A5055330212- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Diet and metabolism studies
- Hearing, Cochlea, Tinnitus, Genetics
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- Hearing Impairment and Communication
- RNA regulation and disease
- NF-κB Signaling Pathways
- Genomics and Chromatin Dynamics
- Medicinal Plant Pharmacodynamics Research
- DNA Repair Mechanisms
- BRCA gene mutations in cancer
- Genetic Syndromes and Imprinting
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Mitochondrial Function and Pathology
- FOXO transcription factor regulation
- Ubiquitin and proteasome pathways
National Cancer Institute
2020-2024
National Institutes of Health
2020-2024
Center for Cancer Research
2019-2024
Government of the United States of America
2022
Universidade de São Paulo
2013-2020
Universidade de Ribeirão Preto
2018-2020
National Council for Scientific and Technological Development
2016-2020
Cancer Institute (WIA)
2020
Instituto Nacional de Tecnologia
2020
Universidade Estadual do Centro-Oeste
2020
Hotair is a member of the recently described class noncoding RNAs called lincRNA (large intergenic RNA). Various studies suggest that acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences ultimately leads suppression several genes. Although has been associated with metastasis and poor prognosis in different tumor types, deep characterization its functions cancer still needed. Here, we investigated role scenario epithelial-to-mesenchymal...
Long noncoding RNAs (lncRNAs) are often associated with polysomes, indicating coding potential. However, only a handful of endogenous proteins encoded by putative lncRNAs have been identified and assigned function. Here, we report the discovery gastrointestinal-tract-specific lncRNA ( LINC00675 ) that is regulated pioneer transcription factor FOXA1 encodes conserved small protein 79 amino acids which termed FORCP FO XA1- R egulated C onserved Small P rotein). transcript undetectable in most...
Abstract Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential function miR-450a, one most significantly downregulated miRNAs ovarian cancer. RNA-seq analysis cancer cell line A2780 revealed that overexpression miR-450a suppressed genes involved epithelial-to-mesenchymal transition (EMT). Overexpression reduced migration and invasion increased...
p53 is an intensely studied tumor-suppressive transcription factor. Recent studies suggest that the RNA-binding protein (RBP) ZMAT3 important in mediating effects of p53. Here, we globally identify ZMAT3-regulated RNAs and their binding sites at nucleotide resolution intact colorectal cancer (CRC) cells. binds to thousands mRNA precursors, mainly intronic uridine-rich sequences affects splicing. The strongest alternatively spliced target was CD44, a cell adhesion gene stem marker controls...
Mitochondria are central key players in cell metabolism, and mitochondrial DNA (mtDNA) instability has been linked to metabolic changes that contribute tumorigenesis increased expression of pro-tumorigenic genes. Here, we use melanoma lines metastatic tumors evaluate the effect mtDNA alterations packaging factor, TFAM, on energetic metabolism nuclear gene changes. We report a positive correlation between copy number, glucose consumption, ATP production lines. Gene analysis reveals...
Laryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be subtype of the head and neck (HNSCC). Despite significant advances in understanding treatment prognosis patients with LSCC has not improved recently. In present study, we sought understand better genetic mechanisms underlying development. Thirty-two tumor samples were collected from undergoing surgical resection LSCC. The submitted whole-genome cDNA microarray analysis aiming identify targets We also...
LINC00629 and MIR503HG are long intergenic non-coding RNAs (lincRNAs) mapped on chromosome X (Xq26), a region enriched for genes associated with human reproduction. Genes highly expressed in normal reproductive tissues cancers (CT genes) well known as potential tumor biomarkers. This study aimed to characterize the structure, expression, function regulation mechanism of lincRNAs. According our data, expression was almost exclusive placenta other tissues. Further analysis, using cancer cell...
Circular RNAs (circRNAs) are a class of noncoding produced by noncanonical form alternative splicing called back-splicing. To investigate potential role circRNAs in the p53 pathway, we analyzed RNA sequencing (RNA-seq) data from colorectal cancer cell lines (HCT116, RKO, and SW48) that were untreated or treated with DNA-damaging agent. Surprisingly, unlike strong p53-dependent induction hundreds p53-induced mRNAs upon DNA damage, only few upregulated genes. circ-MDM2, an annotated circRNA...
Mitochondrial dysfunction is regarded as a hallmark of cancer progression. In the current study, we evaluated mitochondrial genome instability and copy number in colorectal using Next Generation Sequencing approach qPCR, respectively. The results revealed higher levels heteroplasmy depletion relative mtDNA adenocarcinoma. Adenocarcinoma samples also presented an increased mutations nuclear genes encoding proteins which functions are related with mitochondria fusion, fission localization....
Matrin3 is an RNA-binding protein that regulates diverse RNA-related processes, including mRNA splicing. Although has been intensively studied in neurodegenerative diseases, its function cancer remains unclear. Here, we report Matrin3-mediated regulation of mitotic spindle dynamics colorectal (CRC) cells. We comprehensively identified RNAs bound and regulated by CRC cells focused on CDC14B, one the top targets. knockdown results increased inclusion exon containing a premature termination...
Transcription factors play key roles in development and disease by controlling gene expression. Forkhead box A1 (FOXA1), is a pioneer transcription factor essential for mouse functions as an oncogene prostate breast cancer. In colorectal cancer (CRC), FOXA1 significantly downregulated high expression associated with better prognosis, suggesting potential tumor suppressive functions. We therefore investigated the regulation of CRC, focusing on well-differentiated CRC cells, where robustly...
Differentiation status of tumors is correlated with metastatic potential and malignancy. FOXA1 (forkhead box A1) a transcription factor known to regulate differentiation in certain tissues. Here, we investigate
PURPL is a p53-induced lncRNA that suppresses basal p53 levels. Here, we investigated upon activation in liver cancer cells, where it expressed at significantly higher levels than other cell types. Using isoform sequencing, discovered novel transcripts have retained intron and/or previously unannotated exons. To determine function activation, performed transcriptome sequencing (RNA-Seq) after depleting using CRISPR interference (CRISPRi), followed by Nutlin treatment to induce p53....
Despite being a healthy tissue, the constituent cells of placenta, share similar characteristics with tumor cells, such as increased cell growth, migration, and invasion. However, while these processes are stochastic uncontrolled in cancer placenta they precisely controlled. Since miRNAs have been reported to regulate genes that control molecular mechanisms necessary for development both human cancer, we addressed highly expressed could be involved tumorigenesis. Here, assessed miRNA profile...
Susceptibility to breast cancer is significantly increased in individuals with germ line mutations RECQ1 (also known as RECQL or RECQL1), a gene encoding DNA helicase essential for genome maintenance. We previously reported that expression predicts clinical outcomes sporadic patients stratified by estrogen receptor (ER) status. Here, we utilized an unbiased integrative genomics approach delineate cross talk between and ERα, master regulatory transcription factor cancer. found of ESR1, the...
<p>Contains detailed Supplementary Figure and Table legends</p>
<div>Abstract<p>Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential function miR-450a, one most significantly downregulated miRNAs ovarian cancer. RNA-seq analysis cancer cell line A2780 revealed that overexpression miR-450a suppressed genes involved epithelial-to-mesenchymal transition (EMT). Overexpression reduced migration and...
<p>Supplementary Figure 4. miR-450a effects on glutamine uptake and glutamate production.</p>
<p>Supplementary Figure 2. miR-450b similar seed sequence and effects after its overexpression.</p>