A5024684158- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Glycosylation and Glycoproteins Research
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Venomous Animal Envenomation and Studies
- Peptidase Inhibition and Analysis
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Renin-Angiotensin System Studies
- Clinical Nutrition and Gastroenterology
- Metabolism and Genetic Disorders
- Mass Spectrometry Techniques and Applications
- Immunotherapy and Immune Responses
- Aquaculture Nutrition and Growth
- Muscle metabolism and nutrition
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Pancreatitis Pathology and Treatment
- S100 Proteins and Annexins
- Diet and metabolism studies
Universidade de São Paulo
2009-2021
Fundação de Amparo à Pesquisa do Estado de São Paulo
2006-2021
Fundação Hemopa
2006-2017
Universidade de Ribeirão Preto
2002-2017
Clinics Hospital of Ribeirão Preto
1971-2016
Instituto Biológico
2012
Celula (United States)
2012
Universidade Brasil
2011
Universidade Federal de São Paulo
2009-2010
University of Oslo
2010
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton transfer mass spectrometry of peptides. Rapid heating technique for underivatized peptides containing arginineR. J. Beuhler, E. Flanigan, L. Greene, and FriedmanCite this: Am. Chem. Soc. 1974, 96, 12, 3990–3999Publication Date (Print):June 1, 1974Publication History Published online1 May 2002Published inissue 1 June 1974https://pubs.acs.org/doi/10.1021/ja00819a043https://doi.org/10.1021/ja00819a043research-articleACS PublicationsRequest...
The concentrations of angiotensin converting enzyme (ACE) activity, norepinephrine, and serotonin were measured in microdissected regions the dog's brainstem spinal cord. In addition, we determined vitro metabolism 125I-angiotensin I (Ang I) homogenates same brain punch regions. High ACE-specific activity was found monoamine-containing intermediolateral column 125I-Ang metabolized to II (Ang-[1-8]) N-terminal heptapeptide Ang-(1-7). presence MK 422 (50 microM), Ang-(1-7) still generated,...
The most sensitive nonradiometric routine assay for angiotensin-converting enzyme (ACE) activity uses fluorometry to detect His-Leu released from Hip-His-Leu. Our results indicate that, in contrast human serum, rat serum and plasma contain large variable amounts of dipeptidase that lead a subestimation the ACE measured 0.1 M potassium phosphate buffer, pH 8.3, containing 0.3 NaCl, commonly used tissue ACE. We describe validate an 1 10 microL or using 5 mM Hip-His-Leu 500 0.4 sodium borate...
The protein mixture discharged by guinea pig pancreatic lobules incubated in vitro has been analyzed two comple-
ADVERTISEMENT RETURN TO ISSUEPREVArticlePreparation, assay, and partial characterization of a neutral endopeptidase from rabbit brainAntonio C. M. Camargo, Roslyn Shapanka, Lewis J. GreeneCite this: Biochemistry 1973, 12, 9, 1838–1844Publication Date (Print):April 24, 1973Publication History Published online1 May 2002Published inissue 24 April 1973https://pubs.acs.org/doi/10.1021/bi00733a028https://doi.org/10.1021/bi00733a028research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract A trypsin inhibitor has been isolated in 54% yield from bovine pancreatic juice by gel filtration on Sephadex G-75 at pH 8.1 and elution chromatography DEAE-cellulose 9.0. It appears to be homogeneous equilibrium chromatography, sedimentation ultracentrifugation, amino acid analysis, the basis of stoichiometry its interaction with trypsin. The polypeptide a molecular weight 6155 following composition: Asp7, Thr4, Ser2, Glu7, Pro4, Gly5, Ala1, Cys6, Val4, Met1, Ileu3, Leu4, Tyr2,...
Abstract The tryptic peptides of bovine pancreatic secretory trypsin inhibitor (Greene, L. J., and Giordano, J. S., Jr., Biol. Chem., 244, 285 (1969)) have been ordered in a unique sequence by series specific chemical limited enzymatic cleavages the performic acid-oxidized derivative. amino acid is [see PDF for sequence].
Abstract Human pancreatic secretory trypsin inhibitor has been isolated in 55% yield from human juice and 45% post mortem pancreata by gel filtration on Sephadex G-75 the presence of 10-4 m diisopropyl phosphofluoridate ion exchange chromatography DEAE-cellulose SP-Sephadex. The polypeptide a molecular weight 6242 contains 56 amino acid residues per molecule: Asp3, Asn5, Thr4, Ser3, Glu4, Gln2, Pro3, Gly5, Ala1, Cys6, Val2, Ile3, Leu4, Tyr3, Phe1, Lys4, Arg3 does not contain glucosamine or...
SET/I2PP2A is a multifunctional protein that up-regulated in head and neck squamous cell carcinoma (HNSCC). The action of SET HNSCC tumorigenicity unknown. Stable knockdown by shRNA (shSET) was established three lines: HN12, HN13, Cal27. Protein expression phosphorylated levels were determined Western blotting immunofluorescence, migration invasion measured functional analysis, PP2A activity using serine/threonine phosphatase assay. A real-time PCR array used to quantify 84 genes associated...
In order to understand angiotensin metabolism in the canine brain, we determined molecular forms of peptides present hypothalamus dog and carried out measurements 125I-angiotensin I homogenates that tissue. Angiotensin were extracted from hypothalamic tissue quantified by specific radioimmunoassays combined with high-performance liquid chromatography. The major detected angiotensin-(2-7) (391.2 +/- 16.8 pg/g tissue) angiotensin-(3-7) (864.8 128.1 pg/g). II immunoreactivity was mainly...
Abstract To elucidate the osmoregulatory mechanisms underpinning invasion of fresh water by palaemonid Crustacea, we investigate contribution free amino acids (FAA) to intracellular isosmotic regulation in selected ontogenetic stages two diadromous, neotropical shrimps, Macrobrachium amazonicum and M. olfersi, exposed or saline media. We also evaluate anisosmotic/ionic extracellular regulatory capability adult alone; all data for olfersi are from McNamara et al. (2004). Adult shrimps show...
Rabbit brain endo-oligopeptidase B inactivates angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) and II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) by hydrolysis of the Pro7-Phe8 peptide bond. The site was determined in preparative analytical experiments which both products were recovered a molar ratio 1:1, sum plus unhydrolyzed substrate accounted for starting material. enzyme has Km 6.3 x 10(-5) M at pH 8.3 is activated 30-fold with 4.8 mM dithiothreitol. BPP9a ( less than...
Host cell invasion by Toxoplasma gondii is a multistep process with one of the first steps being apical release micronemal proteins that interact host receptors. We demonstrate here protein 1 (MIC1) lactose-binding lectin. MIC1 and MIC4 were recovered in lactose-eluted (Lac+) fraction on affinity chromatography immobilized lactose soluble antigen from tachyzoites virulent RH strain. both identified N-terminal microsequencing. was also sequencing cDNA clones isolated an expression library...
The reduction of circulating neutrophil migration to infection sites is associated with a poor outcome severe sepsis. alpha-1-Acid glycoprotein (AGP) was isolated from the sera severely septic patients by HPLC and acrylamide gel electrophoresis identified mass spectrometry. Both protein commercial AGP inhibited carrageenin-induced into rat peritoneal cavity when administered i.v. at dose 4.0 microg per (95 pmol rat). Analysis intravital microscopy demonstrated that both proteins rolling...