A5074705372- Hemoglobinopathies and Related Disorders
- Fungal and yeast genetics research
- Iron Metabolism and Disorders
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Erythrocyte Function and Pathophysiology
- Chronic Myeloid Leukemia Treatments
- Antibiotic Resistance in Bacteria
- Antifungal resistance and susceptibility
- Prenatal Screening and Diagnostics
- Animal Nutrition and Physiology
- Glutathione Transferases and Polymorphisms
- Redox biology and oxidative stress
- Chronic Lymphocytic Leukemia Research
- Bacterial biofilms and quorum sensing
- Acute Myeloid Leukemia Research
- Kruppel-like factors research
- RNA modifications and cancer
- Eosinophilic Disorders and Syndromes
- Bone and Joint Diseases
- Gut microbiota and health
- Heat shock proteins research
- Amphibian and Reptile Biology
- Folate and B Vitamins Research
- Plant-Microbe Interactions and Immunity
Universidade Federal de São Carlos
2015-2024
Universidade Estadual de Campinas (UNICAMP)
2002-2024
Universidade Federal do Rio de Janeiro
2024
D’Or Institute for Research and Education
2024
DNA Consult Genética e Biotecnologia (Brazil)
2024
Universidade de São Paulo
2017-2024
Universidade Federal de Pernambuco
2023
Massachusetts General Hospital
2017
Harvard University
2017
Harvard–MIT Division of Health Sciences and Technology
2017
Klebsiella pneumoniae is an important opportunistic pathogen that commonly causes nosocomial infections and contributes to substantial morbidity mortality. We sought investigate the antibiotic resistance profile, pathogenic potential clonal relationships between K. (n = 25) isolated from patients sources at a tertiary care hospital's intensive units (ICUs) in northern region of Brazil. Most isolates 21, 84%) were classified as multidrug resistant (MDR) with high-level β-lactams,...
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived agricultural feedstocks by yeast Saccharomyces cerevisiae . One most widely adopted strains PE-2, heterothallic diploid naturally adapted to sugar cane process used in Brazil. Here we report molecular genetic analysis PE-2 (JAY270), and complete genome sequence haploid derivative (JAY291). The JAY270 highly heterozygous (∼2 SNPs/kb) has several structural polymorphisms between homologous chromosomes. These...
Abstract Background The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao ( Theobroma ). It a hemibiotrophic pathogen that colonizes apoplast cacao's meristematic tissues as biotrophic pathogen, switching to saprotrophic lifestyle during later stages infection. M. , together with related species roreri are pathogens aerial parts plant, an uncommon characteristic order Agaricales. A genome survey (1.9× coverage) was analyzed evaluate...
Abstract The Cell Wall Integrity (CWI) pathway is the primary signaling cascade that controls de novo synthesis of fungal cell wall, and in Saccharomyces cerevisiae this event highly dependent on RLM1 transcription factor. Here, we investigated function RlmA pathogen Aspergillus fumigatus. We show ΔrlmA strain exhibits an altered wall organization addition to defects related vegetative growth tolerance wall-perturbing agents. A genetic analysis indicated rlmA positioned downstream pkcA mpkA...
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). The retracted at the request of Founding Editor, Prof. Lawrence H. Lash, basis that there is clear evidence findings are unreliable: https://publicationethics.org/files/retraction-guidelines-cope.pdf. Additional external review this published paper raised several concerns. While a properly conducted clinical trial certainly welcome,...
Aspergillus fumigatus is an opportunistic human pathogen, which causes the life-threatening disease, invasive pulmonary aspergillosis. In fungi, cell wall homeostasis controlled by conserved Cell Wall Integrity (CWI) pathway. A. this signaling cascade partially characterized, but mechanisms it activated are not fully elucidated. study we investigated role of protein kinase C (PkcA) in cascade. Our results suggest that pkcA essential gene and response to stress. Subsequently, constructed...
Standing among the front defense strategies against pathogens, host phagocytic cells release various oxidants. Therefore, pathogens have to cope with stressful conditions at site of infection. Peroxiredoxins (Prx) are highly reactive and abundant peroxidases that can support virulence persistence in distinct hosts. Here, we revealed opportunistic human pathogen A. fumigatus presents three 1-Cys Prx (Prx6 subfamily), which is unprecedented. We showed PrxB PrxC were mitochondria, while Prx1...
Chryseobacterium indologenes is a non-glucose-fermenting Gram-negative bacillus. This emerging multidrug resistant opportunistic nosocomial pathogen can cause severe infections in neonates and immunocompromised patients. study aimed to present the first detailed draft genome sequence of multidrug-resistant C. strain isolated from cerebrospinal fluid an infant hospitalized at Neonatal Intensive Care Unit Brazilian Tertiary Hospital. We analyzed susceptibility different antibiotics using VITEK...
Serratia marcescens has emerged as an important opportunistic pathogen responsible for nosocomial and severe infections. Here, we determined phenotypic molecular characteristics of 54 S. isolates obtained from patient samples intensive-care-unit (ICU) neonatal (NIUC) a Brazilian tertiary hospital. All were resistant to beta-lactam group antibiotics, 92.6% (50/54) not susceptible tigecycline. Furthermore, 96.3% showed intrinsic resistance polymyxin E (colistin), last-resort antibiotic the...
The use of thermotolerant yeast strains can improve the efficiency ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification (SSF) starch or lignocellulosic biomass.We identified characterized physiology a new strain (LBGA-01) able ferment °C, which is more resistant stressors as sucrose, furfural CAT-1 industrial strain. Furthermore, this showed similar...
Sickle cell anemia (SCA) presents a complex interplay of factors, with the production high levels reactive oxygen species (ROS) and chronic inflammatory process leading to oxidative stress. In this context, efficient action antioxidant systems becomes crucial, particular emphasis on peroxiredoxins (PRDXs) due their abundance vital roles. Our primary objective was establish associations between gene protein expression PRDXs 1, 2, 6, as well reducers TRX1, TRXR1, SRX1, characteristic...
Salmonellosis is a poultry industry and public health concern worldwide. Recently, Salmonella enterica serovar Heidelberg (SH) has been reported in broilers Brazil. The effect of feeding blend three strains Bacillus subtilis (PRO) was studied orally challenged (107 CFU/chick) or not with SH isolated south Brazil (UFPR1 strain). Twelve male Cobb 500 per pen were randomly assigned to 6 treatments 3x2 factorial experiment where PRO added at 0, 250 500g/ton broiler feed fed either SH-challenged...
The deleterious effects of human-induced climate change have long been predicted. However, the imminent emergence and spread new diseases, including fungal infections through rise thermotolerant strains, is still neglected, despite being a potential consequence global warming. Thermotolerance remarkable virulence attribute mold Aspergillus fumigatus . Under high-temperature stress, opportunistic pathogens deploy an adaptive mechanism known as heat shock (HS) response controlled by...
Abstract α‐Hemoglobin stabilizing protein (AHSP) is a potential modifier of β‐thalassemia by virtue its ability to detoxify excess free α‐globin. However, examination patients with from few geographic regions failed identify obvious AHSP mutations. We extended these studies analyzing gene sequences in 366 anonymous individuals five different areas the world. detected numerous polymorphisms comprising 18 haplotypes and two rare missense Two sequence variations produce functional effects...
Objective The aim of this study was to verify how a pair monozygotic twins would respond light-emitting diode therapy (LEDT) or placebo combined with strength-training program during 12 weeks. Design This case-control enrolled male twins, allocated randomly LEDT therapies. Light-emitting applied from flexible array (λ = 850 nm, total energy 75 J, t 15 seconds) both quadriceps femoris muscles each twin immediately after strength training session (3 times/wk for weeks) consisting leg press and...
A remarkable feature of the human pathogen Aspergillus fumigatus is its ability to produce impressive amounts infectious propagules known as conidia. These particles reach immunocompromised patients and may initiate a life-threatening mycosis. The conidiation process in governed by sequence proteins that coordinate development conidiophores. This requires remodeling cell wall so conidiophores can rise withstand chains events regulating during are currently unknown. Here, we show integrity...
The initiation of Aspergillus fumigatus infection occurs via dormant conidia deposition into the airways. Therefore, conidial germination and subsequent hyphal extension growth occur in a sustained heat shock (HS) environment promoted by host. cell wall integrity pathway (CWIP) essential eukaryotic chaperone Hsp90 are critical for fungi to survive HS. Although A. is thermophilic fungus, mechanisms underpinning HS response not thoroughly described important define its role pathogenesis,...
Thermotolerance is a remarkable virulence attribute of Aspergillus fumigatus, but the consequences heat shock (HS) to cell membrane this fungus are unknown, although structure one first detect changes in ambient temperature that imposes on prompt adaptative response. Under high-temperature stress, fungi trigger HS response controlled by transcription factors, such as HsfA, which regulates expression proteins. In yeast, smaller amounts phospholipids with unsaturated fatty acid (FA) chains...
β-thalassemia is a worldwide distributed monogenic red cell disorder, characterized by an absent or reduced beta globin chain synthesis. The unbalance of alpha-gamma and the presence pathological free iron promote severe oxidative damage, playing crucial role in erythrocyte hemolysis, exacerbating ineffective erythropoiesis decreasing lifespan blood cells (RBC). Catalase, glutathione peroxidase peroxiredoxins act together to protect RBCs from hydrogen peroxide insult. Among them, stand out...
The goal of this study was to develop conditional flocculant yeasts for use in the alcohol fermentation industry. Promoters were constructed completely repress gene transcription presence sugar and support strong expression after exhaustion compound. A fusion regulatory regions ADH2 promoter with FLO5 core regulate gene. This construct inserted into multicopy plasmids transformed laboratory strains Saccharomyces cerevisiae, whereby cells selected by sedimentation from bulk medium exhaustion,...