A5089510225- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Biology Techniques and Applications
- Spinal Cord Injury Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Caveolin-1 and cellular processes
- RNA modifications and cancer
- Nerve Injury and Rehabilitation
- MicroRNA in disease regulation
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- Atmospheric Ozone and Climate
- Epigenetics and DNA Methylation
- Memory and Neural Mechanisms
- Tissue Engineering and Regenerative Medicine
- Collagen: Extraction and Characterization
- Cellular Mechanics and Interactions
- Atmospheric chemistry and aerosols
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
University of California, San Francisco
2019-2025
Neurological Surgery
2019-2025
Broad Center
2019-2025
Broad Institute
2021-2025
Universidade Federal de Juiz de Fora
2023
Universidade Federal do Rio de Janeiro
2010-2023
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2020
Institute of Biophysics
2018
University of Münster
2016-2018
The adult mouse brain contains an extensive neurogenic niche in the lateral walls of ventricles. This epithelium, which has a unique pinwheel organization, multiciliated ependymal (E1) cells and neural stem (B1). postnatal germinal epithelium develops from embryonic ventricular zone, but lineage relationship between E1 B1 remains unknown. Distinct subpopulations radial glia (RG) late early development either expand their apical domain >11-fold to form or retain small domains that coalesce...
The ventricular-subventricular zone (V-SVZ), on the walls of lateral ventricles, harbors largest neurogenic niche in adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) different locations within V-SVZ produce subtypes new neurons for olfactory bulb. molecular signatures underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset revealing two populations NSPCs reside non-overlapping domains either dorsal...
Cell therapy is a promising strategy to pursue the unmet need for treatment of spinal cord injury (SCI). Although several studies have shown that adult mesenchymal cells contribute improve outcomes SCI, descripton pro-regenerative events triggered by these still lacking. Here we investigated regenerative properties human adipose tissue derived stromal (hADSCs) in rat model compression. Cells were delivered directly into parenchyma immediately after injury. Human ADSCs promoted functional...
Fractones are extracellular matrix structures in the neural stem cell niche of subventricular zone (SVZ), where they appear as round deposits named bulbs or thin branching lines called stems. Their cellular origin and what determines their localization at this site is poorly studied, it remains unclear whether influence progenitor formation, proliferation, and/or maintenance. To address these questions, we analyzed whole-mount preparations lateral ventricle male female mice by confocal...
Abstract Angiogenesis is considered to mediate the beneficial effects of mesenchymal cell therapy in spinal cord injury. After a moderate balloon-compression injury rats, injections either human adipose tissue-derived stromal/stem cells (hADSCs) or their conditioned culture media (CM-hADSC) elicited angiogenesis around lesion site. Both therapies increased vascular density, but presence hADSCs tissue was required for full maturation new blood vessels. Only animals that received hADSC...
Abstract The temporal lobe of the human brain contains entorhinal cortex (EC). This region is a highly interconnected integrative hub for sensory and spatial information; it also has key role in episodic memory formation main source cortical hippocampal inputs 1–4 . EC continues to develop during childhood 5 , but neurogenesis neuronal migration are widely considered be complete by birth. Here we show that many young neurons migrating into postnatal adjacent regions, with large tangential...
Regeneration of spinal cord injury (SCI) is a major topic biomedical research. Laminin an extracellular matrix protein implicated in neural development and regeneration, but despite that, there are no reports exogenous laminin contributing to improve the outcome experimental SCI. Here we investigated whether biomimetic polymer assembled on pH acidification, henceforth called polylaminin, could be used treat SCI rats. Acute local injection not nonpolymerized laminin, improved motor function...
Neurogenesis and gliogenesis continue in the Ventricular-Subventricular Zone (V-SVZ) of adult rodent brain. B1 cells are astroglial derived from radial glia that function as primary progenitors or neural stem (NSCs) V-SVZ. cells, which have a small apical contact with ventricle, decline numbers during early postnatal life, yet neurogenesis continues into adulthood. Here we found second population V-SVZ (B2 cells), do not NSCs B2 cell increase postnatally, remain constant 12-month-old mice...
Unlike the central nervous system, peripheral nerves can regenerate after injury. However, depending on size of lesion, endogenous regenerative potential is not enough to replace lost nerve tissue. Many strategies have been used generate biomaterials capable restoring functions. Here, we set out investigate whether adsorbing extracellular matrix protein, laminin (LM), poly-ℇ-caprolactone (PCL) filaments would enhance functional regeneration. Initial in vitro studies showed that explants...
ABSTRACT Spinal cord injury (SCI) is still a major challenge for translational medicine. Polylaminin stabilized form of laminin, natural multifunctional protein, previously shown to promote neuroprotection and axonal growth after SCI in rodents. Here we present the results confirmatory preclinical study performed rats first human trial polylaminin treat acute SCI. The latter was an open-label, single-armed academic designed include only patients diagnosed with functional complete SCI,...
The entorhinal cortex (EC) is a highly-interconnected hub for multisensory integration and memory processing 1–3 , containing diverse neuronal subtypes 4,5 including subpopulations that are uniquely spatially-tuned 6,7 . Although many spatial functions develop in infancy, it considered neurogenesis migration to the EC occurs prenatally. Here we show postnatal human temporal lobe contains prominent stream with large chains of young migrating neurons individual breaking away directed into EC....
Abstract Fractones are extracellular matrix structures in the neural stem cell niche of subventricular zone ( SVZ ), where they appear as round deposits named bulbs or thin branching lines called stems. Their cellular origin and what determines their localization at this site is poorly studied it remains unclear whether influence progenitor cells formation, proliferation and/or maintenance. To address these questions, we analyzed whole mount preparations lateral ventricle by confocal...
Abstract Understanding the atmospheric sulfur cycle is fundamental in developing actions to mitigate its effects on acid rain formation. Solvation of HOSO and HSO 2 species has been indicated as a possible pathway To observe differences behavior these isomers water medium, electrical reactivity properties - electronic chemical potential (μ), hardness (η), electrophilicity (ϵ)- have obtained. Once respective first solvation shells ( 15H O for 23 H ) are considered, an increase 25 % dipole...
This is a protocol to save your experiment and successfully demultiplex nuclei using CellPlex, the cholesterol-modified-oligo (CMO) barcodes from 10x Genomics on snap-frozen tissue. Generally speaking, tissue perform really poorly with CMOs. crucial information was not in their initial documentation when product launched, leading costly losses. However, if you are multiplexing samples different human donors, there way of saving data still demultiplexing quickly reliably. By taking advantage...
Protocol to isolate nuclei from snap-frozen human brain samples for sn-RNAseq
Protocol to isolate nuclei from snap-frozen human brain samples for sn-RNAseq
Abstract The ventricular-subventricular zone (V-SVZ) is home to the largest neurogenic niche in adult mouse brain. Previous work has demonstrated that resident stem cells different locations within V-SVZ produce subtypes of new neurons for olfactory bulb. While great progress been made understanding differences regional cell potential using viral and genetic lineage tracing strategies, core molecular heterogeneity underlies these largely unknown. Here we present single whole-cell nucleus...