A5058713062- Parasitic infections in humans and animals
- Neurogenesis and neuroplasticity mechanisms
- Parasitic Infections and Diagnostics
- Congenital Anomalies and Fetal Surgery
- Marine and coastal plant biology
- Parasite Biology and Host Interactions
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Coastal wetland ecosystem dynamics
- Bacteriophages and microbial interactions
- Toxoplasma gondii Research Studies
- Cancer-related molecular mechanisms research
- Parasites and Host Interactions
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Spinal Dysraphism and Malformations
- MicroRNA in disease regulation
- Marine Biology and Ecology Research
- Nerve injury and regeneration
- Neonatal and fetal brain pathology
- Developmental Biology and Gene Regulation
- Bryophyte Studies and Records
- Seaweed-derived Bioactive Compounds
- Genetic Neurodegenerative Diseases
- Olfactory and Sensory Function Studies
Zhongshan Hospital
2018-2025
State Key Laboratory of Medical Neurobiology
2014-2025
Fudan University
2014-2025
Xinjiang Academy of Animal Science
2013-2024
Tongji University
2024
University of California, San Francisco
2024
University of Hong Kong
2024
North China University of Science and Technology
2024
Children's Hospital of Fudan University
2020-2022
Shandong Jiaotong University
2021
Cystic echinococcosis (hydatid disease), caused by the tapeworm E. granulosus, is responsible for considerable human morbidity and mortality. This cosmopolitan disease difficult to diagnose, treat control. We present a draft genomic sequence worm comprising 151.6 Mb encoding 11,325 genes. Comparisons with genome sequences from other taxa show that granulosus has acquired spectrum of genes, including EgAgB family, whose products are secreted parasite interact redirect host immune responses....
Abstract Mouse cortical radial glial cells (RGCs) are primary neural stem that give rise to oligodendrocytes, astrocytes, and olfactory bulb (OB) GABAergic interneurons in late embryogenesis. There fundamental gaps understanding how these diverse cell subtypes generated. Here, by combining single-cell RNA-Seq with intersectional lineage analyses, we show beginning at around E16.5, neocortical RGCs start generate ASCL1 + EGFR apical multipotent intermediate progenitors (MIPCs), which then...
Neural stem cells (NSCs) in the prenatal neocortex progressively generate different subtypes of glutamatergic projection neurons. Following that, NSCs have a major switch their progenitor properties and produce γ-aminobutyric acid (GABAergic) interneurons for olfactory bulb (OB), cortical oligodendrocytes, astrocytes. Herein, we provide evidence molecular mechanism that underlies this state neocortical NSCs. We show at around E16.5, mouse start to GSX2-expressing (GSX2+) intermediate (IPCs)....
Neural stem cells in the postnatal telencephalic ventricular-subventricular zone (V-SVZ) generate new interneurons, which migrate tangentially through rostral migratory stream (RMS) into olfactory bulb (OB). The Sp8 and Sp9 transcription factors are expressed neuroblasts, as well immature mature interneurons V-SVZ-RMS-OB system. Here we show that coordinately regulate OB interneuron development: although null mutants no major defect, conditional deletion of both resulted a much more severe...
ABSTRACT Dopamine receptor DRD1-expressing medium spiny neurons (D1 MSNs) and dopamine DRD2-expressing (D2 are the principal projection in striatum, which is divided into dorsal striatum (caudate nucleus putamen) ventral (nucleus accumbens olfactory tubercle). Progenitors of these arise lateral ganglionic eminence (LGE). Using conditional deletion, we show that mice lacking transcription factor genes Sp8 Sp9 lose virtually all D2 MSNs as a result reduced neurogenesis LGE, whereas D1 largely...
The striatum is a central regulator of behavior and motor function through the actions D1 D2 medium-sized spiny neurons (MSNs), which arise from common lateral ganglionic eminence (LGE) progenitor. molecular mechanisms cell fate specification these two neuronal subtypes are incompletely understood. Here, we found that deletion murine Meis2, highly expressed in LGE derivatives, led to large reduction striatal MSNs due block their differentiation. Meis2 directly binds Zfp503 Six3 promoters...
Dogs are pivotal in Echinococcus granulosus transmission to humans, and dog vaccination provides a very practical cost-effective prevention strategy. We vaccinated dogs with soluble native proteins isolated from protoscoleces of E. induced significant suppression worm growth egg production. Accordingly, we tested for vaccine efficacy using recombinant derived developmentally regulated gene family (egM) specifically expressed mature adult worms. Three egM genes--egM4, egM9, egM123--were...
MicroRNAs (miRNAs) are important post-transcriptional regulators which control growth and development in eukaryotes. The cestode Echinococcus granulosus has a complex life-cycle involving different stages but the mechanisms underpinning this development, including involvement of miRNAs, remain unknown. Using Illumina next generation sequencing technology, we sequenced at genome-wide level three small RNA populations from adult, protoscolex cyst membrane E. granulosus. A total 94 pre-miRNA...
In adult rodent and monkey brains, newly born neurons in the subventricular zone (SVZ) wall of lateral ventricle migrate into olfactory bulb (OB) via rostral migratory stream (RMS). A recent study reported that interneurons are constantly generating human striatum from SVZ. contrast, by taking advantage continuous expression Sp8 neuroblast stage through differentiation mature interneurons, we found SVZ does not generate new for striatum. RMS, very few neuroblasts were observed, most them...
Abstract The seat of human intelligence is the cerebral cortex, which responsible for our exceptional cognitive abilities. Identifying principles that lead to development large-sized cortex will shed light on what makes brain and species so special. remarkable increase in number cortical pyramidal neurons size mainly because radial glial cells, primary neural stem cells generate more than 130 days, whereas same process takes only about 7 days mice. molecular mechanisms underlying this...
Cortical radial glia (RGs) sequentially generate pyramidal neurons (PyNs) and glia. In this study, we investigated the molecular logic underlying cortical cellular diversification using time-series scRNA-seq snATAC-seq on FlashTag- or hGFAP-GFP-labeled mouse progenitors across embryonic postnatal stages. Our data revealed that RGs transition from early to late over time, producing intermediate neuronal (INPs) glial (IGPs). While INPs expand exclusively PyNs, IGPs progress young old,...
During cortical development, radial glial cells (neural stem cells) initially are neurogenic, generating intermediate progenitor that exclusively produce glutamatergic pyramidal neurons. Next, generate tripotential (Tri-IPCs) give rise to astrocytes and oligodendrocytes, olfactory bulb interneurons. The molecular mechanisms underlying the transition from neurogenesis gliogenesis, subsequent fate determination of astrocytes, interneurons, remain unclear. Here, we report extracellular...
Background Antigen B (EgAgB) is a major protein produced by the metacestode cyst of Echinococcus granulosus, causative agent cystic hydatid disease. This has been shown to play an important role in modulating host immune responses, although its precise biological function still remains unknown. It generally accepted that EgAgB comprised gene family five subfamilies which are highly polymorphic, but actual number genes present Methodology/Principal Findings Based on published sequences for...
Immature neurons generated by the subpallial MGE tangentially migrate to cortex where they become parvalbumin-expressing (PV+) and somatostatin (SST+) interneurons. Here, we show that Sp9 transcription factor controls development of MGE-derived cortical SP9 is expressed in subventricular zone migrating null conditional mutant mice have approximately 50% reduction interneurons, an ectopic aggregation embryonic ventral telencephalon, increased ratio SST+/PV+ RNA-Seq ChIP-Seq reveal regulates...
Generation of olfactory bulb (OB) interneurons requires neural stem/progenitor cell specification, proliferation, differentiation, and young interneuron migration maturation. Here, we show that the homeobox transcription factors Dlx1/2 are central essential components in transcriptional code for generating OB interneurons. In constitutive null mutants, differentiation GSX2+ ASCL1+ cells dorsal lateral ganglionic eminence is blocked, resulting a failure generation. conditional mutants...
Neural stem cells in the subventricular zone (SVZ) of lateral ventricle generate new interneurons, which migrate tangentially through rostral migratory stream (RMS) to olfactory bulb (OB). The PROK2 (prokineticin 2) and PROKR2 receptor signaling pathway has been identified cause human Kallmann syndrome, a developmental disease that associates hypogonadism with anosmia (OB defects). However, identities properties PROK2+ PROKR2+ SVZ-RMS-OB remain largely unknown. Here we examine expression...
Abstract The striatum is structurally highly diverse, and its organ functionality critically depends on normal embryonic development. Although several studies have been conducted the gene functional changes that occur during striatal development, a system-wide analysis of underlying molecular lacking. Here, we present comprehensive transcriptome profile allows us to explore trajectory development identify correlation between Huntington’s disease (HD). Furthermore, applied an integrative...
The generation and differentiation of cortical projection neurons are extensively regulated by interactive programs transcriptional factors. Here, we report the cooperative functions transcription factors Bcl11a Bcl11b in regulating development neurons. Among cells derived from neural stem cells, is expressed progenitors neurons, while expression restricted to Using conditional knockout mice, show that deficiency leads reduced proliferation precocious progenitor which exacerbated when...
Abstract The temporal lobe of the human brain contains entorhinal cortex (EC). This region is a highly interconnected integrative hub for sensory and spatial information; it also has key role in episodic memory formation main source cortical hippocampal inputs 1–4 . EC continues to develop during childhood 5 , but neurogenesis neuronal migration are widely considered be complete by birth. Here we show that many young neurons migrating into postnatal adjacent regions, with large tangential...