A5084957238- Biosensors and Analytical Detection
- Transgenic Plants and Applications
- Listeria monocytogenes in Food Safety
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Identification and Quantification in Food
- Toxoplasma gondii Research Studies
- Plant tissue culture and regeneration
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Plant and Fungal Interactions Research
- Salmonella and Campylobacter epidemiology
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Insect symbiosis and bacterial influences
- Respiratory viral infections research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Herpesvirus Infections and Treatments
- Infectious Diseases and Tuberculosis
- Microbial Inactivation Methods
- Mycobacterium research and diagnosis
- Viral Infectious Diseases and Gene Expression in Insects
Universidade Federal de Goiás
2020-2025
Universidade de Brasília
2018-2019
The detection of Listeria monocytogenes, a Gram-positive food-borne bacterium, helps prevent listeriosis outbreaks, an invasive disease with high mortality rates among immunocompromised individuals. Microbiological culture methods are time-consuming, requiring laboratory infrastructure while molecular techniques expensive and require complex equipments. We have developed point care test (POCT) based on lateral flow immunochromatography, combining anti-Internalin A B antibodies the...
Lateral flow immunoassays (LFIA) for antibody detection represent cost-effective and user-friendly tools serology assessment. This study evaluated a new LFIA prototype developed with recombinant chimeric antigen from the spike/S nucleocapsid/N proteins to detect anti-SARS-CoV-2 IgG antibodies. The evaluation of sensitivity specificity used 811 serum samples 349 hospitalized, SARS-CoV-2 RT-qPCR positive COVID-19 patients, collected at different time points 193 healthy controls. agreement...
This study presents the development of a polyester microplate for detecting S-protein SARS-CoV-2 virus in saliva and nasopharyngeal swab samples using direct enzyme-linked immunosorbent assay (ELISA) technology. The was designed to contain 96 zones with 3 mm diameter each, volume 2-3 μL. experimental conditions including reagent concentration reaction time were optimized. image digitized analyzed graphical software. linear range obtained between protein S concentrations pixel intensity 0-10 μg mL
Lateral flow immunoassays (LFIA) for antibody detection represent cost-effective and user-friendly tools serology assessment. This study evaluated a new LFIA prototype that detects anti-SARS-CoV-2 IgG antibodies using recombinant chimeric antigen from the spike/S nucleocapsid/N proteins. 1044 serum samples 349 hospitalized SARS-CoV-2 PCR positive COVID-19 patients, collected at different time points 193 healthy control individuals were used evaluation of sensitivity specificity. Also 40...
A plant-based heterologous expression system is an attractive option for recombinant protein production because of its based on a eukaryotic high feasibility, and low biological risks. Frequently, binary vector systems are used transient gene-expression in plants. However, plant virus vector-based offer advantages higher yields due to their self-replicating machinery. In the present study, we show efficient protocol using tobravirus, pepper ringspot virus, that was employed severe acute...