A5100661529- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Microplastics and Plastic Pollution
- Antimicrobial Peptides and Activities
- Microbial Natural Products and Biosynthesis
- biodegradable polymer synthesis and properties
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Microbial bioremediation and biosurfactants
- Marine Biology and Environmental Chemistry
- Photoreceptor and optogenetics research
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Seaweed-derived Bioactive Compounds
- Phytase and its Applications
- Echinoderm biology and ecology
- Chromium effects and bioremediation
- Cancer Research and Treatments
- Mine drainage and remediation techniques
- Mercury impact and mitigation studies
Institute of Oceanology
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2010-2025
Emory University
2025
Qingdao National Laboratory for Marine Science and Technology
2016-2024
Academia Sinica
2021-2024
Institute of Oceanology
2023-2024
Laoshan Laboratory
2023
National Marine Life Center
2021
South China Sea Institute Of Oceanology
2020
Plastic wastes are becoming the most common form of marine debris and present a growing global pollution problem. Here, we used screening approach on hundreds plastic waste-associated samples discovered bacterial community capable efficiently colonizing degrading both poly(ethylene terephthalate) (PET) polyethylene (PE). Using absolute quantitative 16S rRNA sequencing cultivation methods, obtained corresponding abundance purified cultures three strains that mediated degradation. We further...
Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult control. Bacillus species have been extensively explored for biological control of many fungal diseases. In present study, marine bacterium subtilis BS155 showed strong antifungal activity against M. The active metabolites were isolated identified as cyclic lipopeptides (CLPs) fengycin family, named BS155, combination high-performance liquid chromatography (HPLC)...
Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors, thus leading to major problems in many fields, such as clinical infection, food contamination, marine biofouling. In this study, we report the purification characterization of an exopolysaccharide EPS273 from culture supernatant P. stutzeri 273. The not only effectively inhibits biofilm formation but also disperses preformed PAO1. High performance liquid chromatography traces...
Sorafenib is a multikinase inhibitor that effective in treating advanced liver cancer. Although its mechanism of action through several established cancer-related protein kinase targets well-characterized, sorafenib induces variable responses among human tumors, and the cause for this variation yet unknown. To investigate underlying mechanisms, we applied mass spectrometry-based proteomic analysis to Huh7.5 cancer cells found significantly affected expression key lipogenic enzymes,...
Zero-valent sulfur (ZVS) has been shown to be a major intermediate in the deep-sea cold seep of South China Sea based on our previous work, however, microbial contribution formation ZVS remained unclear. Here, we describe novel thiosulfate oxidation pathway discovered bacterium Erythrobacter flavus 21-3, which provides new clue about ZVS. Electronic microscopy, energy-dispersive, and Raman spectra were used confirm that E. 21-3 effectively converts We next combined proteomic genetic method...
Protein fate in higher eukaryotes is controlled by three complexes that share conserved architectural elements: the proteasome, COP9 signalosome, and eukaryotic translation initiation factor 3 (eIF3). Here we reconstitute 13-subunit human eIF3 Escherichia coli , revealing its structural core to be eight subunits with orthologues proteasome lid complex signalosome. This binds small (40S) ribosomal subunit, factors involved mRNA cap-dependent initiation, hepatitis C viral (HCV) internal...
The initiation of protein synthesis plays an essential regulatory role in human biology. At the center pathway, 13-subunit eukaryotic translation factor 3 (eIF3) controls access other factors and mRNA to ribosome by unknown mechanisms. Using electron microscopy (EM), bioinformatics biochemical experiments, we identify two highly conserved RNA-binding motifs eIF3 that direct from hepatitis C virus internal entry site (HCV IRES) RNA. Mutations motif subunit eIF3a weaken binding HCV IRES 40S...
This study was initiated to screen for marine bacterial agents biocontrol Magnaporthe grisea, a serious fungal pathogen of cereal crops. A strain, isolated from the cold seep in deep sea, exhibited strong growth inhibition against M. and strain identified designated as Bacillus sp. CS30. The corresponding antifungal were purified by acidic precipitation, sequential methanol extraction, Sephadex LH-20 chromatography, reversed phase high-performance liquid chromatography (RP-HPLC), two peaks...
Abstract Candidatus Izemoplasma, an intermediate in the reductive evolution from Firmicutes to Mollicutes, was proposed represent a novel class of free-living wall-less bacteria within phylum Tenericutes. Unfortunately, paucity pure cultures has limited further insights into their physiological and metabolic features as well ecological roles. Here, we report first successful isolation Izemoplasma representative deep-sea methane seep, strain zrk13, using DNA degradation-driven method given...
Plastic wastes have been recognized as the most common and durable marine contaminants, which are not only found in shallow water, but also on sea floor. However, whether deep-sea microorganisms evolved capability of degrading plastic remains elusive. In this study, a bacterium Bacillus velezensis GUIA was to be capable waterborne polyurethane. Transcriptomic analysis showed that supplement polyurethane upregulated expression many genes related spore germination, indicating presence had...
Abstract Many polysaccharides isolated from plants have exhibited promising antitumor activities. The aim of this study is to investigate the activity novel polysaccharide named SPS Sargassum integerrimum , elucidate underlying anticancer mechanism in a human lung cancer cell line A549 and evaluate its anti-angiogenic both vitro vivo . results show that significantly reduces cells viability dose- time-dependent manner via MTT method. Flow cytometry analysis indicates could induce apoptosis,...
Phenazine and its derivatives are very important secondary metabolites produced from Pseudomonas spp. have exhibited broad-spectrum antifungal antibacterial activities. However, till date, there few reports about marine derived production of phenazine metabolites. In this study, we isolated a aeruginosa strain PA31x which natural product inhibiting the growth Vibrio anguillarum C312, one most serious bacterial pathogens in aquaculture. Combining high-resolution electro-spray-ionization mass...
Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, genetic and physiological adaptations bacteria stress still unclear. Here, we show that marine bacterium Pseudomonas stutzeri 273 is resistant 50 μM Hg2+ removes up 94% from culture. Using gene homologous recombination complementation, genes encoding Hg2+-transport proteins MerT, MerP, mercuric reductase MerA regulatory protein...
Calcium carbonate (CaCO3) biomineralization has been investigated due to its wide range of scientific and technological implications, however, the molecular mechanisms this important geomicrobiological process are largely unknown. Here, a urease-positive marine actinobacterium Brevibacterium lines BS258 was demonstrated effectively form CaCO3 precipitates. Surprisingly, bacterium could also dissolve formed with increase Ca2+ concentration. To disclose biomineralization, genome B. further...
The increasing emergence of multidrug-resistant pathogens and development biopreservatives in food industries has increased the demand novel safe antimicrobial agents. In this study, a marine bacterial strain Bacillus licheniformis M1 was isolated exhibited obvious activities against foodborne pathogens, especially methicillin-resistant Staphylococcus aureus. agent purified identified as peptide, which designated bacipeptin, corresponding mechanism further investigated by electron microscopy...
Bacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. Here, we isolated marine bacterium (Vibrio alginolyticus strain 178) from seamount in tropical West Pacific that exhibits vigorous on agar plates severe pathogenicity to zebrafish. We found V. 178 was significantly suppressed by another bacterium, Bacillus sp. 176, same niche. isolated, purified, characterized two different cyclic...
Abstract A multifunctional enzyme is one that performs multiple physiological functions, thus benefiting the organism. Characterization of enzymes important for researchers to understand how organisms adapt different environmental challenges. In present study, we report discovery a novel Amy63 produced by marine bacterium Vibrio alginolyticus 63. Remarkably, possesses amylase, agarase and carrageenase activities. substrate promiscuous α-amylase, with priority order starch, carrageenan agar....