A5057719939- Sarcoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Glioma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Ocular Oncology and Treatments
- Cancer-related molecular mechanisms research
- Retinal Development and Disorders
- Cell death mechanisms and regulation
- Force Microscopy Techniques and Applications
- Cancer-related gene regulation
- Planarian Biology and Electrostimulation
- Teratomas and Epidermoid Cysts
- Cancer Mechanisms and Therapy
- Hippo pathway signaling and YAP/TAZ
- Cardiac tumors and thrombi
- Cancer Research and Treatments
- Hedgehog Signaling Pathway Studies
- Tendon Structure and Treatment
- Microtubule and mitosis dynamics
Université de Montréal
2019-2025
Universidade de São Paulo
2013-2024
Institute for Research in Immunology and Cancer
2019-2023
Clinics Hospital of Ribeirão Preto
2020
Universidade de Ribeirão Preto
2017
The protein inhibitor of activated STAT1 (PIAS1) is an E3 SUMO ligase that plays important roles in various cellular pathways. Increasing evidence shows PIAS1 overexpressed human malignancies, including prostate and lung cancers. Here we used quantitative proteomics to identify potential substrates a system-wide manner. We identified 983 sites on 544 proteins, which 62 proteins were assigned as putative substrates. In particular, vimentin (VIM), type III intermediate filament involved...
Epithelial tissues form protective barriers while supporting critical functions such as absorption and secretion. Their structural functional integrity relies on adherens junctions, which coordinate migration transmit forces between adjacent cells by connecting their actin cytoskeleton. In this study, we report the presence of an apical supracellular network in squamous epithelial cells. Using carcinoma A431 a model, characterized composed star-shaped structures interconnected linear bundles...
Abstract Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through egg chamber. To achieve directed motility, BC coordinates formation of protrusions its leader contractility at rear. Restricting to requires actin plasma membrane linker Moesin. Herein, we show Ste20-like kinase Misshapen phosphorylates Moesin vitro BC. Depletion disrupts protrusion restriction, thereby...
Collective cell migration is not only important for development and tissue homeostasis but can also promote cancer metastasis. To migrate collectively, cells need to coordinate cellular extensions retractions, adhesion sites dynamics, forces generation transmission. Nevertheless, the regulatory mechanisms coordinating these processes remain elusive. Using A431 carcinoma cells, we identify kinase MAP4K4 as a central regulator of collective migration. We show that inactivation blocks clusters,...
Despite advances in neurosurgery and aggressive treatment with temozolomide (TMZ) radiation, the overall survival of patients glioblastoma (GBM) remains poor. Vast evidence has indicated that nuclear factor NF- κ B is constitutively activated cancer cells, playing key roles growth survival. Recently, Dehydroxymethylepoxyquinomicin (DHMEQ) shown to be a selective inhibitor antiproliferative properties GBM. In present study, ability DHMEQ surmount tumor's invasive nature therapy resistance...
Abstract We evaluated the potential effects of ATO in different pediatric SHH-MB cell lines (ONS-76: TP53 -wild type; DAOY and UW402: -mutated). MB molecular subgroup was confirmed mutations were validated. Cell viability, clonogenicity apoptosis after treatment at concentrations (1–16 µM) alone or combined with irradiation doses (0.5, 1, 2 4 Gy). Rad51 Ku86 proteins by WB. reduced viability for all lines. Significant decrease clonogenic capacity higher rates also observed exposure, being...
Medulloblastoma (MB) treatment is continuously evolving. Better approaches, focused on particular molecular pathways involved in MB development and progression support new strategies. This article explores the antiproliferative, proapoptotic radiosensitizing effects of Methoxyamine (MX), a base excision repair (BER) inhibitor that has shown anticancer potential by sensitizing tumor cells to ionizing radiation chemotherapy. The DAOY (a desmoplastic cerebellar-derived MB) ONS-76 (classical...
ABSTRACT The Protein Inhibitor of Activated STAT 1 (PIAS1) is an E3 SUMO ligase that plays important roles in various cellular pathways, including signaling, p53 pathway, and the steroid hormone signaling pathway. PIAS1 can SUMOylate PML (at Lys-65 Lys-160) PML-RARα promoting their ubiquitin-mediated degradation. Increasing evidence shows overexpressed human malignancies, prostate lung cancers. To understand mechanism action PIAS1, we developed a quantitative proteomic approach to identify...
ABSTRACT Collective cell migration is important for normal development and tissue homeostasis, but can also promote cancer metastasis. To migrate collectively, cells need to coordinate their protrusion formation, rear retraction, adhesion sites dynamics, as well forces generation transmission. Nevertheless, the regulatory mechanisms coordinating these processes remain elusive. Using A431 carcinoma line, we identify kinase MAP4K4 a central regulator of collective migration. We show that...