors involved mRNA cap-dependent initiation, hepatitis C viral (HCV) internal...
HIV replication requires nuclear export of unspliced and singly spliced viral transcripts. Although a unique RNA structure has been proposed for the Rev-response element (RRE) responsible mRNA export, how it recruits multiple Rev proteins to form an complex unclear. We show here that initial binding RRE triggers tertiary structural changes, enabling further rapid formation complex. Analysis Rev-RRE assembly pathway using SHAPE-Seq small-angle X-ray scattering (SAXS) reveals two major steps...
The 3′ untranslated region (3′UTR) of hepatitis C virus (HCV) messenger RNA stimulates viral translation by an undetermined mechanism. We identified a high affinity interaction, conserved among different HCV genotypes, between the 3′UTR and host ribosome. interacts with 40S ribosomal subunit proteins residing primarily in localized on solvent-accessible surface near entry exit sites. This partially overlaps site where internal ribosome was found to bind, site-40S interaction being dominant....
MicroRNAs (miRNAs) are important post-transcriptional regulators which control growth and development in eukaryotes. The cestode Echinococcus granulosus has a complex life-cycle involving different stages but the mechanisms underpinning this development, including involvement of miRNAs, remain unknown. Using Illumina next generation sequencing technology, we sequenced at genome-wide level three small RNA populations from adult, protoscolex cyst membrane E. granulosus. A total 94 pre-miRNA...
HIV-1 Tat hijacks the human superelongation complex (SEC) to promote proviral transcription. Here we report 5.9 Å structure of TAR in with and AFF4, CDK9, CycT1. The central loop contacts CycT1 Tat-TAR recognition motif (TRM) second Zn2+-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized despite not touching RNA, explaining how it enhances binding SEC 50-fold. RNA SHAPE SAXS data were used help model extended (Tat Arginine-Rich Motif) ARM, which enters...
The Colletotrichum fructicola (C. fructicola) is a hemibiotrophic fungus, which causes devastating anthracnose in strawberry. At present, the resistance mechanism to C. remains poorly understood. Here, we used RNA-sequencing and liquid chromatography-mass spectrometry (LC-MS) metabolomics excavate molecular of strawberry fructicola. differentially accumulated metabolites (DAMs) expressed genes (DEGs) were screened at different stages after infection susceptible 'Benihoppe' resistant cultivar...
HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of large excess cytosolic human RNAs. As assembles on plasma membrane, genome is enriched relative to cellular RNAs by an unknown mechanism. We used minimal system consisting purified RNAs, recombinant giant unilamellar vesicles recapitulate selective packaging 5' untranslated region presence competitor RNA. Mutations CA-CTD domain which subtly affect self-assembly abrogated selectivity. further found that tRNA...
The constitutive splicing factor ASF/SF2 has been shown to affect the choice between alternative splice sites by favoring proximal as opposed distal choice. HnRNP A1 antagonizes promoting for competing 5′ sites. We have tested in vivo effects of these proteins on 3′ site choices. Cotransfection a dihydrofolate reductase-calcitonin chimeric construct together with plasmid specifying SR protein into cells several mammalian lines increased use site, resulting inclusion terminal calcitonin exon....
Abstract With the use of an intrachromosomal inverted repeat as a recombination reporter, we have shown that mitotic is dependent on RAD52 gene, but reduced only fivefold by mutation RAD51. RAD59, component RAD51-independent pathway, was identified previously screening for mutations inverted-repeat in rad51 strain. Here describe rad52 mutation, rad52R70K, also synergistically background. The phenotype rad52R70K strain, which includes weak γ-ray sensitivity, fourfold reduction rate...
Significance This study provides further proof for ZFP57 as a master regulator of genomic imprinting since it maintains DNA methylation at most known imprinted regions and regulates allelic expression the corresponding genes. We propose two alternate switch models ZFP57-dependent regulation parent-of-origin–dependent genes based on quantitative analyses Our results also shed light mechanistic link between imprint monoallelic The indicate that clustered through maintenance control regions.
Down syndrome (DS) is caused by triplication of Human chromosome 21 (Hsa21) and associated with an array deleterious phenotypes, including mental retardation, heart defects immunodeficiency. Genome-wide expression patterns uncultured peripheral blood cells are useful to understanding DS-associated immune dysfunction. We used a Exon microarray characterize gene in derived from DS individuals age-matched controls two age groups: neonate (N) child (C). A total 174 transcript clusters...
Abstract Mumps virus (MuV) is a highly contagious human pathogen and frequently causes worldwide outbreaks despite available vaccines. Similar to other mononegaviruses such as Ebola rabies, MuV uses single-stranded negative-sense RNA its genome, which enwrapped by viral nucleoproteins into the helical nucleocapsid. The nucleocapsid acts scaffold for genome condensation template replication transcription. Conformational changes in are required switch between different activities, but...