A5075393732- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Circular RNAs in diseases
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Retinoids in leukemia and cellular processes
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Neonatal Respiratory Health Research
- T-cell and Retrovirus Studies
- Reproductive System and Pregnancy
- Animal Disease Management and Epidemiology
- Chronic Myeloid Leukemia Treatments
- Cancer-related Molecular Pathways
- Glioma Diagnosis and Treatment
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
Universidade de São Paulo
2013-2024
Universidade de Ribeirão Preto
2008-2024
Fundação Hemopa
2016-2021
Clinics Hospital of Ribeirão Preto
2012-2021
Instituto Nacional de Tecnologia
2020
Universidade Federal de São Carlos
2001-2017
Massachusetts General Hospital
2017
Harvard University
2017
Harvard–MIT Division of Health Sciences and Technology
2017
Universidad San Carlos
2016
Mesenchymal stem cells (MSCs) give origin to the marrow stromal environment that supports hematopoiesis. These present a wide range of differentiation potentials and complex relationship with hematopoietic (HSCs) endothelial cells. In addition bone (BM), MSCs can be obtained from other sites in adult or fetus. We isolate umbilical cord (UC) veins are morphologically immunophenotpically similar BM. culture, these capable differentiating vitro into adipocytes, osteoblasts, condrocytes. The...
Mesenchymal stem cells (MSCs) are multipotent precursors present in adult bone marrow, that differentiate into osteoblasts, adipocytes and myoblasts, play important roles hematopoiesis. We examined gene expression of these by serial analysis expression, found collagen I, secreted protein acidic rich cysteine (osteonectin), transforming growth factor beta- (TGF-β) induced, cofilin, galectin-1, laminin-receptor 1, cyclophilin A, matrix metalloproteinase-2 among the most abundantly expressed...
Microarray studies have revealed the differential expression of several genes in mantle cell lymphoma (MCL), but it is unknown which these differences are dependent on transformed MCL itself or tumour microenvironment. To investigate and signalling pathways aberrantly expressed cells we used oligonucleotide microarrays to perform gene profiling both purified leukaemic their normal counterparts, naive B cells. A total 106 were differentially at least threefold compared with cells; 63...
Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system, due to an immune reaction against myelin proteins. Multipotent mesenchymal stromal cells (MSCs) present immunosuppressive effects and have been used for treatment diseases. In our study, gene expression profile in vitro immunomodulatory function tests were compare bone marrow-derived MSCs obtained from MS patients, at pre- postautologous hematopoietic stem cell transplantation (AHSCT) with...
Abstract Mesenchymal stem cells (MSCs) are known to induce the conversion of activated T into regulatory in vitro . The marker CD69 is a target canonical nuclear factor kappa‐B (NF‐κB) signalling and transiently expressed upon activation; however, stable expression defines with immunoregulatory properties. Given its enormous therapeutic potential, we explored molecular mechanisms underlying induction by MSCs. Peripheral blood CD3 + were cultured presence or absence CD4 cell mRNA was then...
Mesenchymal stromal cells (MSCs) are cultured that can give rise to mature mesenchymal under appropriate conditions and secrete a number of biologically relevant molecules may play an important role in regenerative medicine. Evidence indicates pericytes (PCs) correspond stem vivo MSCs when cultured, but comparison between the gene expression profiles PCs (cPCs) is lacking. We have devised novel methodology isolate from human adipose tissue compared cPCs obtained through traditional methods....
Recent evidence has shown that deregulated expression of members the micro RNA ‐29 (miR‐29) family may play a critical role in human cancer, including hematological malignancies. However, roles miR‐29 molecular pathophysiology T‐cell acute lymphoblastic leukemia (T‐ ALL ) not been investigated. Here, we show lower levels miR‐29a were significantly associated with higher blast counts bone marrow and increased disease‐free survival T‐ patients. Furthermore, are extremely reduced cells compared...
Abstract Regulatory T cells (Tregs) are essential regulators of immune tolerance. atRA and TGF-β can inhibit the polarization naïve into inflammatory Th17 cells, favoring generation stable iTregs, however regulatory mechanisms involved not fully understood. In this context, roles individual microRNAs in Tregs largely unexplored. Naïve were immunomagnetically isolated from umbilical cord blood activated with anti-human CD2/CD3/CD28 beads presence IL-2 alone (CD4 Med ) or addition TGF/atRA )....
Laryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be subtype of the head and neck (HNSCC). Despite significant advances in understanding treatment prognosis patients with LSCC has not improved recently. In present study, we sought understand better genetic mechanisms underlying development. Thirty-two tumor samples were collected from undergoing surgical resection LSCC. The submitted whole-genome cDNA microarray analysis aiming identify targets We also...
Promyelocytic leukemia-retinoic acid receptor alpha (PML-RARα) expression in acute promyelocytic leukemia (APL) impairs transforming growth factor beta (TGFβ) signaling, leading to cell advantage. Halofuginone (HF), a low-molecular-weight alkaloid that modulates TGFβ was used treat APL lines and non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice subjected transplantation with leukemic cells from human chorionic gonadotrophin-PML-RARα transgenic (TG). Cell cycle analysis...
Autologous haematopoietic stem-cell transplantation (AHSCT) has been experimented as a treatment in patients affected by severe forms of multiple sclerosis (MS) who failed to respond standard immunotherapy. The rationale AHSCT is 'reboot' the immune system and reconstitute new adaptive immunity. aim our study was identify, through robust unbiased transcriptomic analysis, any changes gene expression T-cells potentially underlying effect underwent non-myeloablative for MS. We evaluated...
Reprogramming of somatic cells to pluripotency promises boost cellular therapy. Most instances direct reprogramming have been achieved by forced expression defined exogenous factors using multiple viral vectors. The most used 4 transcription factors, octamer-binding factor (OCT4), (sex determining region Y)-box 2 (SOX2), Kruppel-like (KLF4), and v-myc myelocytomatosis oncogene homolog (C-MYC), can induce in mouse human fibroblasts. Here, we report that a new combination (T-cell...
Human mesenchymal stem cells (hMSCs) are adult multipotent that have high therapeutic potential due to their immunological properties. They can be isolated from several different tissues with bone marrow (BM) being the most common source. Because isolation procedure is invasive, other such as human umbilical cord vein (UCV) been considered. However, interchangeability remains unclear. In present study, total protein extracts of BM-hMSCs and UCV-hMSCs were quantitatively compared using...
Objective The aim of this study was to verify how a pair monozygotic twins would respond light-emitting diode therapy (LEDT) or placebo combined with strength-training program during 12 weeks. Design This case-control enrolled male twins, allocated randomly LEDT therapies. Light-emitting applied from flexible array (λ = 850 nm, total energy 75 J, t 15 seconds) both quadriceps femoris muscles each twin immediately after strength training session (3 times/wk for weeks) consisting leg press and...
Abstract Background Although promising for graft-versus-host disease (GvHD) treatment, MSC therapy still faces important challenges. For instance, increasing migratory capacity as well potentializing immune response suppression are of interest. GvHD management, preventing opportunistic infections is also a valuable strategy, since immunocompromised patients easy targets infections. LL-37 host defense peptide (HDP) that has been deeply investigated due to its immunomodulatory function. In...
Methods for the extraction of DNA from filamentous fungi are frequently laborious and time consuming because most available protocols include maceration in liquid nitrogen after mycelium has been grown a culture. This paper describes new method to replace those steps, which involves growth on cellophane disks overlaid solid medium use glass beads cell wall disruption. Extractions carried out by this provided approximately 2 μg total per disk fungus Trichoderma reesei. To assess DNA's...
Statins are widely used hypocholesterolemic drugs that block the mevalonate pathway, responsible for biosysnthesis of cholesterol. However, statins also have pleiotropic effects interfere with several signaling pathways. Mesenchymal stromal cells (MSC) a heterogeneous mixture can be isolated from variety tissues and identified by expression panel surface markers their ability to differentiate in vitro into osteocytes, adipocytes chondrocytes. MSC were amniotic membranes bone marrows...