A5101606855- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant biochemistry and biosynthesis
- Chromium effects and bioremediation
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Phytochemical compounds biological activities
- Advanced Photocatalysis Techniques
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Tea Polyphenols and Effects
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Toxoplasma gondii Research Studies
- Fermentation and Sensory Analysis
- Reproductive tract infections research
- Microbial Community Ecology and Physiology
- 3D Printing in Biomedical Research
Chinese Academy of Sciences
2012-2024
Jiangxi Normal University
2019-2024
Jiangxi Science and Technology Normal University
2023-2024
Guangxi Institute of Botany
2012-2024
South China Sea Institute Of Oceanology
2012-2019
Institute of Oceanology
2012-2019
University of Chinese Academy of Sciences
2012
Huazhong Agricultural University
2009
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded biosynthetic gene clusters. Information about these clusters, is currently dispersed throughout the literature, making it difficult exploit. To facilitate consistent systematic deposition retrieval data on we propose Minimum a Biosynthetic Gene cluster (MIBiG) standard.
Tuberculosis remains one of the world's deadliest communicable diseases, novel anti-tuberculosis agents are urgently needed due to severe drug resistance and co-epidemic tuberculosis/human immunodeficiency virus. Here, we show isolation six anti-mycobacterial ilamycin congeners (1-6) bearing rare L-3-nitro-tyrosine L-2-amino-4-hexenoic acid structural units from deep sea-derived Streptomyces atratus SCSIO ZH16. The biosynthesis L-3-nitrotyrosine as well three pre-tailoring two post-tailoring...
Abstract Griseoviridin (GV) and viridogrisein (VG, also referred to as etamycin), produced by Streptomyces griseoviridis , are two chemically unrelated compounds belonging the streptogramin family. Both of these natural products demonstrate broad‐spectrum antibacterial activity constitute excellent candidates for future drug development. To elucidate biosynthetic machinery associated with production unique antibiotics, gene cluster responsible both GV VG was identified within genome...
Bioactive secondary metabolites from Streptomycetes are important sources of lead compounds in current drug development. Streptomyces costaricanus SCSIO ZS0073, a mangrove-derived actinomycete, produces actinomycin D, clinically used therapeutic for Wilm's tumor the kidney, trophoblastic tumors and rhabdomyosarcoma. In this work, we identified biosynthetic gene cluster (BGC) acn by detailed analyses S. ZS0073 genome. This organism D with titer ~69.8 μg mL-1 along traces Xoβ. The localized to...
Passiflora edulis Sims (passion fruit) seeds are often discarded as byproducts during juice processing. In fact, the of considerable commercial value in food and cosmetics industry because their rich polyphenols, especially piceatannol. this study, high-speed countercurrent chromatography (HSCCC) was applied for separation stilbene polyphenols from passion fruit seeds. The n-hexane-ethyl acetate-methanol-water (1:2:1:2.8, v/v) found to be optimum two-phase solvent preparation two major...
The biosynthetic gene cluster governing production of anti-infective marformycins was identified from deep sea-derived Streptomyces drozdowiczii SCSIO 10141. putative mfn (45 kb, 20 orfs) found to encode six NRPSs and related proteins for cyclodepsipeptide core construction (mfnCDEFKL), a methionyl-tRNA formyltransferase (mfnA), SAM-dependent methyltransferase (mfnG), cytochrome P450 monooxygenase piperazic acid moiety hydroxylation (mfnN); notably, only MfnN uses an intact intermediate as...
The chemical examination of the solid cultures deep-sea-derived fungus Penicillium chrysogenum MCCC 3A00292 resulted in isolation three new versiol-type analogues, namely peniciversiols A–C (1–3), and two novel lactone derivatives, penicilactones A B (6 7), along with 11 known polyketides. planar structures compounds were determined by comprehensive analyses high-resolution electrospray ionization mass spectroscopy (HRESIMS) nuclear magnetic resonance (NMR) data, while their absolute...
Himastatin is a novel antibiotic featuring bicyclohexadepsipeptide structure. On the himastatin biosynthesis pathway, three cytochrome P450s (HmtT, HmtN, HmtS) are responsible for post‐tailoring of cyclohexadepsipeptide backbone. Here we report crystal structures HmtT and HmtN. The overall these two proteins homologous to other P450s. However, exceptionally long F–G loop in has highly unusual conformation extends deep into active site. As result, F/G helices both kinked. In contrast, HmtN...
Abstract Griseoviridin (GV) is an A‐type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in GV/VG gene cluster. To explore role of pathway, we inactivated sgvP gene. The resulting Δ mutant generated two new products: GV‐1 GV‐2, both lacking CS bridge. In trans complementation into strain partially restores GV production. Feeding [1‐ 13 C]‐labeled cysteine to...
The CO2 photocatalytic conversion efficiency of the semiconductor photocatalyst is always inhibited by sluggish charge transfer and undesirable affinity. In this work, we prepare a series K-doped In2O3 catalysts with concomitant oxygen vacancies (OV) via hydrothermal method, followed low-temperature sintering treatment. Owing to synergistic effect K doping OV, separation affinity are synchronously promoted. Particularly, when P/P0 = 0.010, at room temperature, adsorption capacity optimal...
Griseoviridin (GV) and viridogrisein (VG, also referred as etamycin), both biosynthesized by a distinct 105 kb biosynthetic gene cluster (BGC) in Streptomyces griseoviridis NRRL 2427, are pair of synergistic streptogramin antibiotics very important treating infections many multi-drug resistant microorganisms. Three transporter genes, sgvT1-T3 have been discovered within the GV/VG BGC, but function these efflux transporters not identified.In present study, we identified different roles three...
Two Pseudoalteromonas strains, SCSIO 04301 and 11900, were isolated from the South China Sea, both strains form biofilms. Here we present draft genome sequences of these two which will aid study marine microbes that are adapted to sediments or associated with eukaryotic hosts.
Three atypical regulatory genes, hmtABD have been discovered within the himastatin biosynthetic gene cluster (BGC) in Streptomyces hygroscopicus ATCC 53653 and roles of their products identified. HmtA HmtD do not show any structurally distinct features characteristic function yet were shown to play important repressive stimulatory roles, respectively, related biosynthesis. HmtB encodes a conserved acetylglutamate kinase; new member this family serves as repressor secondary metabolism....
Abstract Background Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown contain an intact but caerulomycin A (CRM A) BGC- cam its genome. Thus, genome mining work preformed activate strain’s production CRM A, immunosuppressive drug lead with diverse bioactivities. Results To well expression , ribosome engineering...
Two mutases, MtdL and Hyg20, are reported. Both able to functionally drive the biosynthesis of GDP-α-l-fucofuranose. enzymes catalyze similar functions, catalytically enabling bidirectional reaction between GDP-β-l-fucopyranose GDP-α-l-fucofuranose using only divalent cations as cofactors. This realization is but one a number important insights into fucofuranose presented herein.