A5064342858- Listeria monocytogenes in Food Safety
- Biosensors and Analytical Detection
- Microbial Inactivation Methods
- Mycobacterium research and diagnosis
- Identification and Quantification in Food
- Milk Quality and Mastitis in Dairy Cows
- Essential Oils and Antimicrobial Activity
- HIV Research and Treatment
- Botulinum Toxin and Related Neurological Disorders
- Leptospirosis research and findings
- Tuberculosis Research and Epidemiology
- Microbial Metabolism and Applications
- Veterinary medicine and infectious diseases
- HIV/AIDS drug development and treatment
- Hereditary Neurological Disorders
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Advanced Biosensing Techniques and Applications
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Infections and bacterial resistance
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
Universidade Federal de Pernambuco
2020-2025
Universidade Federal do Agreste de Pernambuco
2020-2025
Universidade Federal de Pelotas
2007-2024
Universidade Federal Rural de Pernambuco
2016-2022
University of Warwick
2020
Rural Resources
2019
Hospital Santa Paula
2015-2018
Purdue University West Lafayette
2011-2016
Instituto de Infectologia Emílio Ribas
2007-2015
Hospital Universitário da Universidade de São Paulo
2012
Abstract Background Immunomagnetic separation (IMS) and immunoassays are widely used for pathogen detection. However, novel technology platforms with highly selective antibodies essential to improve detection sensitivity, specificity performance. In this study, monoclonal (MAbs) against Internalin A (InlA) p30 were generated on paramagnetic beads of varying diameters concentration, as well fiber-optic sensor Results Anti-InlA MAb-2D12 (IgG2a subclass) was specific Listeria monocytogenes L....
Listeria monocytogenes is a foodborne pathogen that has become an important cause of human and animal diseases worldwide. The purpose this study was to evaluate the serotypes, virulence potential, antimicrobial resistance profile, genetic relationships 50 L. isolates from food environment in southern Brazil. In study, majority belonged serotypes 1/2b (42%) 4b (26%), which are main associated with listeriosis. addition, all harboured internalin genes (inlA, inlC, inlJ), indicating potential....
Abstract Clostridium perfringens is an anaerobic bacterium that produces several toxins. Of these, the alpha, beta, and epsilon toxins are responsible for causing most severe C. -related diseases in farm animals. The best way to control these through vaccination. However, commercially available vaccines based on inactivated have many production drawbacks, which can be overcome use of recombinant antigens. In this study, we produced Escherichia coli formulate a trivalent vaccine. Its...
Bovine botulism is a fatal disease that caused by botulinum neurotoxins (BoNTs) produced Clostridium serotypes C and D causes great economic losses, with nearly 100% lethality during outbreaks. It has also been considered potential source of human food-borne illness in many countries. Vaccination reported to be the most effective way control bovine botulism. However, commercially available toxoid-based vaccines are difficult hazardous produce. Neutralizing antibodies targeted against...
The identification of potential vaccine candidates against leptospirosis remains a challenge. However, one such candidate is OmpL37, potentially surface-exposed antigen that has the highest elastin-binding ability described to date, suggesting it plays an important role in host colonization. In order evaluate OmpL37's induce protective immune response, prime-boost, DNA and subunit strategies were tested hamster model lethal leptospirosis. humoral response was evaluated using indirect ELISA...
Resumo A indigestão vagal (IV) é uma síndrome caracterizada por alterações na motilidade dos pré-estômagos e do abomaso, resultante de disfunções no nervo vago. Embora diversas condições inflamatórias estejam associadas à lesão nervosa, a relação entre abscessos peri-hepáticos IV ainda pouco documentada. Diante disto, realizou-se um estudo retrospectivo achados clínicos, laboratoriais, ultrassonográficos anatomopatológicos 15 bovinos diagnosticados com em decorrência abscesso peri-hepático....
Abstract Vagal indigestion (VI) is a syndrome characterized by changes in the motility of forestomach and abomasum, which occurs event dysfunctions vagus nerve. Several inflammatory conditions are associated with nerve injury, however, relationship between perihepatic abscesses this poorly documented. Therefore, retrospective study clinical, laboratory, ultrasound anatomopathological findings 15 cattle presented VI due to abscess was carried out. Samples from liver three were sent for...
To determine whether cognitive performance from infancy to adulthood is affected by being born small for gestational age (SGA), and if this depends on the SGA reference used. Furthermore, SGA's effect while considering effects of very preterm/very low birthweight (VP/VLBW), socio-economic status (SES) parent-infant relationship.A total 414 participants (197 term-born, 217 VP/VLBW) Bavarian Longitudinal Study.Small was classified using neonatal or fetal growth references. SES relationship...
The detection of Listeria monocytogenes, a Gram-positive food-borne bacterium, helps prevent listeriosis outbreaks, an invasive disease with high mortality rates among immunocompromised individuals. Microbiological culture methods are time-consuming, requiring laboratory infrastructure while molecular techniques expensive and require complex equipments. We have developed point care test (POCT) based on lateral flow immunochromatography, combining anti-Internalin A B antibodies the...
Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding surface exposed antigens on would provide potential diagnostic therapeutic targets. In present work, using mass spectrometry genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II species antigen target previously described mAb-3F8. Western dot blot assays confirmed mAb-3F8 could distinguish...
Abstract Some individuals are resistant to HIV-1 infection despite repeated exposure the virus, suggesting presence of a complex antiviral response. Innate factors like IL-22 exert gut mucosal protection and polyfunctional T cells have been associated with low progression in HIV infection; therefore, we evaluated frequencies CD4+ CD8+ cell-secreting cytokines, including Tc22/Th22 HIV-1-exposed uninfected (EUs), their HIV-1-infected partners healthy controls. EUs exhibited an increased...
The genus Listeria comprises ubiquitous bacteria, commonly present in foods and food production facilities. In this study, three different phage display technologies were employed to discover targets, generate characterize novel antibodies against Listeria: antibody for biomarker discovery generation; ORFeome target identification; single-gene epitope characterization. With approach, pyruvate dehydrogenase complex-enzyme 2 (PDC-E2) was defined as a new detection Listeria, confirmed by...
Brazilian AIDS and HIV-1-seropositive patients have had free access to highly active antiretroviral therapy (HAART) since November 1996. Although secondary data based on official mortality statistics indicate a sharp decrease in mortality, few if any studies tried estimate the prognosis for with HIV who been followed from beginning of HAART era. An observational study, retrospective prospective components, was done 233 adult HIV-1-infected subjects were recruited last 10 years at outpatient...