A5009229018- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cancer-related gene regulation
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
- COVID-19 Impact on Reproduction
- Insect and Pesticide Research
- Helminth infection and control
- Healthcare Systems and Public Health
- Biochemical and Molecular Research
- Enzyme Production and Characterization
- Viral Infections and Vectors
- Autoimmune and Inflammatory Disorders Research
Universidade de São Paulo
2019-2024
Universidade de Ribeirão Preto
2021
Universidade Federal de Pelotas
2012-2016
Abstract Leishmania RNA virus (LRV) is an important virulence factor associated with the development of mucocutaneous Leishmaniasis, a severe form disease. LRV-mediated disease exacerbation relies on TLR3 activation, but downstream mechanisms remain largely unexplored. Here, we combine human and mouse data to demonstrate that LRV triggers TRIF induce type I IFN production, which induces autophagy. This process results in ATG5-mediated degradation NLRP3 ASC, thereby limiting inflammasome...
In trypanosomatids, regulation of gene expression occurs mainly at the posttranscriptional level, and RNA-binding proteins (RBPs) are key players in determining fates transcripts. RBPs targets protein arginine methyltransferases (PRMTs), which posttranslationally regulate capacity other RBP interactions by transferring methyl groups to residues (R-methylation). Herein, we functionally characterized five predicted PRMTs Leishmania braziliensis knockout endogenous HA tagging using CRISPR/Cas9...
Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one round of transfection. In addition, development LeishGEdit, a PCR-based toolkit for generating knockouts and tagged lines using CRISPR/Cas9, allowed more straightforward effective editing. this system, plasmid pTB007 is delivered episomal...
Effective control of gastrointestinal parasites is necessary in sheep production. The development anthelmintics resistance causing the available chemically based to become less effective. Biological strategies present an alternative this problem. In current study, we tested larvicidal effects Bacillus thuringiensis var. israelensis Cry11Aa toxin against Haemonchus contortus larvae. Bacterial suspensions [2 × 108 colony-forming units (CFU) g-1 feces] B. and recombinant Escherichia coli...
In the protozoan parasite Leishmania , most genes encoding for ribosomal proteins (RPs) are present as two or more copies in genome. However, their untranslated regions (UTRs) predominantly divergent and might be associated with a distinct regulation of expression paralogous genes. Herein, we investigated profiles RPs (S16 L13a) encoded by duplicated major . The S16 protein possess identical coding sequences (CDSs) UTRs, whereas CDSs L13a diverge amino acids UTRs. Using CRISPR/Cas9 genome...
Leishmania major is the main causative agent of cutaneous leishmaniasis in Old World. In parasites, lack transcriptional control mostly compensated by post-transcriptional mechanisms. Methylation arginine a conserved post-translational modification executed Protein Arginine Methyltransferase (PRMTs). The genome from L . encodes five PRMT homologs, including cytosolic protein associated with several RNA-binding proteins, LmjPRMT7 It has been previously reported that could impact parasite...
Abstract In the protozoan parasite Leishmania, most of genes encoding for ribosomal proteins (RPs) are present as two or more copies in genome, their untranslated regions (UTRs) predominantly divergent, and might be associated with a distinct regulation paralogous genes’ expression. Here, we investigated expression profiles RPs (S16 L13a) encoded by duplicated Leishmania major. The S16 protein have identical CDSs divergent UTRs while L13a diverge amino acids UTRs. Using CRISPR/Cas9 genomic...
Abstract In trypanosomatids, regulation of gene expression occurs mainly at the posttranscriptional level, and RNA-binding proteins (RBPs) are key players in determining fates transcripts. RBPs major targets protein arginine methyltransferases (PRMTs), which posttranslationally regulate capacity other macromolecular interactions by transferring methyl groups to residues. Herein, we present results a study that functionally characterized five predicted PRMTs Leishmania braziliensis knockout...